›› 2013, Vol. 40 ›› Issue (7): 25-28.

• 生物技术 • 上一篇    下一篇

羊口疮病毒感染对巨噬细胞RAW264.7凋亡的影响

于永忠, 柳婧, 孙虎男, 冯丽, 崔玉东   

  1. 黑龙江八一农垦大学生命科学技术学院, 黑龙江大庆 163319
  • 修回日期:2013-02-27 出版日期:2013-07-20 发布日期:2013-07-24
  • 通讯作者: 崔玉东 E-mail:cuiyudong@yahoo.com
  • 作者简介:于永忠(1968-),男,山东人,博士,讲师,主要从事分子病毒学、分子免疫学及预防兽医学方面研究。
  • 基金资助:
    国家自然科学基金面上项目(31172353);黑龙江省教育厅科学技术研究项目(11551322)。

The Influence on Apoptosis of Macrophage RAW264.7 during ORFV Infection

YU Yong-zhong, LIU Jing, SUN Hu-nan, FENG Li, CUI Yu-dong   

  1. College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China
  • Revised:2013-02-27 Online:2013-07-20 Published:2013-07-24

摘要: 羊口疮(Orf)是由羊口疮病毒(ORFV) 引起的一种急性、接触性和高度嗜上皮性人畜共患的动物传染病,主要感染绵羊和山羊,有时威胁人类。本研究将ORFV(OV/HLJ/04株)纯化后作为试验材料,采用巨噬细胞系RAW264.7作为病毒细胞宿主,RAW264.7在LPS体外激活的情况下,接种ORFV,研究ORFV感染对巨噬细胞凋亡的影响。结果表明,用LPS处理的试验组巨噬细胞形态学改变较大,凋亡量也较大;而没用LPS处理过的试验组巨噬细胞变化则相对较少。Western blotting研究结果显示,细胞凋亡信号Caspase-3与甘油醛-3-磷酸脱氢酶(GAPDH)在24 h时对比,凋亡信号明显减弱,表明在24 h的巨噬细胞凋亡水平有所下降。

关键词: 羊口疮病毒; 巨噬细胞; 细胞凋亡; 差异性

Abstract: Orf was an acute skin zoonosis caused by Orf virus (ORFV), which could affect sheep, goats and humans. This research used purified ORFV (OV/HLJ/4 strains) as experimental material, the macrophage cell line RAW264.7 as host, RAW264.7 were classically activated by LPS before ORFV inoculation in vitro, the effect for macrophage apoptosis upon ORFV infection was investigated simultaneously. Comparison of morphological features of activation in the cells treated with LPS and non-LPS, these resulted in significant morphological variation each other. The apoptosis quantity of macrophage cells treated with the LPS was bigger than the non-LPS group which was less of relative changes. Western blotting showed that the apoptosis signal Caspase-3 weakened at 24 h comparing with glyceraldehyde 3-phosphate dehydrogenase (GAPDH), such a poptosis signal that was significantly weakened, related to that macrophage apoptosis level declined at that time.

Key words: Orf virus; macrophage; cell apoptosis; differentiation

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