《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (5): 1316-1322.doi: 10.16431/j.cnki.1671-7236.2019.05.008

• 生理生化 • 上一篇    下一篇

骆驼尿液对预防CCl4诱导小鼠肝损伤的保护作用

浩斯娜1, 卓娜1, 海勒1, 伊丽1, 明亮1, 吉日木图1,2,3   

  1. 1. 内蒙古农业大学乳品生物技术与工程教育部重点实验室, 呼和浩特 010018;
    2. 内蒙古骆驼保护学会, 呼和浩特 010018;
    3. 内蒙古骆驼研究院, 巴丹吉林 737300
  • 收稿日期:2018-09-18 出版日期:2019-05-20 发布日期:2019-05-20
  • 通讯作者: 明亮, 吉日木图 E-mail:bmlimau@163.com;yeluotuo1999@vip.163.com
  • 作者简介:浩斯娜(1993-),女,内蒙古鄂尔多斯人,研究生,研究方向:乳制品生物技术与工程,E-mail:hsnimau@163.com
  • 基金资助:

    国家自然科学基金项目(31360397);内蒙古自然科学基金(2018BS03017);国际科技合作项目(2015DFR30680、ky201401002)

Protective Effect of Camel Urine on Liver Injury Induced by CCl4 in Mice

HAO Sina1, ZHUO Na1, HAI Le1, YI Li1, MING Liang1, JIRIMUTU1,2,3   

  1. 1. Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Inner Mongolia Camel Conservation Society, Hohhot 010018, China;
    3. Inner Mongolia Institute of Camel Research, Badanjiran 737300, China
  • Received:2018-09-18 Online:2019-05-20 Published:2019-05-20

摘要:

试验旨在研究骆驼尿液对预防四氯化碳(CCl4)诱导小鼠肝损伤的保护作用。将40只C57BL/6小鼠随机分为正常组,模型组,骆驼尿液低剂量组(170 mg/kg)、中剂量组(340 mg/kg)和高剂量组(500 mg/kg),3组骆驼尿液组灌胃相应剂量的骆驼尿液,正常组和模型组灌胃等量的生理盐水,每日灌胃1次,连续14 d;试验末期,除正常组外(橄榄油),各组小鼠腹腔注射20% CCl4橄榄油混合溶液,建立小鼠急性肝损伤模型;12 h后摘眼球取血和采集肝脏样本,并记录肝脏重量,计算肝脏指数;测定小鼠血清相关指标,并进行肝脏组织病理切片检查。结果显示,与模型组比较,低、中、高剂量骆驼尿液组小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性及总胆红素(TBIL)、碱性磷酸酶(ALP)、葡萄糖(GLU)含量较模型组均明显降低,白蛋白(ALB)、总蛋白(TP)含量升高,且高剂量组效果较好;而小鼠肝脏病理学切片观察显示,骆驼尿液组均有不同程度的改善,其中高剂量骆驼尿液组小鼠肝脏组织病理学损伤明显减轻,与正常组的肝脏组织形态接近。比较低、中、高3种浓度骆驼尿液处理发现,浓度越高治疗效果越好,说明骆驼尿液治疗效果具有剂量依赖性。综上所述,骆驼尿液对CCl4诱导小鼠肝损伤具有一定的预防保护作用,且高剂量骆驼尿液组的效果较好。

关键词: 骆驼尿液; 四氯化碳(CCl4); 肝损伤; 预防作用

Abstract:

In this study,protective effect of camel urine on liver injury induced by CCl4 in mice was investigated.Forty mice were randomly divided into five groups:Normal group,model group,low-dose camel urine group (170 mg/kg),medium dose camel urine group (340 mg/kg) and high dose camel urine group (500 mg/kg),respectively.The normal group and model group received the same amount of saline infusions once a day,while 3 camel urine groups received different amount of camel urine.All mice were given continuous administration for 14 d and injected with 20% CCl4 and olive oil solution to establish an acute liver injury model except the control group at the end of the experiment.Mice have sacrificed after 12 h post-CCl4 injection when blood and livers were collected,and the liver weight and the liver index were recorded and calculated.Then the related serum indicators and the pathological examination of liver tissue were detected.The results showed that compared with the model group,the level of serum ALT,AST,TBIL,ALP,GLU,TP in low,middle,high dose camel urine groups were decreased,while the serum ALB level was increased with high dose group had a better effect.According to the results of the pathological section,the liver histopathological damage of mice in 3 camel urine groups were all alleviate,and that in the high dose camel urine group was very similar to the normal group.In addition,it could be seen from the results that among the three of camel urine groups,the higher concentration had a better therapeutic effect,indicating that the effect of camel urine was dose-dependent.In summary,camel urine had a certain preventive effect on CCl4-induced liver injury in mice,and the high dose was the better.

Key words: camel urine, carbon tetrachloride (CCl4), liver injury, prevention

中图分类号: