巨型玫瑰冠鸡H-FABP、A-FABP基因表达的发育性变化及其肌内脂肪含量研究

doi:10.16431/j.cnki.1671-7236.2018.05.021

摘要/Abstract

摘要：

为探讨心脏型脂肪酸结合蛋白（heart-type fatty acid-binding protein，H-FABP）和脂肪型脂肪酸结合蛋白（adipocyte fatty acid-binding protein，A-FABP）基因与巨型玫瑰冠鸡生长发育及肌内脂肪含量（IMF）的关系，研究H-FABP、A-FABP基因对巨型玫瑰冠鸡IMF含量和腹脂沉积的作用机制，本试验采集了巨型玫瑰冠鸡与良凤花鸡在不同周龄（2、4、6、8、10、12周龄）的腹脂、心肌、胸肌、腿肌组织样品共480份，采用实时荧光定量PCR对巨型玫瑰冠鸡与良凤花鸡不同生长阶段组织中H-FABP、A-FABP基因mRNA表达量进行了检测，并采用索氏浸提法测定了两种鸡12周龄的胸肌与腿肌的IMF含量。结果表明，巨型玫瑰冠鸡与良凤花鸡的H-FABP、A-FABP基因mRNA在腹脂、心肌、胸肌和腿肌组织中均有不同程度的表达，且两种鸡H-FABP基因mRNA在心肌组织中高度表达，在脂肪组织中表达量较低，在胸肌、腿肌组织中中度表达，而A-FABP基因相反，推测H-FABP基因主要在肌肉组织中表达，而A-FABP基因主要在脂肪组织中表达。良凤花鸡生长速度高于巨型玫瑰冠鸡；巨型玫瑰冠鸡的腹脂率均低于同性别的良凤花鸡，但胸肌、腿肌的IMF均高于同性别的良凤花鸡，表明巨型玫瑰冠鸡的肉品质优于良凤花鸡。本试验结果为巨型玫瑰冠鸡H-FABP、A-FABP基因分子选育奠定了基础。

关键词: 巨型玫瑰冠鸡; 良凤花鸡; H-FABP基因; A-FABP基因; 肌内脂肪

Abstract:

In order to investigate the relationship between heart-type fatty acid-binding protein (H-FABP) and adipocyte fatty acid-binding protein (A-FABP) genes and the growth and intramuscular fat (IMF) content of Rose cockscomb chicken,and the mechanism of H-FABP and A-FABP genes effects on the IMF content and abdominal fat deposition,a total of 480 copies of the abdominal fat, myocardium,chest muscle and leg muscle samples of Rose cockscomb and Liangfenghua chickens were collected,the H-FABP and A-FABP genes mRNA expression were analyzed by Real-time quantitative PCR,and the IMF content of chest and leg muscles of 12-week-old chickens was determined by Soxhlet extraction method.The results showed that the H-FABP and A-FABP genes were all expressed in abdominal fat, myocardium,chest muscle and leg muscle tissues with varying degrees.The H-FABP genes of Rose cockscomb and Liangfenghua chickens was highly expressed in myocardium tissues,moderately expressed in chest and leg muscles tissues,and rarely expressed in abdominal fat tissues.And the expression of A-FABP gene was on the contrary,indicating that H-FABP gene was mainly expressed in muscle tissues while A-FABP gene was mainly expressed in adipose tissue.Liangfenghua chicken grew faster than Rose cockscomb chicken,the abdominal fat rat of Rose cockscomb chicken were lower than single-sex Liangfenghua chicken,but the IMF in chest muscle and leg muscle were higher than that of single-sex Liangfenghua chicken,showing that the meat quality of Rose cockscomb chicken was better.The test result laid a solid foundation for the H-FABP and A-FABP genes molecular breeding of Rose cockscomb chicken.

Key words: Rose cockscomb chicken; Lianfenghua chicken; H-FABP gene; A-FABP gene; IMF

中图分类号:

S813.2

周志楠, 毛海彭, 丁秋芸, 吴昌炽, 冯振华, 张梅, 张苗苗, 孙杰, 廖和荣. 巨型玫瑰冠鸡H-FABP、A-FABP基因表达的发育性变化及其肌内脂肪含量研究[J]. 《中国畜牧兽医》, 2018, 45(5): 1289-1296.

ZHOU Zhinan, MAO Haipeng, DING Qiuyun, WU Changchi, FENG Zhenhua, ZHANG Mei, ZHANG Miaomiao, SUN Jie, LIAO Herong. The Developmental Changes of H-FABP and A-FABP Genes Expression and Intramuscular Fat Content of Rose Cockscomb Chicken[J]. , 2018, 45(5): 1289-1296.

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