《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (5): 1362-1369.doi: 10.16431/j.cnki.1671-7236.2019.05.013

• 动物营养与饲料科学 • 上一篇    下一篇

日粮中添加凝结芽孢杆菌对仔猪血浆抗氧化指标和肠黏膜生长的影响

余魁1, 张越1, 李思源1, 纪昌正1, 董毅1, 赵广宇1, 王蕾1, 赵迪1, 易丹1,2, 侯永清1,2   

  1. 1. 武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心, 武汉 430023;
    2. 武汉轻工大学动物营养与饲料科学湖北省重点实验室, 武汉 430023
  • 收稿日期:2018-10-29 出版日期:2019-05-20 发布日期:2019-05-20
  • 通讯作者: 侯永清 E-mail:houyq@aliyun.com
  • 作者简介:余魁(1993-),男,湖北武汉人,硕士生,研究方向:营养生化与代谢调控,E-mail:553765831@qq.com
  • 基金资助:

    国家重点研发计划课题(2016YFD0501210)

Effects of Bacillus coagulans on Plasma Antioxidant Indexes and Intestinal Mucosal Growth of Piglets

YU Kui1, ZHANG Yue1, LI Siyuan1, JI Changzheng1, DONG Yi1, ZHAO Guangyu1, WANG Lei1, ZHAO Di1, YI Dan1,2, HOU Yongqing1,2   

  1. 1. Engineering Research Center of Feed Protein Resources on Agricultural By-products, Ministry of Education, Wuhan Polytechnic University, Wuhan 430023, China;
    2. Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China
  • Received:2018-10-29 Online:2019-05-20 Published:2019-05-20

摘要:

试验研究了日粮中添加凝结芽孢杆菌对仔猪血浆抗氧化指标和肠黏膜生长的影响。选取30头体重相近的21日龄健康断奶仔猪随机分为3组(对照组、试验Ⅰ组和试验Ⅱ组),每组10个重复,每个重复1头猪。对照组饲喂基础日粮,试验Ⅰ、Ⅱ组在基础日粮的基础上分别添加2.0×106、2.0×107 CFU/g的凝结芽孢杆菌。正试期21 d,试验结束时前腔静脉采血后屠宰取样。结果显示:与对照组相比,试验Ⅰ和Ⅱ组断奶仔猪血浆超氧化物歧化酶(SOD)活性、空肠和回肠绒毛高度与隐窝深度的比值、回肠总蛋白(TP)含量及RNA与DNA的比值均显著提高(P<0.05);试验Ⅱ组仔猪血浆丙二醛(MDA)含量显著降低(P<0.05);试验Ⅰ组空肠TP含量及TP/DNA显著提高(P<0.05)。在本试验条件下,日粮中添加凝结芽孢杆菌可以提高断奶仔猪的抗氧化能力,促进仔猪肠道黏膜细胞的增殖与更新,从而改善了肠道黏膜形态结构。

关键词: 凝结芽孢杆菌; 断奶仔猪; 血浆抗氧化; 肠黏膜形态; 肠黏膜生长

Abstract:

This study was conducted to determine the effects of dietary Bacillus coagulans supplementation on plasma antioxidant indexes and intestinal mucosal growth of piglets.Thirty healthy weaned piglets (21-day-old) were randomly divided into three experimental groups (10 replicates per group):Control group,group Ⅰ and group Ⅱ.The control group was fed the basal diet,and the experimental groups Ⅰ and Ⅱ were fed the basal diet+2.0×106 and 2.0×107 CFU/g of Bacillus coagulans,respectively.On day 21 of the trail,blood samples were collected and then all the piglets were sacrificed and intestinal mucosa were collected for analysis.The results showed that:Compared with control group,the plasma superoxide dismutase (SOD) activity,the ratio of villus height to crypt depth in jejunum and ileum,the ileal TP content and the ratios of RNA to DNA of pigs in both groups Ⅰand Ⅱ were significantly increased (P<0.05); The plasma malondialdehyde (MDA) content of pigs in group Ⅱ was significantly decreased (P<0.05), while the TP content and the ratio of TP to DNA in jejunum of pigs in group Ⅰ increased significantly (P<0.05).In conclusion,under the conditions of this experiment,dietary supplementation with Bacillus coagulans could enhance the antioxidant capacity of weaned piglets,promote the proliferation and renewal of intestinal mucosal cells,and improve the intestinal mucosal morphology.

Key words: Bacillus coagulans, weaned piglets, plasma antioxidant indexes, intestinal mucosal morphology, intestinal mucosal growth

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