茶树油对断奶仔猪生长性能、血清生化指标及脏器指数的影响

doi:10.16431/j.cnki.1671-7236.2018.01.011

摘要/Abstract

摘要：

试验旨在研究日粮中添加不同剂量茶树油对断奶仔猪生长性能、腹泻情况、血清生化指标和脏器指数的影响，并探究其替代抗生素的可行性。试验选取21日龄、体重（6.73±0.12）kg的健康杜洛克×长白猪×大白猪三元杂交断奶仔猪120头，随机分为5组，分别为对照组（CON组，基础日粮）、抗生素组（ANT组，基础日粮+200 mg/kg 10%硫酸黏菌素+75 mg/kg 15%金霉素）、低剂量茶树油组（LTO组，基础日粮+50 mg/kg茶树油）、中剂量茶树油组（MTO组，基础日粮+100 mg/kg茶树油）和高剂量茶树油组（HTO组，基础日粮+150 mg/kg茶树油），每组6个重复，每个重复4头仔猪。正试期21 d。试验期间，测定每组仔猪的平均日增重（ADG）、平均日采食量（ADFI）及料重比（F/G）；在试验结束时，每个重复选取1头仔猪采血后屠宰，取心脏、肝脏、脾脏、肺脏、肾脏、胸腺、胰脏，测定脏器指数。结果显示：①MTO、HTO组ADG较CON组分别增加8.33%和14.84%（P>0.05），F/G分别降低9.58%和7.78%（P>0.05），二者均与ANT组无显著差异（P>0.05）；②与CON组相比，ANT、MTO和HTO组腹泻率和腹泻指数均显著降低（P<0.05），其中MTO组降幅最大；③与ANT组相比，MTO、HTO组谷草转氨酶（AST）及谷氨酰基转移酶（GGT）活性显著降低（P<0.05），LTO、HTO组葡萄糖（GLU）含量显著降低（P<0.05），各组血清中谷丙转氨酶（ALT）、总蛋白（TP）、白蛋白（ALB）、球蛋白（GLOB）和白蛋白/球蛋白（A/G）均无显著差异（P>0.05）；④与ANT组相比，MTO、HTO组的肝脏指数显著升高（P<0.05），LTO组胸腺指数显著升高（P<0.05）。综上所述，在日粮中添加适量茶树油（100 mg/kg）可一定程度促进断奶仔猪生长、降低腹泻，促进肝脏和胸腺的发育，可一定程度替代抗生素的使用。

关键词: 茶树油; 断奶仔猪; 生长性能; 腹泻率; 血清生化指标; 脏器指数

Abstract:

The experiment was carried out to investigate the effects of tea tree oil on growth performance,serum biochemical indexes and viscera indexes of weaned piglets,and discuss the possibility of tea tree oil replace the antibiotics. One hundred and twenty Duroc pig×Landrace pig×Yorkshire pig crossbreed weaned piglets (21-day-old,6.73 kg±0.12 kg) were randomly divided into five groups:CON group (basal diet),ANT group (basal diet+200 mg/kg colistin (10%)+75 mg/kg chlortetracycline (15%)),LTO group (basal diet+50 mg/kg tea tree oil),MTO group (basal diet+100 mg/kg tea tree oil) and HTO group (basal diet+150 mg/kg tea tree oil),with six replicates per group and four pigs per replicate. The experiment lasted for 21 d. During the experiment,the growth performance was measured (ADG,ADFI and F/G),and at the end of the experiment,one piglet was chosen from each replicate for blood sampling and then were slaughtered,the heart,liver,spleen,lung,kidney,thymus and pancreas were collected to determine the organ indexes.The results showed as follow:① Compared with CON group,ADG of MTO and HTO groups increased by 8.33% and 14.84% (P>0.05),respectively, F/G were decreased 9.58% and 7.78%,and the two indexes were not significant different with ANT group (P>0.05).②Diarrhea rate and diarrhea index of ANT,MTO and HTO groups were significantly lower than that of CON (P<0.05),in which MTO was the lowest.③Compared with ANT group,the activities of AST and GGT in MTO and HTO groups were significantly reduced (P<0.05),GLU in LTO and HTO groups were significantly decreased (P<0.05),there were no significant difference in ALT,TP,ALB,GLOB and A/G among groups (P>0.05).④The liver index in MTO and MTO groups and thymus index in LTO group were significantly higher than ANT group (P<0.05).In summary,adding a certain percentage of tea tree oil (100 mg/kg) to diet of weaned piglets could promote the growth performance,reduce diarrhea, promote the development of liver and thymus with a certain degree of regulate metabolism.

Key words: tea tree oil; weaned piglet; growth performance; diarrhea rate; serum biochemical indexes; organ indexes

中图分类号:

S816.7

王淑楠, 董丽, 魏臻武, 霍永久, 方伟, 封飞飞, 朱新宇, 喻礼怀. 茶树油对断奶仔猪生长性能、血清生化指标及脏器指数的影响[J]. 《中国畜牧兽医》, 2018, 45(1): 86-92.

WANG Shunan, DONG Li, WEI Zhenwu, HUO Yongjiu, FANG Wei, FENG Feifei, ZHU Xinyu, YU Lihuai. Effects of Tea Tree Oil on Growth Performance,Serum Biochemical and Viscera Indexes of Weaned Piglets[J]. , 2018, 45(1): 86-92.

参考文献

[1] FRANZ C,BASER K,WINDISCH W. Essential oils and aromatic plants in animal feeding a European perspective.A review[J].Flavour & Fragrance Journal,2010,25(5):327-340.
[2] MOUSSAOUI F,ALAOUI T. Evaluation of antibacterial activity and synergistic effect between antibiotic and the essential oils of some medicinal plants[J].Asian Pacific Journal of Tropical Biomedicine, 2016,6(1):32-37.
[3] 周选武, 杨开云,陈代文,等. 饲粮添加抗生素和植物精油对母猪生产性能、免疫功能和乳成分的影响[J].动物营养学报,2017,29(3):995-1002. ZHOU X W,YANG K Y,CHEN D W,et al. Effects of dietary supplementation of antibiotics and essential oils on sow production performance,immune function and milk composition[J].Journal of Animal Nutrition, 2017,29(3),995-1002.(in Chinese)
[4] HONG J C,STEINER T,AUFY A,et al.Effects of supplemental essential oil on growth performance,lipid metabolites and immunity,intestinal characteristics,microbiota and carcass traits in broilers[J]. Livestock Science,2012,144(3):253-262.
[5] GREAY S J,IRELAND D J,KISSICK H T,et al. Induction of necrosis and cell cycle arrest in murine cancer cell lines by Melaleuca alternifolia (tea tree) oil and terpinen-4-ol[J]. Cancer Chemotherapy and Pharmacology,2010,65(5):877-888.
[6] TIGHE S,GAO Y Y,TSENG S C,et al.Terpinen-4-ol is the most active ingredient of tea tree oil to kill demodex mites[J].Translational Vision Science & Technology,2013,2(7):2.
[7] 丁兆坤,高巍,许友卿,等. 茶树油对罗非鱼生长性能、脏器指数的影响[J].北京农业,2015,11:122-123. DING Z K,GAO W,XU Y Q,et al. Effects of tea tree oil on growth performance and organ indexes of tilapia[J].Beijing Agriculture,2015,11:122-123. (in Chinese)
[8] HUANG C H,QIAO S Y,LI D,et al. Effects of Lactobacilli on the performance,diarrhea incidence, VFA concentration and gastrointestinal microbial flora of weaning pigs[J]. Asian-Australasian Journal of Animal Sciences,2004,17(3):401-409.
[9] 刘正旭.竹酢粉对断奶仔猪生产性能、免疫机能及肠道微生物区系的影响[D].扬州:扬州大学,2016. LIU Z X. Effects of bamboo powder on production performance, immune function and intestinal microflora of weaned piglets[D]. Yangzhou:Yangzhou University,2016. (in Chinese)
[10] 刘猛.植物精油对仔猪生产性能、肠道微生物及免疫性能的影响[D].郑州:河南农业大学,2011. LIU M. Effects of plant essential oil on performance,intestinal microflora and immune performance of piglets[D].Zhengzhou:Henan Agricultural University,2011.(in Chinese)
[11] 方热军,李美君,周学彬,等. 植物精油提取物对断奶仔猪生产性能及血液生化指标的影响[J].饲料工业,2010, 31(16):9-12. FANG R J,LI M J,ZHOU X B,et al. Effects of extracts from plant essential oil on performance and blood biochemical indexes of weaned piglets[J].Feed Industry,2010,31(16):9-12.(in Chinese)
[12] 孙鎏国,杨海燕,陆玉娟,等.牛至油对断奶仔猪生产性能的影响[J].中国畜牧兽医文摘,2006,2:37. SUN L G,YANG H Y, LU Y J,et al. Effects of oregano oil on production performance of weaned piglets[J]. Chinese Abstract of Animal Husbandry and Veterinary Medcine,2006,2:37.(in Chinese)
[13] CARSON C F,HAMMER K A,RILEY T V. Melaleuca alternifolia oil:A review of antimicrobial and other medicinal properties[J].Clinical Microbiology Reviews,2006,19(1):50-62.
[14] HAMMER K A, CARSON C F.Effects of melaleuca alternifolia essential oil and the major monoterpene component terpinen-4-ol on the development of single-and multistep antibiotic resistance and antimicrobial susceptibility[J]. Antimicrobial Agents and Chemotherapy,2012,56(2):909-915.
[15] 方秋红,侯永清,陈振强,等.植物精油对断奶仔猪生长性能及血液生化指标的影响[J].饲料工业,2014,35(17):44-47. FANG Q H,HOU Y Q,CHEN Z Q,et al. Effects of plant essential oils on growth performance and blood biochemical indexes of weaned piglets[J]. Feed Industry,2014,35(17):44-47. (in Chinese)
[16] 王亮,梁代华,杨运玲,等.4种抗生素替代产品对断奶仔猪生长性能、血液生化及免疫性能的影响[J].饲料研究,2015,6:53-56. WANG L,LIANG D H,YANG Y L,et al. Effects of four kinds of antibiotic substitutes on growth performance, blood biochemistry and immune performance of weaned piglets[J].Feed Research,2015,6:53-56.(in Chinese)
[17] 张勇,王萌,李方方,等.三丁酸甘油酯和牛至油对断奶仔猪生长性能、血清生化指标和营养物质表观消化率的影响[J].动物营养学报,2016,28(9):2786-2794. ZHANG Y, WANG M, LI F F,et al. Effects of glyceryl tributyrate and origanum oil on growth performance, serum biochemical indexes and apparent digestibility of nutrients in weaned piglets[J]. Journal of Animal Nutrition,2016,28(9):2786-2794. (in Chinese)
[18] 邹思湘.动物生物化学[M].第4版.北京:中国农业出版社,2005. ZOU S X.Animal Biochemistry[M].4th edition. Beijing:China Agricultural Press,2005.(in Chinese)
[19] 史自涛,姚焰础,江山,等.粪肠球菌替代抗生素对断奶仔猪生长性能、腹泻率、血液生化指标和免疫器官的影响[J].动物营养学报,2015,27(6):1832-1840. SHI Z T,YAO Y C,JIANG S,et al. Effects of enterococcus faecalis substitute for antibiotics on growth performance, diarrhea rate,blood biochemical parameters and immune organs of weaned piglets[J]. Journal of Animal Nutrition,2015,27(6):1832-1840. (in Chinese)
[20] BALDISSERA M D,SOUZA C F,JÚNIOR G B,et al. Melaleuca alternifolia essential oil enhances the non-specific immune system and prevents oxidative damage in Rhamdia quelen experimentally infected by Aeromonas hydrophila:Effects on cholinergic and purinergic systems in liver tissue[J].Fish & Shellfish Immunology,2017,61:1-8.
[21] 禚梅,呙于明,赵小刚,等.日粮中添加植物提取物对肉仔鸡免疫机能的影响[J].今日畜牧兽医,2009,36(6):14-18. ZHOU M,GUO Y M,ZHAO X G,et al. The effect of different concentration of extracts of plant supplementation in diets on the growth performance and immune function in broilers[J]. Today's Animal Husbandry and Veterinary Medicine,2009,36(6):14-18. (in Chinese)