某定点肉牛屠宰场中非O157致病性STEC的分离鉴定

doi:10.16431/j.cnki.1671-7236.2018.04.025

摘要/Abstract

摘要：

为了了解新疆伊犁地区肉牛屠宰过程中大肠杆菌的污染情况，检测非O157致病性产志贺毒素大肠杆菌（Shiga toxin-producing Escherichia coli，STEC）的感染情况，本试验采集新疆伊犁地区某定点肉牛屠宰场中屠宰肉牛的粪样和屠宰后的胴体表面拭子，并对样品进行了大肠杆菌的分离鉴定、毒力基因（eae、stx1、stx2）的PCR检测、O157鉴定（rfbE）、ERIC-PCR基因分型和小鼠致病性试验。结果显示，在采集的45份样品中分离鉴定出42株大肠杆菌，分离率为93.3%。其中2株菌株同时编码了毒力基因stx1和stx2，检出率为4.8%，毒力基因eae未被检出。PCR鉴定均为非O157 STEC。ERIC-PCR基因分型检测发现，2株菌的基因型非常相似，同源关系密切。对小鼠进行腹腔注射攻毒，攻菌6 h后，小鼠开始出现死亡，立即解剖死亡小鼠发现，其肠道出血，肝脏、脾脏、肾脏明显出血肿大，解剖对照小鼠表现正常，表明菌株具有一定的致病性。综上所述，在肉牛屠宰过程中存在大肠杆菌污染，其中粪便中非O157 STEC菌株对胴体造成了污染，需要加强控制肉牛的屠宰加工关键环节的环境卫生。

关键词: 非O157产志贺毒素大肠杆菌; 分离鉴定; 毒力基因; ERIC-PCR

Abstract:

This study was aimed to understand the contamination of Escherichia coli at the process of beef cattle slaughter in the region of Yili,Xinjiang,and to detect the infection of non-O157 pathogenic Shiga toxin-producing E.coli (STEC).The fecal samples of beef cattle and swab samples of carcass surface in a certain designated slaughterhouse in Yili were collected,and the samples were performed with an isolated identification of E.coli,PCR detection of virulence genes (eae,stx1 and stx2), O157 identification (rfbE),ERIC-PCR genotyping and mouse pathogenicity test.The results showed that 42 strains of E.coli were isolated and identified out from 45 samples,and the isolation rate was 93.3%.Among them, the virulence gene of eae was not detected,and two E.coli strains simultaneously programmed the virulence genes of stx1 and stx2,and the detection rate was 4.8%.All the identified strains were non-O157 type.Genotyping test of ERIC-PCR revealed that the two strains of non-O157 STEC were very similar and closely related to each other.The mice were injected with intraperitoneal injection,and started to die after 6 h of bacterial attack.It showed that there was a presence of intestinal hemorrhage,its liver,spleen and kidney were found to have obvious hemorrhage and enlargement,meanwhile the anatomical control mice showed no sign of anomaly,indicating that the strains had a certain pathogenicity.In conclusion,E.coli contamination was exist in the process of slaughtering of beef cattle.The non-O157 STEC in the faeces had contaminated the carcass,and the environmental health of key link of slaughter and processing of beef cattle should be strengthened.

Key words: non-O157 STEC; isolation and identification; virulence gene; ERIC-PCR

中图分类号:

S852.61

张妍, 刘英玉, 郑晓琴, 张超, 苏战强, 张晓红. 某定点肉牛屠宰场中非O157致病性STEC的分离鉴定[J]. 《中国畜牧兽医》, 2018, 45(4): 1035-1040.

ZHANG Yan, LIU Yingyu, ZHENG Xiaoqin, ZHANG Chao, SU Zhanqiang, ZHANG Xiaohong. Isolation and Identification of the Pathogenic Non-O157 STEC in Beef Slaughterhouse[J]. , 2018, 45(4): 1035-1040.

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