成纤维细胞生长因子21及其受体1、2在小鼠毛囊形成期的定位和表达

doi:10.16431/j.cnki.1671-7236.2019.01.003

《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (1): 20-27.doi: 10.16431/j.cnki.1671-7236.2019.01.003

成纤维细胞生长因子21及其受体1、2在小鼠毛囊形成期的定位和表达

陆娜, 曹校瑞, 吴晋强, 闫瑞琴, 张娇娇, 吴佳豪, 赫晓燕

山西农业大学动物科技学院, 太谷 030801

收稿日期:2018-06-11 出版日期:2019-01-20 发布日期:2019-01-19
通讯作者: 赫晓燕 E-mail:sxndhxy@163.com
作者简介:陆娜(1993-),女,山西大同人,硕士生,研究方向:基础兽医学,E-mail:18234208420@163.com
基金资助:
山西省自然科学基金（201701D121106）

Localization and Expression of FGF21,FGFR1 and FGFR2 in Mice Hair Follicle Formation

LU Na, CAO Xiaorui, WU Jinqiang, YAN Ruiqin, ZHANG Jiaojiao, WU Jiahao, HE Xiaoyan

College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China

Received:2018-06-11 Online:2019-01-20 Published:2019-01-19

摘要/Abstract

摘要：

本试验旨在通过研究成纤维细胞生长因子21（fibroblast growth factor 21，FGF21）及其受体FGFR1、FGFR2在胚胎小鼠毛囊形成阶段中的定位与表达，从而探究其在小鼠毛囊形成中的作用。试验采用免疫组织化学、实时荧光定量PCR及Western blotting方法研究FGF21、FGFR1、FGFR2蛋白和mRNA在胚胎期13~18 d（E13~E18）小鼠皮肤中的表达。结果显示：①FGF21蛋白在E13主要表达于表层细胞、基底层细胞及结缔组织中，E14表达于表层细胞和基板，E15在毛芽中有微量表达，E16、E17表达于毛钉中，E18主要表达于未成熟毛囊细胞；FGFR1和FGFR2蛋白在E13~E18于表层细胞、基底层、基板、毛芽、毛钉、未成熟毛囊和结缔组织中均有表达。②实时荧光定量PCR及Western blotting结果显示：FGF21 mRNA和蛋白在E14相对表达量最高；FGFR1 mRNA及蛋白在E16~E17相对表达量较高；FGFR2 mRNA及蛋白的相对表达量在E13至E18均呈上升趋势，在E18相对表达量最高。结果提示：在小鼠胚胎期毛囊形成和发育过程中，FGF21可能在诱导毛囊启动过程中发挥一定的作用；FGFR1可能促进毛钉形成；FGFR2可能在毛囊形成和成熟中发挥重要作用。

关键词: 成纤维细胞生长因子21(FGF21); FGFR1; FGFR2; 小鼠胚胎; 毛囊形成期

Abstract:

This study had investigated the localization and expression of fibroblast growth factor 21 (FGF21) with its receptors FGFR1 and FGFR2 in the formation phase of embryonic mice hair follicles.E13-E18 embryonic mice back skin were used to determine the mRNA and protein relative expression levels of FGF21,FGFR1 and FGFR2 by immunohistochemistry,Real-time PCR and Western blotting analysis.The results showed that:①FGF21 expressed in epidermis,basal layer and connective tissue at E13;Expressed in epidermis and placode at E14;Slightly expressed in hair bud at E15;Expressed in hair peg at E16 and E17;Mainly expressed in immature hair follicle at E18.FGFR1 and FGFR2 expressed in epidermis,basal layer,placode,hair bud,hair peg,immature hair follicle and connective tissue at E13-E18.②The results of Real-time PCR and Western blotting showed that the relative expression levels of FGF21 mRNA and protein were higher at E14 than that of any other stages;The relative expression levels of FGFR1 mRNA and protein were higher at E16-E17;And the relative expression of FGFR2 mRNA and protein showed ascending trend from E13 to E18 and was highest at E18.In summary,during the embryonic hair follicle formation and development,FGF21 might play a role in inducing hair follicle activation;FGFR1 might promote hair peg formation;FGFR2 might be necessary to induce hair follicle formation and hair follicle maturation.

Key words: FGF21; FGFR1; FGFR2; embryonic mice; hair follicle formation stage

中图分类号:

Q954

陆娜, 曹校瑞, 吴晋强, 闫瑞琴, 张娇娇, 吴佳豪, 赫晓燕. 成纤维细胞生长因子21及其受体1、2在小鼠毛囊形成期的定位和表达[J]. 《中国畜牧兽医》, 2019, 46(1): 20-27.

LU Na, CAO Xiaorui, WU Jinqiang, YAN Ruiqin, ZHANG Jiaojiao, WU Jiahao, HE Xiaoyan. Localization and Expression of FGF21,FGFR1 and FGFR2 in Mice Hair Follicle Formation[J]. , 2019, 46(1): 20-27.

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成纤维细胞生长因子21及其受体1、2在小鼠毛囊形成期的定位和表达

Localization and Expression of FGF21,FGFR1 and FGFR2 in Mice Hair Follicle Formation

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