《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (10): 2944-2950.doi: 10.16431/j.cnki.1671-7236.2017.10.016

• 动物营养与饲料科学 • 上一篇    下一篇

复合菌发酵玉米蛋白粉的条件优化

魏炳栋1, 苗国伟2, 陈群1, 邱玉朗1, 李林1, 于维1, 李伟忠3   

  1. 1. 吉林省农业科学院畜牧分院, 公主岭 136100;
    2. 吉林农业大学, 长春 130033;
    3. 潍坊学院, 潍坊 261061
  • 收稿日期:2016-12-06 出版日期:2017-10-20 发布日期:2017-10-20
  • 通讯作者: 陈群 E-mail:chenqun96@163.com
  • 作者简介:魏炳栋(1981-),男,甘肃永靖人,博士,副研究员,研究方向:生物饲料,E-mail:weibingdong@foxmail.com
  • 基金资助:

    吉林省肉羊产业体系;吉林省科技厅项目(2013020638NY);潍坊学院青年科研基金(2012Z19)

Optimization of Fermentation Conditions for Corn Gluten Meal by Mixed Strains

WEI Bing-dong1, MIAO Guo-wei2, CHEN Qun1, QIU Yu-lang1, LI Lin1, YU Wei1, LI Wei-zhong3   

  1. 1. Branch Institute of Animal Science, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China;
    2. Jilin Agricultural University, Changchun 130033, China;
    3. Weifang University, Weifang 261061, China
  • Received:2016-12-06 Online:2017-10-20 Published:2017-10-20

摘要:

试验旨在研究复合菌(黑曲霉、乳酸菌、酵母菌和地衣芽孢杆菌)发酵玉米蛋白粉的优化条件。通过各菌种玉米蛋白粉水解驯化试验、菌种混合比例及发酵条件(温度、时间、含水量及初始pH)的正交试验,以玉米蛋白粉多肽得率为指标,确定发酵玉米蛋白粉的最优发酵条件。并在此条件下,测定发酵液中粗蛋白质、总糖、还原糖、氨基酸含量以及多肽得率。结果显示,正交试验优化的最佳接种比例为地衣芽孢杆菌4%、乳酸菌4%、酵母菌6%、黑曲霉6%,最佳发酵条件为发酵温度35℃、发酵时间84 h、含水量45%、初始pH为6,在此条件下多肽得率为44.03%。在发酵过程中,粗蛋白质含量和多肽得率呈上升趋势,发酵终止时,粗蛋白质含量提高了15.17%,多肽得率提高了205%,还原糖含量提高了850%,总必需氨基酸和总氨基酸含量分别增加了12.58%和12.37%。本试验结果可为扩大玉米蛋白粉的应用提供科学依据。

关键词: 复合菌; 玉米蛋白粉; 发酵; 条件优化

Abstract:

The objective of this experimentation was to optimizate the fermentation conditions for corn gluten meal by four different bacterial strains (Aspergillus niger,Lactobacillus,yeast and Bacillus licheniformis) through the acclimatization test of strains for hydrolysis corn gluten meal,orthogonal experimental of strains proportion determination and fermentation condition (temperature,time,moisture content and initial pH) and taking the peptides yield rate as an index.And then the crude protein,yield of peptides,total sugar,reducing sugar and amino acid constitute of corn gluten meal fermented at the optimal condition were determined. The results showed that the inoculation proportion of Bacillus licheniformis,Lactobacillus,yeast and Aspergillus niger were 4%,4%,6% and 6%,respectively. The optimal conditions for fermentation was that the fermentation temperature,fermentation time,moisture content and initial pH were 35℃,84 h,45% and 6,respectively,and the peptides yield rate was 44.03%.During fermentation,the crude protein content and peptides yield were increased.At the end of the fermentation,the content of crude protein,peptides yield rate,reducing sugar,total essential amino acid and total amino acid and were increased by 15.17%,205%,850%,12.58% and 12.37%,respectively.

Key words: mixed strains; corn gluten meal; fermentation; conditions optimization

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