《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (8): 2277-2282.doi: 10.16431/j.cnki.1671-7236.2017.08.009

• 生理生化 • 上一篇    下一篇

添加通路阻断剂对IGF-1影响奶牛乳腺上皮细胞增殖与凋亡的研究

雒诚龙, 赵艳坤, 武开乐, 邵伟   

  1. 新疆农业大学动物科学学院, 乌鲁木齐 830052
  • 收稿日期:2017-04-06 出版日期:2017-08-20 发布日期:2017-08-18
  • 通讯作者: 邵伟 E-mail:67696000@qq.com
  • 作者简介:雒诚龙(1993-),男,甘肃甘谷人,硕士生,研究方向:反刍动物营养,E-mail:422746985@qq.com
  • 基金资助:

    国家自然科学基金(31560645);新疆维吾尔自治区重点实验室开放课题(2015KL018);中国博士后基金委资助

Study on Effect of IGF-1 on Proliferation and Apoptosis of Bovine Mammary Epithelial Cells by Adding Inhibitor

LUO Cheng-long, ZHAO Yan-kun, WU Kai-le, SHAO Wei   

  1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
  • Received:2017-04-06 Online:2017-08-20 Published:2017-08-18

摘要:

试验旨在研究胰岛素样生长因子-1(insulin-like growth factor 1,IGF-1)对奶牛乳腺上皮细胞(bovine mammary epithelial cells,BMECs)增殖的影响,为后期利用IGF-1调控乳腺发育奠定理论基础。以奶牛乳腺上皮细胞为材料,首先分4组分别外源添加0(对照组)、10、50、100 μg/mL IGF-1且分别培养12、24、48、72 h,测定抑制BMECs凋亡率的最佳浓度;然后分6组:单纯BMECs组、BMECs+IGF-1组、BMECs+LY294002组、BMECs+IGF-1+LY294002组、BMECs+RAPA组和BMECs+IGF-1+RAPA组,采用流式细胞术测定各组细胞凋亡率。结果表明,外源性添加IGF-1对BMECs凋亡率具有抑制作用,最佳浓度为50 μg/mL;BMECs+IGF-1+LY294002与BMECs+IGF-1+RAPA组细胞凋亡率均极显著高于BMECs+IGF-1组(P<0.01)。推断IGF-1能够活化PI3K/Akt/mTOR信号通路,参与BMECs凋亡的调控作用,进而抑制BMECs细胞凋亡;IGF-1可能会对被抑制PI3K/Akt/mTOR信号通路产生"修复"机制,使其能够重新参与BMECs的生命进程。

关键词: 胰岛素样生长因子-1(IGF-1); 奶牛乳腺上皮细胞(BMECs); 增殖; 凋亡

Abstract:

The test was aimed to study the effect of insulin-like growth factor 1(IGF-1) on the proliferation of bovine mammary epithelial cells (BMECs),and provide a theoretical basis for the subsequent regulating mammary gland development using IGF-1. The optimal IGF-1 concentration for inhibiting BMECs apoptosis was obtained by measuring the apoptosis rate at 12, 24, 48 and 72 h after the addition of IGF-1 at four groups (0, 10, 50 and 100 μg/mL). Then divided into six groups:BMECs group, BMECs+IGF-1 group, BMECs+LY294002 group, BMECs+IGF-1+LY294002 group, BMECs+RAPA group and BMECs+IGF-1+RAPA group,and the apoptosis rates were detected by flow cytometry. The results showed that the heterologous IGF-1 had an inhibitory effect on the apoptosis of BMECs with an optimal concentration of 50 μg/mL. The apoptosis rates of BMECs+IGF-1+LY294002 and BMECs+IGF-1+RAPA groups were extremely significantly higher than BMECs+IGF-1 group (P<0.01). This study suggested that IGF-1 could activate the PI3K/Akt/mTOR signaling pathway and thereby inhibit the apoptosis of BMECs. Moreover, IGF-1 might also promote the "repair" mechanism for the inhibited PI3K/Akt/mTOR signaling pathway, and therefore make it reparticipate in BMECs life process.

Key words: insulin-like growth factor 1(IGF-1); bovine mammary epithelial cells (BMECs); proliferation; apoptosis

中图分类号: