山羊皮下脓肿的病原分离与鉴定

doi:10.16431/j.cnki.1671-7236.2019.03.037

摘要/Abstract

摘要：

为了解引起山羊皮下脓肿的主要病原，本研究从四川乐至县、金堂县和蓬溪县3个山羊养殖场无菌采集患皮下脓肿病羊脓液样本19份，通过细菌分离培养、生化试验及特异性PCR方法对其病原进行检测和鉴定，并通过药敏试验测定分离病原的药物敏感性。结果显示，PCR扩增法共检测出病原菌19株，其中伪结核棒状杆菌、金黄色葡萄球菌及化脓隐秘杆菌分离率分别为57.9%（11/19）、26.3%（5/19）及15.8%（3/19）；采用细菌分离培养法分离到3种形态不同的病原菌共计17株，其中革兰氏阳性短杆菌经鉴定为伪结核棒状杆菌，分离率57.9%（11/19），革兰氏阳性球菌经鉴定为金黄色葡萄球菌，分离率为21.1%（4/19），革兰氏阳性杆菌经鉴定为化脓隐秘杆菌，分离率为10.5%（2/19），PCR鉴定较细菌分离培养法灵敏度更高。3种病原菌对丁胺卡那、氟苯尼考等多种药物敏感。结果表明，伪结核棒状杆菌是引起该地区山羊皮下脓肿的主要病原，金黄色葡萄球菌和化脓隐秘杆菌同样可引起山羊皮下脓肿，特异性PCR方法较细菌分离培养更为高效、准确。本研究结果可为后续进一步分析其生物学特性，以及四川地区山羊皮下脓肿的综合性防制提供依据。

关键词: 山羊; 皮下脓肿; 分离鉴定; 伪结核棒状杆菌; 金黄色葡萄球菌; 化脓隐秘杆菌

Abstract:

To identify the main pathogens of goat subcutaneous abscess,a total of 19 abscess samples were collected from three goat farms in Lezhi county,Jintang county and Pengxi county in Sichuan province.The pathogens were detected and identified by bacterial isolation,biochemical test and specific PCR methods,and the drug sensitivity of the pathogens isolated was determined.The results showed that a total of 19 strains of 3 pathogens were isolated by PCR amplification method,and the isolation rate of Corynebacterium pseudotuberculos,Staphylococcus aureus and Arcanobacterium pyogenes was 57.9%(11/19),26.3% (5/19) and 15.8% (3/19),respectively.A total of 17 strains of 3 pathogens were isolated by isolation and identification method,in which 11 strains of Gram-positive Brevibacterium were identified as Corynebacterium pseudotuberculosis whose isolation rate was 57.9%(11/19),4 strains of Gram-positive cocci were identified as Staphylococcus aureus,and isolation rate was 21.1% (4/19),while 2 strains of Gram-positive bacilli were identified as Arcanobacterium pyogenes whose isolation rate was 10.5% (2/19).PCR was more sensitive than bacterial isolation.Three pathogens were sensitive to many drugs such as amikacin and florfenicol,etc.The results of this study provided a useful basis for further analysis of its biological characteristics and the development of comprehensive control measures for subcutaneous abscess in these areas.

Key words: goat; subcutaneous abscess; isolation and identification; Corynebacterium pseudotuberculosis; Staphylococcus aureus; Arcanobacterium pyogenes

中图分类号:

S852.6

张斯旂, 刀筱芳, 李垚, 马媛, 杨发龙. 山羊皮下脓肿的病原分离与鉴定[J]. 《中国畜牧兽医》, 2019, 46(3): 949-956.

ZHANG Siqi, DAO Xiaofang, LI Yao, MA Yuan, YANG Falong. Isolation and Identification of Etiological Bacteria of Subcutaneous Abscess in Goats[J]. , 2019, 46(3): 949-956.

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