合生素和抗菌肽对肉仔鸡大肠杆菌病的防制效果及免疫调节作用

doi:10.16431/j.cnki.1671-7236.2017.03.037

摘要/Abstract

摘要：

试验旨在研究添加自制合生素和新型抗菌肽对肉仔鸡生长性能的影响，以及攻毒后对其免疫功能的调节作用。选取健康的1日龄科宝Ross 308肉仔鸡100只，随机分为4组。自制合生素组（S组）饲喂基础日粮+0.3%合生素；抗菌肽组（AP组）饲喂基础日粮+0.5%抗菌肽；抗菌肽+合生素组（SAP组）饲喂基础日粮+0.3%合生素+0.5%抗菌肽；对照组（C组）饲喂基础日粮。试验期间测定各组肉鸡生长性能；在试验第14天，同时每组随机选取10只肉仔鸡进行大肠杆菌O78攻毒（对照组攻毒后设为攻菌模型组（M组）），观察记录24 h各组鸡的发病和死亡情况，并测定肉仔鸡的免疫功能。结果显示，与对照组相比，S组和AP组料重比和平均日采食量显著降低（P<0.05），SAP组的法氏囊指数在攻毒前显著升高（P<0.05），其他各组无显著差异（P>0.05）；攻毒后，S组及AP组的胸腺指数、脾脏指数及法氏囊指数均显著高于对照组（P<0.05），SAP组的脾脏指数、法氏囊指数均显著高于对照组（P<0.05），胸腺指数与对照组间无显著差异（P>0.05）。攻毒后S组中IgA、sIgA和IL-10与M组无显著差异（P>0.05），而IL-6极显著降低（P<0.01）；AP组中IgA和IL-6均极显著降低（P<0.01），sIgA和IL-10显著或极显著升高（P<0.05；P<0.01）；SAP组的变化趋势与AP组相同。综上所述，日粮中分别添加合生素和抗菌肽可以提高肉仔鸡的饲料转化率；攻菌后，合生素和抗菌肽以及两者联合使用均可以提高肉仔鸡免疫功能。

关键词: 合生素; 抗菌肽; 肉仔鸡; 大肠杆菌病; 免疫

Abstract:

This experiment was to study the effect of synbiotic and antibacterial peptide on growth performance,and the immune regulation of broiler chickens after the E.coli O78 attack. Totally 100 broilers of health 1-day-old Ross 308 were randomly divided into four treatment groups:Synbiotic group (group S) was fed with basal diet+0.3% synbiotic;Antibacterial peptides group (group AP) was fed with basal diet+0.5% antibacterial peptide; Antibacterial peptide+synbiotic group (group SAP) was fed with basal diet+0.3% synbiotic+0.5% antibacterial peptide;Control group (group C) was fed with basal diet,and the growth performance were determined. At the 14th day,10 chickens from each group were randomly selected for muscle injection with E.coli O78 (the attacked control group was named as model group (group M)),and after 24 h,the morbidity,mortality and immune function of chickens in each group were determined. The results showed that compared with control group,the F/G and ADF of groups S and AP were significantly decreased (P<0.05).Before E.coli O78 attack,the bursa index of group SAP was significantly higher than control group (P<0.05),and other groups had no significant difference (P>0.05);While after the E.coli O78 attack,the thymus,spleen and bursa indexes of groups S and AP were significantly higher than control group (P<0.05),the spleen and bursa indexes of group SAP were significantly higher than control group (P<0.05),and the thymus index showed no significant difference (P>0.05).Compared with the group M,the IgA,sIgA and IL-10 of group S showed no significant difference (P>0.05),while the IL-6 levels decreased extremely significantly (P<0.01);The IgA and IL-6 of group AP were extremely significantly decreased (P<0.01),meanwhile the sIgA and IL-10 were significantly or extremely significantly higher (P<0.05;P<0.01); The changed trend of SAP group was the same as the group AP.In conclusion,the synbiotic and antibacterial peptide could improve the feed conversion rate of chickens and they were used alone or combination all could improved chicken immune function after the bacteria attack.

Key words: synbiotics; antibacterial peptide; broiler chickens; colibacillosis; immune

中图分类号:

S816.73

朱琪, 郭明, 何岚, 杨春蕾, 李玉鹏, 李海花, 乔家运, 王建国, 王文杰. 合生素和抗菌肽对肉仔鸡大肠杆菌病的防制效果及免疫调节作用[J]. 《中国畜牧兽医》, 2017, 44(3): 888-895.

ZHU Qi, GUO Ming, HE Lan, YANG Chun-lei, LI Yu-peng, LI Hai-hua, QIAO Jia-yun, WANG Jian-guo, WANG Wen-jie. Effect of Synbiotic and Antibacterial Peptide on Colibacillosis and Immune Regulation in Broiler Chickens[J]. , 2017, 44(3): 888-895.

参考文献

[1] Ghunaim H,Abu-Madi M A,Kariyawasam S. Advances in vaccination against avian pathogenic Escherichia coli respiratory disease:Potentials and limitations[J].Vet Microbiol,2014,172(1-2):13-22.
[2] 朱焕星,王仁翠. 鸡大肠杆菌病及其综合防治措施[J].中国畜牧兽医文摘,2016,32(6):162.
[3] Mo S S,Slettemeås J S,Berg E S,et al. Plasmid and host strain characteristics of Escherichia coli resistant to extended-ppectrum cephalosporins in the Norwegian broiler production[J].PLoS One,2016,11(4):e154019.
[4] 张敬礼,王双山,张国政,等.中草药制剂对人工感染肉仔鸡大肠杆菌病防治效果的研究[J].中国畜牧兽医,2006,33(6):13-15.
[5] 王自然,阮明华.氟苯尼考琥珀酸钠对人工诱发鸡大肠杆菌病的疗效观察[J].中国畜牧兽医,2010,37(4):212-214.
[6] P?owiec A,S?awińska A,Siwek M Z,et al. Effect of in ovo administration of inulin and Lactococcus lactis on immune-related gene expression in broiler chickens[J].American Journal of Veterinary Research,2015,76(11):975-982.
[7] Onrust L,Ducatelle R,Van Driessche K,et al. Steering endogenous butyrate production in the intestinal tract of broilers as a tool to improve gut health[J].Frontiers in Veterinary Science,2015,2:75.
[8] Zhang L,Zhang L,Zhan X,et al. Effects of dietary supplementation of probiotic,clostridium butyricum, on growth performance,immune response,intestinal barrier function,and digestive enzyme activity in broiler chickens challenged with Escherichia coli K88[J].J Anim Sci Biotechnol,2016,7:3.
[9] Chaudhari A A,Kariyawasam S.An experimental infection model for Escherichia coli egg peritonitis in layer chickens[J].Avian Dis,2014,58(1):25-33.
[10] 王俊峰,陈雁南,温超,等.合生素对肉鸡生长性能、免疫器官指数、血清免疫指标及肠道菌群的影响[J].动物营养学报,2010,22(1):163-168.
[11] 胡新旭,孔令华,卞巧,等.益生菌和益生元在肉鸡上应用研究进展[J].饲料研究,2014,3:10-16.
[12] Saint-Cyr M J,Guyard-Nicodème M,Messaoudi S,et al. Recent advances in screening of anti-campylobacter activity in probiotics for use in poultry[J]. Frontiers in Microbiology,2016,7:553.
[13] 李可,罗建杰,孟昆,等. 益生菌对肉仔鸡生产性能、胴体性状、免疫功能和肉品质的影响[J].动物营养学报,2015,9(27):2903-2910.
[14] 李玲,杨桂芹.益生菌和异麦芽寡糖对肉仔鸡盲肠菌群及生产性能的影响[J].中国畜牧杂志,2008,44(1):32-34.
[15] 王静.抗菌肽对肉鸡生产性能和免疫功能的影响研究[D].泰安:山东农业大学,2007.
[16] Bao H,She R, Liu T,et al. Effects of pig antibacterial peptides on growth performance and intestine mucosal immune of broiler chickens[J].Poultry Science,2009,88(2):291-297.
[17] 都海明,陆兆新,王恬.抗菌脂肽对肉鸡抗氧化能力及血清生化指标的影响[J].畜牧与兽医,2010,42(6):8-13.
[18] 韩雪娇,马改彦,周玉岩,等.抗菌肽对肉鸡生产性能和免疫功能的影响[J].中国畜牧杂志,2014,50(7):70-74.
[19] 刘莉如,杨开伦,滑静,等.抗菌肽对蛋用仔公鸡生长性能、免疫指标及空肠组织相关细胞因子基因mRNA表达的影响[J].动物营养学报,2012,24(7):1345-1351.
[20] 屈军梅,陈平洁,李文平,等.莫家蝇抗菌肽提取及对鸡大肠杆菌病药效试验[J].中国畜牧兽医,2006,33(3):56-58.
[21] 马倩,赵衍铜,柏明娜,等.抗菌肽对芦花鸡生产性能和血清生化指标的影响[J].饲料研究,2012,3:27-29.
[22] 刘卫东,宋素芳,程璞.甘露寡糖和益生菌对肉仔鸡生产性能和肠道菌群的影响[J].家畜生态学报,2011,32(1):32-35.
[23] 孙全友,徐彬,王琳燚,等.抗菌肽和姜黄素对肉仔鸡生长性能、抗氧化功能及肠道微生物的影响[J].中国家禽,2015,37(20):32-36.
[24] 张连忠.酵母培养物对雏鸡生长性能、免疫器官发育和血清相关激素的影响[J].中国畜牧兽医,2011,38(4):33-37.
[25] 朴香淑,史彬林,李德发,等.保尔福对肉仔鸡生长、微生物菌群及免疫功能的影响[J].中国饲料,2004,11:24-26.
[26] 王秀青,张素芳,曹瑞兵,等.抗菌肽天蚕素B基因及其串联体在毕赤酵母中的表达[J].南京农业大学学报,2007,30(3):120-123.
[27] 张春杨,牛钟相,常维山,等. 益生菌剂对肉用仔鸡的营养、免疫促进作用[J].中国预防兽医学报,2002,24(1):51-54.
[28] 李亚杰,赵献军.益生菌、黄芪多糖微胶囊制剂对肉仔鸡生长性能、免疫器官指数及血液生化指标的影响[J].安徽农业科学,2007,35(1):100-103.
[29] 李丽.抗菌肽Abaecin制备及其对肉鸡生长及免疫影响的研究[D].呼和浩特:内蒙古农业大学,2010.
[30] Haghighi H R,Gong J,Gyles C L.Probiotics stimulate production of natural antibodies in chickens[J]. Clinical and Vaccine Immunology,2006,13(9):975-980.
[31] 申红,王俊刚,秦文彬,等. 饲喂蝇蛆抗菌肽对白痢鸡血液生化指标的影响[J].石河子大学学报(自然科学版),2011,29(5):566-569.
[32] Liu T,She R,Wang K,et al. Effects of rabbit sacculus rotundus antimicrobial peptides on the intestinal mucosal immunity in chickens[J].Poult Sci,2008,87(2):250-254.
[33] 周学东,施文元.口腔微生态学[M].北京:人民卫生出版社,2013.
[34] Yu J,Mookherjee N,Wee K,et al. Host defense peptide LL-37,in synergy with inflammatory mediator IL-1beta,augments immune responses by multiple pathways[J].J Immunol,2007,179(11):7684-7691.
[35] 彭翔,孙全友,李杰,等.抗菌肽和姜黄素对1~21日龄肉仔鸡生长性能和免疫功能的影响[J].动物营养学报,2014,26(2):474-481.
[36] 邵良平,周伦江,李国平,等.甘露寡糖和粪链球菌对鸡细胞免疫和肠道微生态的调节作用[J].中国兽医学报,2000,20(1):58-61.
[37] 孙全友,彭翔,李杰,等.姜黄素和抗菌肽对肉仔鸡生长性能和免疫机能的影响及其互作效应研究[J].中国畜牧杂志,2014,50(17):62-67.