中药与抗菌药物联用对猪源大肠杆菌耐药性的抑制作用

doi:10.16431/j.cnki.1671-7236.2018.09.036

摘要/Abstract

摘要：

为了探讨中药与抗菌药物联用对大肠杆菌耐药性的作用效果，本研究对河南部分地区采集的样品（50份）进行分离鉴定、生化试验及致病性试验，对分离鉴定后的致病性猪大肠杆菌选用12种中药采用K-B法进行耐药性检测，将筛选出来的中药与抗生素进行中西联用对致病性大肠杆菌做体外抑菌试验，并分析中西药联用对大肠杆菌的抑制作用。结果显示，采集的50份样品有23株菌符合大肠杆菌的分离培养特性和生化特性，且23株菌均为致病性大肠杆菌；通过耐药性检测，23株试验菌株对测试的中药均有不同程度的耐药性，其中试验菌株对甘草耐药率最高（82.6%），其次为生地（78.2%），而对黄柏（17.3%）和白头翁（21.7%）耐药性较低，具有一定的敏感性；体外抑菌试验结果发现，中药与抗生素联用均有不同程度的抑菌效果，其中黄柏、白头翁与抗菌药物联用的抑菌效果最明显。以上结果说明中药与抗菌药物联用不仅可以抑制大肠杆菌的耐药性，还可以延缓大肠杆菌耐药性的产生。

关键词: 中药; 抗菌药物; 大肠杆菌; 耐药性; 抑制作用

Abstract:

This study was aimed to discuss the effect of drug resistance of Escherichia coli(E.coli) by combining traditional Chinese medicine and antibiotics drugs.50 samples which collected from Henan province were isolated for identification,biochemical experiments and pathogenicity test.12 kinds of traditional Chinese medicine were selected for isolation and identification of pathogenic porcine E.coli,and the K-B method was used to detect the drug resistance.The screened traditional Chinese medicines and antibiotics were used to conduct in vitro antibacterial tests against pathogenic E.coli,and analyze the inhibitory effect of Chinese and Western drugs on E.coli.The results showed that 23 strains of 50 samples were consistent with the isolation and culture characteristics and biochemical characteristics of E.coli.23 strains of bacteria were pathogenic Escherichia coli.Through the drug resistance test,23 test strains had different degrees of resistance to the tested traditional Chinese medicines.The resistance rate of the tested strains to licorice was the highest (82.6%),followed by the birthplace (78.2%),and the resistance to cork(17.3%) and pulsatilla (21.7%) were lower,with certain sensitivity.In vitro antibacterial result showed that the combination of traditional Chinese medicine and antibiotics had different degrees of antibacterial effect,and the antibacterial effect of cork,pulsatilla chinensis combined with antibiotics was the most obvious.It showed that the combination of traditional Chinese medicine and antibiotics could not only inhibit the drug resistance of E.coli,but also delay the emergence of drug resistance of E.coli.

Key words: traditional Chinese medicine; antibacterial drugs; Escherichia coli; drug resistance; inhibitory effect

中图分类号:

S852.61⁺2

张炳亮, 董发明, 王文文, 李鹏姗, 梁立钦. 中药与抗菌药物联用对猪源大肠杆菌耐药性的抑制作用[J]. 《中国畜牧兽医》, 2018, 45(9): 2628-2636.

ZHANG Bingliang, DONG Faming, WANG Wenwen, LI Pengshan, LIANG Liqin. Inhibition Effect of Drug Resistance of Swine Derived Escherichia coli by Combining Traditional Chinese Medicine and Antibacterial Drugs[J]. , 2018, 45(9): 2628-2636.

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