新疆北疆地区猪源粪肠球菌的耐药性分析

doi:10.16431/j.cnki.1671-7236.2018.05.031

《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (5): 1374-1381.doi: 10.16431/j.cnki.1671-7236.2018.05.031

新疆北疆地区猪源粪肠球菌的耐药性分析

王蒙蒙¹, 张子荣², 欧都·吾吐那生³, 蒲敬伟⁴, 袁立岗⁴, 齐亚银¹

1. 石河子大学动物科技学院, 石河子 832000;
2. 第六师五家渠市畜牧兽医工作站, 五家渠 931300;
3. 新疆塔城地区畜牧科技研究推广中心, 塔城 834700;
4. 新疆兵团农十二师畜牧兽医工作站, 乌鲁木齐 830009

收稿日期:2017-10-23 出版日期:2018-05-20 发布日期:2018-05-19
通讯作者: 齐亚银 E-mail:qiyayin@163.com
作者简介:王蒙蒙(1991-),女,河北沧州人,硕士,研究方向:动物传染病诊断与防治,E-mail:1771297530@qq.com
基金资助:
国家自然科学基金项目“致幼畜脑膜炎粪肠球菌穿越血脑屏障的机制初探”（31760737）；国家自然科学基金项目“毒力因子参与不同源肠球菌的机会感染机制及整合子介导的耐药机制初探”（31260610）；兵团现代农业科技攻关与成果转化计划项目“生猪健康养殖及安全猪肉生产关键技术集成与示范”（2015AC026）

Drug Resistance Analysis of Swine Enterococcus faecalis in Northern Xinjiang

WANG Mengmeng¹, ZHANG Zirong², OU DU·Wu tu na sheng³, PU Jingwei⁴, YUAN Ligang⁴, QI Yayin¹

1. College of Animal Science & Technology, Shihezi University, Shihezi 832000, China;
2. Animal and Veterinary Workstation in Wu Jia Qu of the Sixth Division, Wujiaqu 931300, China;
3. Livestock Science and Technology Research and Extension Center of Tacheng Area, Tacheng 834700, China;
4. Animal and Veterinary Workstation in the Twelfth Division of Xinjiang Corps, Urumqi 830009, China

Received:2017-10-23 Online:2018-05-20 Published:2018-05-19

摘要/Abstract

摘要：

为了解新疆北疆地区猪源粪肠球菌的耐药性及相关耐药基因型的分布情况，本试验采用K-B（Kirby-Baller）琼脂扩散法检测了49株猪源粪肠球菌对8种抗菌药物的敏感性，并采用PCR法对9种相关耐药基因进行检测并测序，测序结果与GenBank中的相应基因序列比对。药敏试验结果显示，分离菌对链霉素耐药率最高，其次为青霉素和红霉素，对呋喃妥因、氨苄西林高度敏感。PCR检测结果显示，β-内酰胺类耐药基因tem的检出率最高，为93.88%，其次是四环素类耐药基因tetM，为85.71%，喹诺酮类基因gyrA和parC检出率均为42.86%，氨基糖苷类耐药基因aph（3'）-Ⅲ、aac（6'）/aph2″和ant（6'）-Ⅰ的检出率分别为36.73%、16.33%和16.33%，未检出mefA和ermB基因。本试验从表型与基因型分析发现，北疆地区猪源粪肠球菌的多重耐药现象非常严重，且其耐药表型与基因型并不完全一致。

关键词: 粪肠球菌; 抗菌药物; 耐药基因

Abstract:

In order to determine the drug resistance of swine Enterococcus faecalis and the distribution of genotypes resistance in Northern Xinjiang,total 49 isolates were evaluated for their sensitivity to 8 antimicrobial drugs by Kirby-Baller disc diffusion method,and 9 relative resistance genes were detected by PCR method.The amplified fragments were sequenced and compared with the corresponding gene sequences in GenBank.Susceptibility test results showed that the isolates had the highest resistance rate to streptomycin,followed by penicillin and erythromycin,which were highly sensitive to nitrofurantoin and ampicillin.PCR detection results showed that the detection rate of β-lactam resistance gene tem was the highest (93.88%),followed by the tetracycline resistance gene tetM (85.71%),the detection rates of quinolones resistance gene gyrA and parC both were 42.86%,the detection rates of aminoglycoside resistance genes aph(3')-Ⅲ,aac(6')/aph2″ and ant(6')-Ⅰ were 36.73%,16.33% and 16.33%,respectively,and no mefA and ermB genes were detected.The results showed that the multidrug resistance of swine Enterococcus faecalis in Northern Xinjiang was very serious,and its resistance phenotype was not consistent with the genotype.

Key words: Enterococcus faecalis; antibacterials; drug resistance gene

中图分类号:

S852.61

王蒙蒙, 张子荣, 欧都·吾吐那生, 蒲敬伟, 袁立岗, 齐亚银. 新疆北疆地区猪源粪肠球菌的耐药性分析[J]. 《中国畜牧兽医》, 2018, 45(5): 1374-1381.

WANG Mengmeng, ZHANG Zirong, OU DU·Wu tu na sheng, PU Jingwei, YUAN Ligang, QI Yayin. Drug Resistance Analysis of Swine Enterococcus faecalis in Northern Xinjiang[J]. , 2018, 45(5): 1374-1381.

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新疆北疆地区猪源粪肠球菌的耐药性分析

Drug Resistance Analysis of Swine Enterococcus faecalis in Northern Xinjiang

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