江苏省羊口疮病毒的分离鉴定及B2L基因的遗传进化分析

doi:10.16431/j.cnki.1671-7236.2017.04.002

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (4): 950-958.doi: 10.16431/j.cnki.1671-7236.2017.04.002

江苏省羊口疮病毒的分离鉴定及B2L基因的遗传进化分析

王秋霞^1,2, 朱相儒^1,2, 易成功^1,2, 秦涛^1,2, 陈素娟^1,2, 彭大新^1,2, 夏同海³

1. 扬州大学兽医学院, 扬州 225000;
2. 江苏省动物重要疫病与人兽共患防控协同创新中心, 扬州 225000;
3. 徐州睢宁魏集镇双惠养羊专业合作社, 徐州 221000

收稿日期:2016-09-30 出版日期:2017-04-20 发布日期:2017-05-03
通讯作者: 陈素娟 E-mail:chensj@yzu.edu.cn;pengdx@yzu.edu.cn
作者简介:王秋霞(1995-),女,江苏溧阳人,学士,研究方向:动物医学,E-mail: 1256886749@qq.com;朱相儒(1988-),女,河南焦作人,硕士,研究方向:动物传染病,E-mail: 1073752476@qq.com王秋霞和朱相儒对本文具有同等贡献,并列为第一作者
基金资助:
苏北科技专项资金（富民强县）项目（BN2014002）；扬州大学校科研创新项目（x2015718）；江苏高校优势学科建设工程项目；江苏高校品牌专业建设工程资助项目

Isolation and Identification of Orf Virus from Jiangsu Province and Phylogenetic Analysis of Their B2L Gene

WANG Qiu-xia^1,2, ZHU Xiang-ru^1,2, YI Cheng-gong^1,2, QIN Tao^1,2, CHEN Su-juan^1,2, PENG Da-xin^1,2, XIA Tong-hai³

1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225000, China;
2. Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose, Yangzhou 225000, China;
3. Goats'Professional Cooperatives of Shuang Hui in Weiji Town, Suining, Xuzhou 221000, China

Received:2016-09-30 Online:2017-04-20 Published:2017-05-03

摘要/Abstract

摘要：

为了解江苏省近年来羊口疮病毒（Orf virus，ORFV）的流行情况，更好地控制江苏地区的羊口疮病，2013~2015年采集江苏部分地区羊口疮疑似病料121份，进行病毒分离鉴定及其B2L基因的遗传进化分析。结果显示，样品中ORFV PCR检测阳性4份，用胎羊鼻甲骨细胞（OFTu）和MDBK细胞进行病毒分离，分离到4株病毒。这4株病毒分别命名为ORFV/Ovis/XZ/Jiangsu/2015/China、ORFV1/Ovis/DT/Jiangsu/2015/China、ORFV2/Ovis/DT/Jiangsu/2015/China和ORFV/Ovis/SL/Jiangsu/2015/China。扩增ORFV的B2L基因全长并绘制遗传进化树。B2L基因序列分析显示，4株分离株之间的核酸同源性为98.5%~100.0%。遗传进化树显示，ORFV/Ovis/XZ/Jiangsu/2015/China株、ORFV1/Ovis/DT/Jiangsu/2015/China株和ORFV2/Ovis/DT/Jiangsu/2015/China株与SC-JY、GX-YB、JS-FX株聚成一簇，核苷酸同源性为97.8%~100.0%。ORFV/Ovis/SL/Jiangsu/2015/China与LiaoNing、HuB、Gansu株亲缘关系接近，核苷酸同源性为98.8%~98.9%。4株分离株与中国疫苗株的核苷酸同源性为96.8%~98.1%。结果表明，江苏地区的ORFV来源可能不同，有必要开展更为深入的流行病学调查，为防控江苏地区的羊口疮奠定基础。

关键词: 羊口疮病毒; 胎羊鼻甲骨细胞; MDBK细胞; B2L基因; 遗传进化分析

Abstract:

The study was aimed to investigate prevalence of Orf virus (ORFV) in Jiangsu province in recent years and control Orf better. A total of 121 tissue samples were collected in some farms from 2013 to 2015 and subjected to PCR detection, viral isolation and phylogenetic analysis of B2L gene. Four samples were ORFV positive by PCR. The viruses were isolated by passaging in ovine fetal turbinate (OFTu) cells and MDBK cells, and were named as ORFV/Ovis/XZ/Jiangsu/2015/China, ORFV1/Ovis/DT/Jiangsu/2015/China, ORFV2/Ovis/DT/Jiangsu/2015/China and ORFV/Ovis/SL/Jiangsu/2015/China,respectively. The B2L gene was amplified and sequenced for the phylogenetic study. The nucleotide homology of these 4 strains was 98.5% to 100.0%. ORFV/Ovis/XZ/Jiangsu/2015/China, ORFV1/Ovis/DT/Jiangsu/2015/China and ORFV2/Ovis/DT/Jiangsu/2015/China, gathered into a cluster with SC-JY, GX-YB, JS-FX isolates and the nucleic acid homology of these strains was 97.8% to 100%. ORFV/Ovis/SL/Jiangsu/2015/China gathered into a cluster with LiaoNing, HuB and Gansu isolates, the nucleic acid homology was 98.8% to 98.9%. The nucleic acid homologies of 4 strains and ORFV strain China vaccine was 96.8% to 98.1%. The result showed that the ORFVs in Jiangsu province might be from different source. For controlling the spreading of this virus, it was necessary to carry out deep epidemiological survey in Jiangsu province.

Key words: Orf virus; OFTu cell; MDBK cell; B2L gene; phylogenetic analysis

中图分类号:

S852.65⁺9.1

王秋霞, 朱相儒, 易成功, 秦涛, 陈素娟, 彭大新, 夏同海. 江苏省羊口疮病毒的分离鉴定及B2L基因的遗传进化分析[J]. 《中国畜牧兽医》, 2017, 44(4): 950-958.

WANG Qiu-xia, ZHU Xiang-ru, YI Cheng-gong, QIN Tao, CHEN Su-juan, PENG Da-xin, XIA Tong-hai. Isolation and Identification of Orf Virus from Jiangsu Province and Phylogenetic Analysis of Their B2L Gene[J]. , 2017, 44(4): 950-958.

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江苏省羊口疮病毒的分离鉴定及B2L基因的遗传进化分析

Isolation and Identification of Orf Virus from Jiangsu Province and Phylogenetic Analysis of Their B2L Gene

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