《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (5): 1405-1412.doi: 10.16431/j.cnki.1671-7236.2019.05.018

• 遗传繁育 • 上一篇    下一篇

绵羊Tacr3基因多态性与产羔数的关联分析

陈炜昊1,2, 田志龙1,3, 孙伟2, 储明星1   

  1. 1. 中国农业科学院北京畜牧兽医研究所, 农业农村部动物遗传育种与繁殖重点实验室, 北京 100193;
    2. 扬州大学动物科学与技术学院, 扬州 225009;
    3. 河南科技大学动物科技学院, 洛阳 471003
  • 收稿日期:2018-11-01 出版日期:2019-05-20 发布日期:2019-05-20
  • 通讯作者: 孙伟, 储明星 E-mail:dkxmsunwei@163.com;mxchu@263.net
  • 作者简介:陈炜昊(1995-),男,江苏无锡人,硕士生,研究方向:动物遗传育种,E-mail:512105098@qq.com
  • 基金资助:

    国家自然科学基金项目(31772580、31472078);国家肉羊产业技术体系专项(CARS-38);中国农业科学院科技创新工程(ASTIP-IAS13);中央级公益性科研院所基本科研业务费专项(Y2017JC24);农业科研杰出人才及其创新团队项目(农办人[2015]62号);国家万人计划科技创新领军人才项目(W02020274);天津市农业科技成果转化与推广项目(201704020)

Polymorphism of Tacr3 Gene and Its Association with Litter Size in Sheep

CHEN Weihao1,2, TIAN Zhilong1,3, SUN Wei2, CHU Mingxing1   

  1. 1. Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    3. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China
  • Received:2018-11-01 Online:2019-05-20 Published:2019-05-20

摘要:

本研究旨在探究绵羊速激肽受体3(tachykinin receptor 3,Tacr3)基因g.21484478A > C、g.21560640C > T和g.21560688T > C位点多态性与绵羊产羔数之间的关系,为绵羊高繁殖力分子育种提供新的遗传标记。利用全基因组重测序结合Sequenom MassARRAY® SNP技术对常年发情绵羊品种(小尾寒羊、湖羊和策勒黑羊)和季节性发情绵羊品种(滩羊、苏尼特羊和草原型藏羊)Tacr3基因3个多态位点进行检测,并与小尾寒羊产羔数进行关联分析。结果表明,3个位点均存在3种基因型,其中g.21484478A>C位点存在AA、CC和CA基因型,g.21560640C>T位点存在TT、CC和TC基因型,g.21560688T>C位点存在TT、CC和CT基因型,3个多态位点基因型频率和等位基因频率在两种发情模式绵羊品种间均达到差异极显著水平(P<0.01)。群体遗传学分析表明,g.21484478A>C位点在滩羊和策勒黑羊中均表现为中度多态(0.25 < PIC < 0.5),在小尾寒羊、苏尼特羊和湖羊中均为低度多态(PIC<0.25);g.21560640C>T位点在6个绵羊品种中均表现为中度多态;g.21560688T>C位点在小尾寒羊、苏尼特羊、湖羊和草原型藏羊中均表现为低度多态。卡方适合性检验表明,g.21560640C>T位点在6个绵羊品种中均处于哈代-温伯格平衡状态(P>0.05);g.21484478A>C位点在草原型藏羊中处于哈代-温伯格不平衡状态(P<0.05);g.21560688T>C位点在滩羊、策勒黑羊中处于哈代-温伯格不平衡状态(P<0.05)。关联分析表明,3个多态位点与小尾寒羊第一、二、三胎产羔数均无显著关联(P>0.05)。推测Tacr3基因3个多态位点均不适用于小尾寒羊产羔数选育。

关键词: 绵羊; Tacr3基因; 分型; 产羔数

Abstract:

The aim of this study was to elucidate the association between tachykinin receptor 3 (Tacr3) gene polymorphism (g.21484478A>C,g.21560640C>T and g.21560688T>C loci) and litter size in sheep,providing new genetic markers for high prolific molecular breeding of sheep.Year-round estrous sheep breeds (Small-tail Han sheep,Hu sheep and Cele Black sheep) and seasonal estrous sheep breeds (Tan sheep,Sunite sheep and Prairie Tibetan sheep) were selected and Sequenom MassARRAY® SNP assay was applied to detect the genotypes of g.21484478A > C,g.21560640C > T and g.21560688T > C loci of Tacr3 gene,then the association was analyzed between Tacr3 gene polymorphism and litter size in Small-tail Han sheep.The results showed that three genotypes were found at g.21484478A>C (AA,CA and CC),g.21560640C>T (TT,TC and CC) and at g.21560688T>C (TT,CT and CC) loci in both year-round estrous and seasonal estrous sheep,both genotype frequency and gene frequency of three loci of Tacr3 gene were extremely significantly different between year-round estrous and seasonal estrous sheep (P<0.01).Population genetic analysis showed that the g.21484478A>C locus was at moderate polymorphism (0.25 < PIC < 0.5) in Tan sheep and Cele Black sheep,it was at low polymorphism (PIC<0.25) in Small-tail Han sheep,Sunite sheep and Hu sheep;The g.21560640C>T locus was at moderate polymorphism in 6 sheep breeds;The g.21560688T>C locus was at low polymorphism in Small-tail Han sheep,Sunite sheep,Hu sheep and Prairie Tibetan sheep.The χ2 test indicated that the g.21560640C>T locus of 6 sheep breeds was in Hardy-Weinberg equilibrium (P>0.05);The g.21484478A>C locus of Prairie Tibetan sheep was not in Hardy-Weinberg equilibrium (P<0.05);The g.21560688T>C locus of Tan sheep and Cele Black sheep was not in Hardy-Weinberg equilibrium (P<0.05).The association analysis indicated that the polymorphism of g.21484478A > C,g.21560640C > T and g.21560688T > C had no correlation with the litter size of the first,second or third parity in Small-tail Han sheep (P>0.05).In conclusion,the three polymorphic loci of Tacr3 gene might not be suitable for breeding of Small-tailed Han sheep.

Key words: sheep, Tacr3 gene, genotyping, litter size

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