胶质纤维酸性蛋白在黄羽肉鸡肠道中的分布研究

doi:10.16431/j.cnki.1671-7236.2017.05.009

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (5): 1315-1320.doi: 10.16431/j.cnki.1671-7236.2017.05.009

胶质纤维酸性蛋白在黄羽肉鸡肠道中的分布研究

张玉宇¹, 赵新林², 祁凤华¹, 陆艳¹, 石晓雨¹, 韩小丽¹, 徐春生¹

1. 石河子大学动物科技学院, 石河子 832003;
2. 新疆兵团科技局, 乌鲁木齐 830000

收稿日期:2016-07-17 出版日期:2017-05-20 发布日期:2017-05-18
通讯作者: 徐春生 E-mail:csxu2004@163.com
作者简介:张玉宇(1995-),女,山东济南人,本科,研究方向:动物比较组织学,E-mail:834715186@qq.com
基金资助:
国家自然科学基金资助项目（31560683）；石河子大学高层次人才科研启动资金专项（RCZX201105）

Study on the Distribution of Glial Fibrillary Acidic Protein in Intestine of Yellow Feather Broiler

ZHANG Yu-yu¹, ZHAO Xin-lin², QI Feng-hua¹, LU Yan¹, SHI Xiao-yu¹, HAN Xiao-li¹, XU Chun-sheng¹

1. College of Animal Science and Technology, Shihezi University, Shihezi 832003, China;
2. Bureau of Science and Technology, Corps xinjiang, Wulumuqi 830000, China

Received:2016-07-17 Online:2017-05-20 Published:2017-05-18

摘要/Abstract

摘要：

本研究旨在探究胶质纤维酸性蛋白（glial fibrillary acidic protein，GFAP）在黄羽肉鸡肠道中的分布规律。使用免疫组织化学SABC-AP法，观察鸡肠道中GFAP的分布规律。结果显示，GFAP在鸡小肠黏膜上皮、小肠腺细胞腔面、小肠黏膜下神经丛和肌间神经丛及其血管壁周围均呈强阳性表达，在黏膜固有膜上呈阳性表达；GFAP在鸡大肠黏膜上皮、大肠腺中呈阳性表达，在大肠黏膜下神经丛和肌间神经丛均呈强阳性表达。GFAP是肠神经胶质细胞的特异性标记物之一，观察其在鸡肠道的分布特征有助于阐明肠神经胶质细胞在肠道各段的分布规律，为研究鸡肠神经胶质细胞的功能提供形态学依据。

关键词: 黄羽肉鸡; 肠神经胶质细胞; 胶质纤维酸性蛋白; 小肠; 大肠; 免疫组织化学

Abstract:

The experiment was conducted to explore the distribution of glial fibrillary acidic protein (GFAP) in Yellow feather broiler,and to investigate the morphological characteristics of glial cells of chicken. The distribution of GFAP was studied by immunohistochemistry SABC-AP method. The results showed that GFAP were expressed strong positively in chicken small intestinal mucosa epithelium, intestinal gland cell cavity surface, submucosal plexus and myenteric plexus; The expressions of GFAP were positive in the mucosal lamina propria and myenteric nerve plexus around the blood vessel; In avian escherichia sticky epithelial membrane, colorectal adenocarcinoma,GFAP were expressed positively, and the expressions were strong positive in mucosa epithelium, submucosal plexus and myenteric plexus. GFAP was one of the specific marker of enteric glial cells, and the observation of distribution of GFAP in chicken intestinal tract was help for elucidating the enteric glial cells in the distribution of the intestine and providing the morphological basis for the study of chicken glial cell function.

Key words: Yellow feather broiler; enteric glial cells; GFAP; large intestine; small intestine; immunohistochemistry

中图分类号:

S852.16⁺2

张玉宇, 赵新林, 祁凤华, 陆艳, 石晓雨, 韩小丽, 徐春生. 胶质纤维酸性蛋白在黄羽肉鸡肠道中的分布研究[J]. 《中国畜牧兽医》, 2017, 44(5): 1315-1320.

ZHANG Yu-yu, ZHAO Xin-lin, QI Feng-hua, LU Yan, SHI Xiao-yu, HAN Xiao-li, XU Chun-sheng. Study on the Distribution of Glial Fibrillary Acidic Protein in Intestine of Yellow Feather Broiler[J]. , 2017, 44(5): 1315-1320.

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胶质纤维酸性蛋白在黄羽肉鸡肠道中的分布研究

Study on the Distribution of Glial Fibrillary Acidic Protein in Intestine of Yellow Feather Broiler

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