CRISPR/Cas系统作用机制及其在寄生虫学研究中的应用

doi:10.16431/j.cnki.1671-7236.2017.01.029

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (1): 208-213.doi: 10.16431/j.cnki.1671-7236.2017.01.029

CRISPR/Cas系统作用机制及其在寄生虫学研究中的应用

宋海洋¹, 吴杰¹, 邹丰才^1,2, 刘洋³

1. 云南农业大学动物科学技术学院, 昆明 650201;
2. 云南省高校兽医公共卫生重点实验室, 昆明 650201;
3. 昆明医科大学第二附属医院妇科, 昆明 650101

收稿日期:2016-06-06 出版日期:2017-01-20 发布日期:2017-01-19
通讯作者: 刘洋 E-mail:13518735544@163.com
作者简介:宋海洋(1992-),男,河北承德人,硕士生,研究方向:兽医寄生虫病与寄生虫学,E-mail:472394896@qq.com
基金资助:
国家自然科学基金项目（31360607）；云南省应用基础研究计划重点项目（2013FA037）

Mechanism of CRISPR/Cas System and Its Application in Parasitology

SONG Hai-yang¹, WU Jie¹, ZOU Feng-cai^1,2, LIU Yang³

1. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
2. Key Laboratory of Veterinary Public Health of Higher Education of Yunnan Province, Kunming 650201, China;
3. Department of Gynecology, the Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China

Received:2016-06-06 Online:2017-01-20 Published:2017-01-19

摘要/Abstract

摘要：

CRISPR/Cas系统是一种存在于细菌和古细菌的免疫系统，该系统包括能靶向入侵宿主的病毒或外源DNA核酸片段的RNA和切割该片段的Cas9核酸酶。与其他基因编辑技术相比，该系统更易操作，效率更高，成本更低，被广泛应用于各种基因工程领域。作者综述了CRISPR/Cas系统的作用机制及其在寄生虫方面的应用。

关键词: CRISPR/Cas系统; Cas9核酸酶; 基因编辑

Abstract:

CRISPR/Cas system is an immune system present in bacteria and archaea that includes the RNA which has the ability to target viruses invading the host or exogenous DNA fragment of the nucleic acid and the Cas9 nuclease cutting the fragment. In view that the system is easier to operate, more efficient, lower cost, has been widely used in various fields of genetic engineering. Here, we demonstrated the mechanism of CRISPR/Cas systems and summarized the application in respect of the parasite.

Key words: CRISPR/Cas system; Cas9 nuclease; gene editing

中图分类号:

Q789

宋海洋, 吴杰, 邹丰才, 刘洋. CRISPR/Cas系统作用机制及其在寄生虫学研究中的应用[J]. 《中国畜牧兽医》, 2017, 44(1): 208-213.

SONG Hai-yang, WU Jie, ZOU Feng-cai, LIU Yang. Mechanism of CRISPR/Cas System and Its Application in Parasitology[J]. , 2017, 44(1): 208-213.

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CRISPR/Cas系统作用机制及其在寄生虫学研究中的应用

Mechanism of CRISPR/Cas System and Its Application in Parasitology

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