猪PRLR和FSHβ基因多态性检测及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2019.03.016

摘要/Abstract

摘要：

试验旨在研究催乳素受体（prolactin receptor，PRLR）和卵泡刺激素β（follicle-stimulating hormone β，FSHβ）基因的多态性及其与母猪繁殖性状的关联分析。采用PCR-RFLP方法对大白猪、杜洛克猪和长白猪3个猪种共487头母猪进行了PRLR和FSHβ基因多态性检验，并采用单因子方差分析、LSD法分析不同基因型与母猪总产仔数、产活仔数、断奶窝重和断奶窝仔数等繁殖性状的相关性。结果表明，PRLR基因在大白猪和长白猪中B等位基因均为优势基因，基因频率分别为0.717和0.548，大白猪BB基因型总产仔数显著高于AA基因型（P<0.05），长白猪BB基因型断奶窝重显著高于AB基因型（P<0.05）；FSHβ基因在大白猪、杜洛克猪和长白猪3个猪种中B等位基因均为优势基因，基因频率分别为0.804、0.760和0.789，长白猪BB基因型总产仔数和产活仔数均显著高于AB基因型（P<0.05），呈现BB>AA>AB趋势。因此，PRLR和FSHβ基因对荣昌猪场母猪繁殖性能有一定影响，可作为母猪繁殖性能分子选育的候选参考基因。

关键词: 猪; PRLR基因; FSHβ基因; 多态性; 繁殖性状

Abstract:

This study was aimed to investigate the polymorphism of prolactin receptor (PRLR) and follicle-stimulating hormone β (FSHβ) genes and its association with the reproductive traits in sows.The polymorphism of PRLR and FSHβ genes in 487 sows of Large White,Duroc and Landrace pigs was detected by PCR-RFLP method,and the association between different genotypes and reproductive traits such as total litter size,litter size,weaning litter weight and weaning litter size were analyzed by One-Way ANOVA analysis and LSD method.The results showed that B allele of PRLR gene was predominant allele in Large White and Landrace pigs,and the gene frequency were 0.717 and 0.548,respectively.The total number born of BB genotype in Large White pig was significantly higher than that of AA genotype (P<0.05),the weaning litter weight of BB genotype in Landrace pig was significantly higher than that of AB genotype (P<0.05).B allele of FSHβ gene had a high frequency in Large White,Duroc and Landrace pigs,and the gene frequency were 0.804,0.760 and 0.789,respectively.The total number born and number born alive of BB genotype in Large White pig was significantly higher than that of AB genotype (P<0.05),displaying BB>AA>AB trend.In conclusion,the polymorphism of PRLR and FSHβ genes significantly affected the reproductive traits of sows in Rongchang pigs farm,and they could be considered as candidate selective markers for molecular breeding of sows.

Key words: pig; PRLR gene; FSHβ gene; polymorphism; reproductive traits

中图分类号:

S8135.3

范一萍, 王晓梅, 贾启涛, 李成勃, 梁小娟, 曹春伟, 周磊, 陶聪, 赵建国, 刘剑锋, 王彦芳. 猪PRLR和FSHβ基因多态性检测及其与繁殖性状的关联分析[J]. 《中国畜牧兽医》, 2019, 46(3): 774-781.

FAN Yiping, WANG Xiaomei, JIA Qitao, LI Chengbo, LIANG Xiaojuan, CAO Chunwei, ZHOU Lei, TAO Cong, ZHAO Jianguo, LIU Jianfeng, WANG Yanfang. Polymorphism of PRLR and FSHβ Genes and Its Association Analysis with Reproductive Traits in Pigs[J]. , 2019, 46(3): 774-781.

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