《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (2): 486-492.doi: 10.16431/j.cnki.1671-7236.2018.02.025

• 预防兽医 • 上一篇    下一篇

成都地区宠物犬感染犬瘟热病毒和犬呼吸道冠状病毒的分子流行病学调查

张昕, 黄坚, 张萍, 杨晓农   

  1. 西南民族大学生命科学与技术学院, 成都 610041
  • 收稿日期:2017-08-07 出版日期:2018-02-20 发布日期:2018-02-10
  • 通讯作者: 杨晓农 E-mail:yangxn058@163.com
  • 作者简介:张昕(1992-),女,辽宁丹东人,硕士生,研究方向:小动物疾病,E-mail:13679066206@163.com
  • 基金资助:

    四川省科技培训项目(2016KZ0007)

Molecular Epidemiologic Investigation on Infections of Canine Distemper Virus and Canine Respiratory Coronavirus in Pet Dogs in Chengdu Area

ZHANGX Xin, HUANG Jian, ZHANG Ping, YANG Xiaonong   

  1. College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China
  • Received:2017-08-07 Online:2018-02-20 Published:2018-02-10

摘要:

为了解成都地区宠物犬犬瘟热病毒(canine distemper virus,CDV)和犬呼吸道冠状病毒(canine respiratory coronavirus,CRCoV)的感染情况,本试验应用RT-PCR对采自成都地区8家动物医院共计420份出现呼吸道症状的宠物犬鼻腔棉拭子样本进行分子检测。结果发现,从420份样本中,检出213份CDV阳性,检出率为50.71%;检出247份CRCoV阳性,检出率为58.81%;CDV和CRCoV混合感染的检出率为41.19%。表明成都地区宠物犬感染CDV和CRCoV较为严重,且二者混合感染率较高。宠物犬CDV和CRCoV的检出率与年龄、性别、品种、季节和免疫状况等因素的关系存在不同程度的差异。其中,1~3月龄幼犬检出率最高,分别为74.40%和79.20%;纯种犬的检出率较其他犬种高,分别为59.37%和62.22%;春季CDV的检出率较高,为56.19%,而冬季CRCoV的检出率较高,为67.59%;未免疫犬的检出率较高,分别为64.42%和63.46%。该研究丰富了成都地区宠物犬CDV和CRCoV的流行病学资料,为该地区宠物犬CDV和CRCoV的诊断及防控提供了基本数据。

关键词: 犬传染性呼吸道疾病(CIRD); 犬瘟热病毒(CDV); 犬呼吸道冠状病毒(CRCoV); 分子流行病学

Abstract:

In order to investigate the prevalence and distribution of canine distemper virus (CDV) and canine respiratory coronavirus (CRCoV) infections in pet dogs in Chengdu area, 420 samples of nasal cotton swabs were collected from dogs with respiratory signs in eight animal hospitals in Chengdu area, and the molecular detection were carried out using the RT-PCR assay. From 420 samples, 213 samples were detected as CDV positive with the positive rate of 50.71%, and 247 samples were detected as CRCoV positive with the positive rate of 58.81%; The positive rate of mixed detection of CDV and CRCoV was 41.19%. The results indicated that the infection of CDV and CRCoV in pet dogs in Chengdu area was serious, and the mixed infection rate was also high. The detection rate of CDV and CRCoV in pet dogs was different from age, sex, variety, season and immunity. The detection rates of CDV and CRCoV in 1 to 3 month-old puppies were the highest, up to 74.40% and 79.20%, respectively; And in purebred dogs were 59.37% and 62.22%, respectively, which were higher than other varieties; The detection rate of CDV was higher up to 56.19% in spring, however the detection rate of CRCoV was higher up to 67.59% in winter. The detection rates of CDV and CRCoV in non-vaccinated dogs were higher than other dogs which were 64.42% and 63.46%, respectively. This study enriched the epidemiological data of CDV and CRCoV in pet dogs,and provided basic data for diagnosis and control of CDV and CRCoV infections in pet dogs in Chengdu area.

Key words: canine infectious respiratory disease (CIRD); canine distemper virus (CDV); canine respiratory coronavirus (CRCoV); molecular epidemiology

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