载脂蛋白CⅢ刺激猪主动脉血管内皮细胞差异表达基因的研究

doi:10.16431/j.cnki.1671-7236.2016.03.004

›› 2016, Vol. 43 ›› Issue (3): 585-591.doi: 10.16431/j.cnki.1671-7236.2016.03.004

载脂蛋白CⅢ刺激猪主动脉血管内皮细胞差异表达基因的研究

岳媛, 陈洪艳, 王嘉玮, 徐明强, 丁瑜, 姜昊, 高妍, 张嘉保, 闫守庆

吉林大学动物科学学院, 长春 130062

收稿日期:2015-11-10 出版日期:2016-03-20 发布日期:2016-03-30
通讯作者: 张嘉保, 闫守庆 E-mail:jluzhangjiabao@163.com;yansq@jlu.edu.cn
作者简介:岳媛(1991-),女,吉林长春人,硕士,研究方向:动物遗传育种,E-mail:4245856@qq.com
基金资助:
国家自然科学基金青年科学基金(31201761)

Analysis on Differential Expression Genes in Porcine Aortic Vascular Endothelial Cells Induced by Apolipoprotein C Ⅲ

YUE Yuan, CHEN Hong-yan, WANG Jia-wei, XU Ming-qiang, DING Yu, JIANG Hao, GAO Yan, ZHANG Jia-bao, YAN Shou-qing

College of Animal Sciences, Jilin University, Changchun 130062, China

Received:2015-11-10 Online:2016-03-20 Published:2016-03-30

摘要/Abstract

摘要： 本研究旨在探究载脂蛋白CⅢ (ApoCⅢ)刺激猪主动脉血管内皮细胞前后的差异基因表达谱,从而揭示ApoCⅢ的功能。利用酶解法成功分离猪主动脉血管内皮细胞并进行体外培养,采用高通量测序技术筛选出ApoCⅢ刺激前后的差异表达基因。结果表明,ApoCⅢ刺激猪主动脉血管内皮细胞前后共有647个差异表达基因,包括390个上调表达基因和257个下调表达基因。实时荧光定量PCR (qRT-PCR)检测表明,高通量测序数据结果正确可靠。GO及Pathway分析结果显示,差异表达基因的功能涉及免疫应答、细胞凋亡及死亡等。这表明ApoCⅢ可通过炎症反应、细胞黏附、细胞凋亡等分子通路影响猪主动脉血管内皮细胞的生理功能,为进一步解析ApoCⅢ引发动脉粥样硬化发生的分子机制提供了理论基础。

关键词: 载脂蛋白CⅢ; 猪主动脉血管内皮细胞; 高通量测序

Abstract: The aim of this study was to investigate the differential expression genes induced by ApoCⅢ,and study the function of ApoCⅢ.Porcine aortic vascular endothelial cells were successfully isolated using enzyme digestion,and then screened the differential expression genes induced by ApoCⅢ using the Solexa high-throughput sequencing technology.The results showed 647 differential expression genes,including 390 up-regulated genes and 257 down-regulated genes.The qRT-PCR results verified that the gene expression results from Solexa sequencing data were reliable.GO and Pathway analysis showed that the function of differential expression genes were related to immune response,cell apoptosis and death.These findings suggested that ApoCⅢ affected the physiological function of porcine aortic endothelial cells by the molecular pathways of inflammation,cell adhesion and apoptosis,which provided a theoretical basis for further understanding the molecular mechanisms of atherosclerosis caused by ApoCⅢ.

Key words: ApoCⅢ; porcine aortic vascular endothelial cell; high-throughput sequencing

中图分类号:

Q786

岳媛, 陈洪艳, 王嘉玮, 徐明强, 丁瑜, 姜昊, 高妍, 张嘉保, 闫守庆. 载脂蛋白CⅢ刺激猪主动脉血管内皮细胞差异表达基因的研究[J]. , 2016, 43(3): 585-591.

YUE Yuan, CHEN Hong-yan, WANG Jia-wei, XU Ming-qiang, DING Yu, JIANG Hao, GAO Yan, ZHANG Jia-bao, YAN Shou-qing. Analysis on Differential Expression Genes in Porcine Aortic Vascular Endothelial Cells Induced by Apolipoprotein C Ⅲ[J]. , 2016, 43(3): 585-591.

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载脂蛋白CⅢ刺激猪主动脉血管内皮细胞差异表达基因的研究

Analysis on Differential Expression Genes in Porcine Aortic Vascular Endothelial Cells Induced by Apolipoprotein C Ⅲ

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