《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (5): 1491-1498.doi: 10.16431/j.cnki.1671-7236.2019.05.028

• 基础兽医 • 上一篇    下一篇

乳黄消口服液滋阴、扶正及祛邪功效的研究

白东东, 李新圃, 杨峰, 罗金印, 王旭荣, 李宏胜   

  1. 中国农业科学院兰州畜牧与兽药研究所, 农业部兽用药物创制重点实验室, 甘肃省新兽药工程重点实验室, 兰州 730050
  • 收稿日期:2018-11-06 出版日期:2019-05-20 发布日期:2019-05-20
  • 通讯作者: 李宏胜 E-mail:lihsheng@sina.com
  • 作者简介:白东东(1992-),男,陕西延安人,硕士生,研究方向:中兽医临床,E-mail:baidd163@hotmail.com
  • 基金资助:

    国家重点研发项目"畜禽群发普通病防控技术研究"(2017YFD0502200);甘肃省国际科技合作项目(17YF1WA169);中国农业科学院奶牛疾病研究创新工程项目(CAAS-ASTIP-2014-LIHPS-03)

Study on the Effect of Yin-nourishing,Strengthening Immune and Dispelling Evil by Ru Huangxiao Oral Liquid

BAI Dongdong, LI Xinpu, YANG Feng, LUO Jinyin, WANG Xurong, LI Hongsheng   

  1. 1. Key Laboratory of Veterinary Pharmaceutical Discovery, Ministry of Agriculture, Key Laboratory of New Animal Drug Project of Gansu Province, Lanzhou Institute of Animal Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China
  • Received:2018-11-06 Online:2019-05-20 Published:2019-05-20

摘要:

为探讨乳黄消口服液滋阴、扶正及祛邪的功效,本研究观察乳黄消口服液对阴虚模型大鼠临床症状、饮食量、饮水量、体重、胸腺指数、脾脏指数、总三碘甲状腺原氨酸(T3)、总甲状腺素(T4)和促甲状腺激素(TSH)含量的影响,以及对小鼠巨噬细胞吞噬功能及对大肠杆菌、金黄色葡萄球菌、无乳链球菌和副乳房链球菌抑制作用的影响。结果显示,试验组大鼠在乳黄消口服液给药后精神正常,易抓取,但被毛光泽仍较差,有脱毛现象,大便正常。与模型组相比,试验组大鼠体重(P>0.05)、胸腺指数(P>0.05)、脾脏指数(P>0.05)、TSH含量(P<0.05)上升,饮食量(P<0.05)、T3P<0.05)、T4含量(P>0.05)下降,但均与阴性对照组差异不显著(P>0.05);与模型组相比,试验组大鼠饮水量显著下降(P<0.05),但仍显著高于阴性对照组(P<0.05)。乳黄消口服液给药后高、中、低剂量组与阴性对照组间吞噬指数均差异显著(P<0.05),乳黄消口服液高、中剂量组与阴性对照组相比吞噬百分比差异显著(P<0.05);乳黄消口服液对大肠杆菌、金黄色葡萄球菌、无乳链球菌及副乳房链球菌的最小抑菌浓度(MIC)分别为12.50、1.56、0.20和0.39 mg/mL。综上所述,乳黄消口服液可改善大鼠阴虚临床症状,增强巨噬细胞吞噬功能,抑制奶牛乳房炎主要病原菌,具滋阴、扶正和祛邪的功效,可用于奶牛乳房炎的治疗。

关键词: 乳黄消口服液; 阴虚模型; 巨噬细胞; 抗菌活性

Abstract:

To explore the effects of Ru Huangxiao oral liquid on yin-nourishing, strengthening righteousness and eliminating evil spirits, this study observed the clinical symptoms,diet,water consumption, body weight, thymus index, spleen index, contents of total triiodothyronine (T3), total thyroxine (T4) and thyroid stimulating hormone (TSH) in rats with yin deficiency model and the effect of mouse macrophage phagocytosis and inhibition on Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae and Streptococcus uberis. The results showed that the rats of yin deficiency model had normal mental state and were easy to grasp after the administration of Ru Huangxiao oral liquid, but the gloss of the coat was still poor, there was hair removal and the stool was normal. Compared with model group, the body weight (P>0.05), thymus index (P>0.05), spleen index (P>0.05), TSH content (P<0.05) of the experimental group increased, the food intake (P<0.05), T3 (P<0.05) and T4 content (P>0.05) decreased. However, the difference was not significantly different from the negative control group (P>0.05).Compared with model group, the water consumption of the rats in the experimental group significantly decreased (P<0.05), but it was still significantly higher than the negative control group (P<0.05). The phagocytic index of the high, middle and low dose groups and the negative control group were significantly different (P<0.05). The percentage of phagocytosis was significantly higher in the high-dose and middle-dose groups compared with the negative control group (P<0.05). The minimum inhibitory concentration (MIC) of Ru Huangxiao oral liquid to Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae and Streptococcus parauberis were 12.50, 1.56, 0.20 and 0.39 mg/mL, respectively. In summary, the Ru Huangxiao oral liquid could improve the clinical symptoms of yin deficiency in rats and enhance the phagocytic function of macrophages. At the same time, it could inhibit the main pathogens of cow mastitis, and had the effects of nourishing yin, strengthening righteousness and eliminating evil spirits, it could be used for the treatment of dairy cow mastitis.

Key words: Ru Huangxiao oral liquid, model of yin deficiency, macrophage, antibacterial activity

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