牦牛泌乳期垂体组织比较转录组学分析

doi:10.16431/j.cnki.1671-7236.2019.03.022

《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (3): 824-831.doi: 10.16431/j.cnki.1671-7236.2019.03.022

牦牛泌乳期垂体组织比较转录组学分析

朱育星¹, 刘俊², 艾翳³, 陈通¹, 邓志和¹, 何世明⁴, 吴锦波⁴, 兰道亮³

1. 西南民族大学生命科学与技术学院, 成都 610041;
2. 辽宁省动物卫生监督所, 沈阳 110015;
3. 西南民族大学青藏高原研究院, 成都 610041;
4. 阿坝藏族羌族自治州畜牧科学技术研究所, 红原 624402

收稿日期:2018-11-06 出版日期:2019-03-20 发布日期:2019-03-20
通讯作者: 吴锦波, 兰道亮 E-mail:25974453@qq.com;landaoliang@163.com
作者简介:朱育星(1992-),女,四川西昌人,硕士生,研究方向:高原动物功能基因组,E-mail:549850163@qq.com
基金资助:
四川省科技厅2015年科技支撑计划项目（2015FZ0024）；西南民族大学研究生创新型项目（CX2018SZ33）；四川省科技厅应用基础项目（2016JY0024）；青藏高原生态畜牧业协同创新中心开放基金

Comparative Transcriptome Analysis of Lactation Pituitary Gland in Yak

ZHU Yuxing¹, LIU Jun², AI Yi³, CHEN Tong¹, DENG Zhihe¹, HE Shiming⁴, WU Jinbo⁴, LAN Daoliang³

1. College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China;
2. Liaoning Animal Health Supervision Institute, Shenyang 110015, China;
3. Institute of Qinghai-Tibetan Plateau, Southwest Minzu University, Chengdu 610041, China;
4. Aba Tibetan and Qiang Autonomous Prefecture Institute of Animal Science and Technology, Hongyuan 624402, China

Received:2018-11-06 Online:2019-03-20 Published:2019-03-20

摘要/Abstract

摘要：

试验旨在了解不同产奶量牦牛泌乳期垂体组织间的转录组差异，为进一步阐述牦牛垂体组织调控泌乳的机制提供参考。提取牦牛垂体组织总RNA，并应用RNA-Seq技术对高产奶量牦牛和低产奶量牦牛泌乳期间垂体组织转录组进行高通量测序及分析。结果显示，通过比较分析高产奶量牦牛和低产奶量牦牛垂体组织转录组数据，筛选出114个差异表达基因，其中55个表现为上调，59个表现为下调。进一步功能分析表明，这些差异基因涉及多种GO分类及KEGG通路，其中GO分析显示，差异基因与许多和氨基酸代谢相关的生物学过程存在紧密关联；KEGG通路分析显示，最为富集的是细胞黏附分子通路，且金黄色葡萄球菌感染和利什曼病等大量与免疫或疾病相关的通路也出现显著富集。本研究对比了高产奶量牦牛和低产奶量牦牛泌乳期垂体组织转录组数据，筛选并分析了相关差异表达基因，为提高牦牛产奶量的研究提供新的思路。

关键词: 牦牛; 垂体组织; 转录组; 比较分析; 分子机制

Abstract:

This study was aimed to explore the transcriptome difference of pituitary gland in yak with different milk production during lactation,and provide a reference for further elucidating the mechanism of lactation pituitary gland in yak.The total RNA was extracted from pituitary gland tissue,high-throughput sequencing and analysis of pituitary tissue transcriptome during high lactation calf and low-yield calf lactation were performed using RNA-Seq technology.By the comparison and analysis of the transcriptome data for the pituitary gland tissue of high-yielding yak and low-yielding yak,114 differential genes were screened out,of which 55 were up-regulated and 59 were down-regulated.Further functional analysis indicated that these differential expressed genes involved in multiple classes based on GO analysis and KEGG pathways.The GO analysis result showed that the functions of the differential genes were closely related to the amino acids metabolism and other biological processes.KEGG pathway analysis result revealed that these genes were mostly enriched in the pathway of cell adhesion molecule pathway.Moreover,several other pathways related to immune such as Staphylococcus aureus infection and leishmaniasis also showed significant enrichment.The pituitary gland transcriptome of high-yielding yak and low-yielding yak during lactation had been compared,and the related differentially expressed genes had been screened and analyzed,which provided new ideas for improving the milk production of yaks.

Key words: yak; pituitary gland; transcriptome; comparative analysis; molecule mechanism

中图分类号:

S814.8

朱育星, 刘俊, 艾翳, 陈通, 邓志和, 何世明, 吴锦波, 兰道亮. 牦牛泌乳期垂体组织比较转录组学分析[J]. 《中国畜牧兽医》, 2019, 46(3): 824-831.

ZHU Yuxing, LIU Jun, AI Yi, CHEN Tong, DENG Zhihe, HE Shiming, WU Jinbo, LAN Daoliang. Comparative Transcriptome Analysis of Lactation Pituitary Gland in Yak[J]. , 2019, 46(3): 824-831.

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牦牛泌乳期垂体组织比较转录组学分析

Comparative Transcriptome Analysis of Lactation Pituitary Gland in Yak

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