《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (8): 2175-2181.doi: 10.16431/j.cnki.1671-7236.2018.08.016

• 动物营养与饲料科学 • 上一篇    下一篇

L-茶氨酸对氧化应激断奶仔猪生长性能及血液生化指标的影响

闫峻1,2, 李泽青3, 王文杰1, 刘通4, 马衍旋4, 李宁1, 马墉1, 郑梓1, 穆淑琴1   

  1. 1. 天津市农业科学院, 天津市畜牧兽医研究所, 天津 300381;
    2. 天津大学化工学院, 天津 300381;
    3. 天津市畜牧兽医站, 天津 301800;
    4. 西北农林科技大学动物科技学院, 杨凌 712100
  • 收稿日期:2017-11-27 出版日期:2018-08-20 发布日期:2018-08-15
  • 通讯作者: 穆淑琴 E-mail:mushq@163.com
  • 作者简介:闫峻(1984-),男,江苏徐州人,博士,研究方向:单胃动物营养,E-mail:yjjsxz@163.com
  • 基金资助:

    天津市生猪产业技术体系创新团队(ITTPRS2017005);天津市农科院院长基金(15001);天津市科技支撑计划项目(15ZCZDNC00090)

Effects of L-theanine on Growth Performance and Blood Biochemical Indicators of Oxidative Stress Weaned Piglets

YAN Jun1,2, LI Zeqing3, WANG Wenjie1, LIU Tong4, MA Yanxuan4, LI Ning1, MA Yong1, ZHENG Zi1, MU Shuqin1   

  1. 1. Tianjin Institute of Animal Science and Veterinary, Tianjin Academy of Agricultural Science, Tianjin 300381, China;
    2. College of Chemical Engineering, Tianjin University, Tianjin 300072, China;
    3. Tianjin Animal Husbandry and Veterinary Station, Tianjin 301800, China;
    4. College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China
  • Received:2017-11-27 Online:2018-08-20 Published:2018-08-15

摘要:

本研究探讨了L-茶氨酸对氧化应激断奶仔猪生长性能、抗氧化能力及免疫功能的影响。试验采用2×2二因素设计,160头断奶仔猪(7.53 kg±0.51 kg)随机分为4个处理组:1组(日粮中不添加L-茶氨酸,腹腔注射5 mL生理盐水);2组(日粮中不添加L-茶氨酸,腹腔注射8 mg/kg体重敌快死);3组(日粮中添加1 000 mg/kg L-茶氨酸,腹腔注射5 mL生理盐水);4组(日粮中添加1 000 mg/kg L-茶氨酸,腹腔注射8 mg/kg体重敌快死)。预试期7 d,正试期28 d。结果显示:①与1组相比,2组仔猪平均日增重(ADG)和平均日采食量(ADFI)显著降低,而料重比显著升高(P<0.05);与2组相比,4组仔猪ADG显著增加(P<0.05)。②与1组相比,2组仔猪血清丙二醛(MDA)含量显著增加,而总抗氧化能力(T-AOC)和谷胱甘肽过氧化物酶(GSH-Px)活性显著下降(P<0.05),3组仔猪血清MDA显著降低,而T-AOC显著增加(P<0.05);与2组相比,4组仔猪血清MDA含量显著降低,而T-AOC和GSH-Px活性显著上升(P<0.05)。③与1组相比,2组仔猪血清IgA、IgG、IL-2、IL-4水平显著增加,C3和C4水平显著下降(P<0.05),3组仔猪血清IgA、IgG和IL-4水平显著降低,而C3和C4水平显著增加(P<0.05);与2组相比,4组仔猪血清IgA、IgG、IL-2、IL-4水平显著降低,而血清C3水平显著增加(P<0.05)。结果表明,注射敌快死可引发仔猪氧化应激,造成生长性能下降、体液免疫能力下降;日粮中添加1 000 mg/kg L-茶氨酸可缓解仔猪氧化损伤,提高氧化应激仔猪的生长性能、抗氧化能力和体液免疫能力。

关键词: L-茶氨酸; 断奶仔猪; 氧化应激; 生长性能; 体液免疫

Abstract:

To explore the effects of L-theanine on growth performance,antioxidant and immune response of oxidative stress weaned pigs,a two-factor (2×2) experimental design was employed.160 weaned pigs (7.53 kg±0.51 kg) were divided into 4 treatment groups:Treatment 1 (without L-theanine,intraperitoneal injection of 5 mL normal saline),treatment 2(without L-theanine,intraperitoneal injection of 8 mg/(kg·BW) diquat),treatment 3(diet added 1 000 mg/kg L-theanine,intraperitoneal injection of 5 mL normal saline) and treatment 4 (diet added 1 000 mg/kg L-theanine,intraperitoneal injection of 8 mg/(kg·BW) diquat).7 d for preliminary feeding and 28 d for formal feeding.The results showed that:① Compared to treatment 1,the average daily gain (ADG) and average daily feed intake (ADFI) of weaned pigs in treatment 2 decreased significantly (P<0.05),while F/G increased significantly (P<0.05);Compared to treatment 2,L-theanine significantly increased ADG and ADFI in treatment 4 (P<0.05).②Compared to treatment 1,diquat significantly increased serum malondialdehyde (MDA) content,while the total antioxidant capacity (T-AOC) and the activity of glutathione peroxidase (GSH-Px) significantly decreased in treatment 2 (P<0.05),serum MDA content in treatment 3 significantly increased while the activity of GSH-Px significantly tended down (P<0.05);Compared to treatment 2,L-theanine significantly decreased serum MDA content while increased the activity of T-AOC and GSH-Px in treatment 4 (P<0.05).③Compared to treatment 1,the levels of serum IgA,IgG and IL-2,IL-4 significantly increased,while C3 and C4 significantly decreased (P<0.05) in treatment 2,and the levels of serum IgA,IgG and IL-4 were significantly decreased,while the concentration of C3 and C4 significantly increased in treatment 3 (P<0.05);Compared to treatment 2,the levels of serum IgA,IgG and IL-2,IL-4 significantly decreased,while C3 significantly increased in treatment 4 (P<0.05).In conclusion,injection of diquat could cause oxidative stress in piglets,resulting decrease of growth performance and humoral immunity;Diets added 1 000 mg/kg L-theanine could relieve oxidative damage,improve the growth performance,the capacity of antioxidation and humoral immunity in oxidative stressed piglets.

Key words: L-theanine; weaned piglet; oxidative stress; growth performance; humoral immunity

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