一例鸭疫里默氏杆菌病的诊断及病原分离鉴定

doi:10.16431/j.cnki.1671-7236.2019.04.026

摘要/Abstract

摘要：

河南商丘一肉鸭养殖场疑似发生鸭疫里默氏杆菌病，为确定其病原，无菌采集病死鸭的大脑、肝脏、脾脏、肺脏等组织，依据常规PCR检测方法进行低致病性禽流感H9（AIV-H9）、鸭坦布苏病毒（DTMUV）、鸭呼肠孤病毒（DRV）、新城疫病毒（NDV）等鸭群常见病毒病的核酸检测，利用血清琼脂平板进行细菌分离鉴定，玻片凝集鉴定分离菌株血清型，PCR检测鉴定后进行动物回归试验。并进行31种抗菌药物的敏感试验。结果显示，AIV-H9、TMUV、DRV及NDV核酸检测全部为阴性，初步排除这4种病毒的感染。无菌挑取发病鸭肝脏及脑组织于血清营养琼脂培养基中培养，发现培养基中呈现针尖大小、半透明的菌落。取纯化培养的菌液进行PCR扩增显示，扩增出338 bp的鸭疫里默氏杆菌（RA）特异性条带，血清型鉴定结果为RA血清7型。动物回归试验结果显示，该分离菌株可在临床上被成功复制，能够表现出明显的传染性浆膜炎临床感染症状。药物敏感性试验显示，该血清型RA对头孢克肟、头孢唑林、头孢拉定、头孢曲松、大观霉素、多西环素、左氧氟沙星、氟苯尼考、林可霉素、氨苄西林（舒巴坦）高度敏感。综上所述，造成该肉鸭场疾病感染的病原为血清7型RA，建议使用头孢菌素类等高敏药物进行治疗。

关键词: 鸭疫里默氏杆菌(RA); 分离鉴定; 药敏试验; 血清型; 动物回归

Abstract:

In order to diagnose the pathogen which caused disease of one duck farm in Shangqiu in Henan province,the brain,liver,spleen,and lungs of diseased ducks were collected,and AIV-H9,DTMUV,DRV and NDV were tested according to PCR detection methods of nucleic acid.Isolation and identification of bacteria was operated by serum-agar plates and conventional PCR detection methods.The serotype of the isolated strain was identified by glass agglutination,and the animal regression test was conducted.In order to screen sensitive drugs of the pathogen,31 kinds of antibacterials were chosen in the drug sensitivity test.The results showed that the detections of AIV-H9,TMUV,DRV and NDV were negative,which means these four viral diseases was initially excluded.The liver and brain tissues were cultured in serum nutrient AGAR medium aseptically and there were a translucent colonies at the size of a needle on culture medium.PCR amplification of purified culture broth showed that 338 bp specific bands of RA were amplified which was identified as RA serotype 7.The animal regression test showed that the isolated strain could be successfully replicated in clinical practice and could show obvious clinical infection symptoms of infectious serositis.The drug sensitivity test results showed that the isolates were sensitive to cefixime,cefazolin,cefradine,ceftriaxone,spectinomycin,doxycycline,levofloxacin,florfenicol,lincomycin and ampicillin (sulbactam).In conclusion,the pathogen causing the disease in the duck farm was Riemerella anatipestifer serotype 7 and some antibacterials could be used such as cephalosporin antibiotics.

Key words: Riemerella anatipestifer (RA); isolation and identification; drug sensitivity test; serotypes; animal regression test

中图分类号:

S852.61

郝东敏, 钟洪义, 程冰花, 鲁爱玲, 许传宝, 马有全. 一例鸭疫里默氏杆菌病的诊断及病原分离鉴定[J]. 《中国畜牧兽医》, 2019, 46(4): 1174-1180.

HAO Dongmin, ZHONG Hongyi, CHENG Binghua, LU Ailing, XU Chuanbao, MA Youquan. Diagnosis of Riemerella anatipestifer Infection and Identification of the Pathogens[J]. , 2019, 46(4): 1174-1180.

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