6个绵羊群体骨形态蛋白受体1B基因多态性分析

doi:10.16431/j.cnki.1671-7236.2018.04.017

摘要/Abstract

摘要：

试验旨在分析6个绵羊群体骨形态蛋白受体1B（bone morphogenetic protein receptor-1B，BMPR-1B）基因多态性，为绵羊繁殖力的标记辅助选择和育种提供理论依据。采集新吉细毛羊、中国美利奴羊（无角型）、东北细毛羊、杜×寒杂交羊（F1）、杜×寒杂交羊（F3）、白头杜泊羊6个绵羊群体共381只个体的血样，通过PCR-RFLP技术检测BMPR-1B基因多态性，并用DNAStar软件预测蛋白质二级结构，利用Excel 2003软件计算基因频率、基因型频率、Hardy-Weinberg平衡，以及遗传多样性参数（杂合度（He）、纯合度（Ho）、有效等位基因数（Ne）和多态信息含量（PIC））。结果显示，新吉细毛羊、东北细毛羊、中国美利奴羊（无角型）、白头杜泊羊BMPR-1B基因均不存在多态性；杜×寒杂交羊（F1）、杜×寒杂交羊（F3）具有多态性，存在3种基因型：++、B+和BB。杜×寒杂交羊（F1）χ²值达到显著水平，处于Hardy-Weinberg不平衡状态；杜×寒杂交羊（F3）χ²值未达到显著水平，处于Hardy-Weinberg平衡状态。杜×寒杂交羊（F1）、杜×寒杂交羊（F3）的PIC分别为0.311和0.264，为中度多态。推测BMPR-1B基因不是新吉细毛羊、东北细毛羊、中国美利奴羊（无角型）、白头杜泊羊4个绵羊群体多胎性状的主效基因，但可作为杜×寒杂交羊（F1）、杜×寒杂交羊（F3）多胎性状的主效基因。

关键词: 绵羊; BMPR-1B基因; 多态性

Abstract:

The study was aimed to analyze the gene polymorphism of bone morphogenetic protein receptor-1B (BMPR-1B) gene in six sheep population,which would provide a theoretical basis for marker-assisted selection and breeding for prolificacy in sheep.The blood samples of a total of 381 individuals from six sheep breeds (Xinji Fine wool sheep,Northeast Fine wool sheep,Chinese Merino (Mulley type) sheep,Dorper×Han crossbred sheep (F1),Dorper×Han crossbred sheep (F3) and White Dorper sheep) were used to study the polymorphism of BMPR-1B gene by PCR-RFLP.Secondary structures of proteins were predicted using DNAStar,gene frequency,genotype frequency,Hardy-Weinberg equilibrium and genetic diversity parameters (heterozygosity (He),homozygous (Ho),effective allele (Ne),polymorphic information content (PIC)) were calculated by Excel 2003 software.The results showed that there were no polymorphism in Xinji Fine wool sheep,Northeast Fine wool sheep,Chinese Merino (Mulley type) sheep and White Dorper sheep.The polymorphism in Dorper×Han crossbred sheep (F1) and Dorper×Han crossbred sheep (F3) existed three kind of genotypes (++,B+ and BB).According to χ² text,Dorper×Han crossbred sheep (F1) were not in Hardy-weinberg equilibrium,and Dorper×Han crossbred sheep (F3) were in Hardy-weinberg equilibrium.The PIC were 0.311 and 0.264 in the Dorper×Han crossbred sheep (F1) and Dorper×Han crossbred sheep (F3),which both meaned moderate diversity.The BMPR-1B gene might be a major gene which affected the prolificacy trait of Dorper×Han crossbred sheep (F1) and Dorper×Han crossbred sheep (F3).

Key words: sheep; BMPR-1B gene; polymorphism

中图分类号:

S813.3

吴翠玲, 宗兴龙, 赵卓, 赵云辉, 翟博, 张明新, 王春昕. 6个绵羊群体骨形态蛋白受体1B基因多态性分析[J]. 《中国畜牧兽医》, 2018, 45(4): 970-976.

WU Cuiling, ZONG Xinglong, ZHAO Zhuo, ZHAO Yunhui, ZHAI Bo, ZHANG Mingxin, WANG Chunxin. Genetic Polymorphism of BMPR-1B Gene in Six Sheep Breeds[J]. , 2018, 45(4): 970-976.

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