›› 2013, Vol. 40 ›› Issue (7): 21-24.

• 生物技术 • 上一篇    下一篇

猪链球菌2型分解代谢控制蛋白A经NF-κB/MAPK通路对巨噬细胞RAW264.7影响的研究

郎需龙1,2, 冉会玲3, 王秀然3, 王凯1,2, 卜昭阳1,2, 张瑞1,2, 钱晶3, 陈思1,2, 万家余1,2, 王兴龙1,2   

  1. 1. 军事医学科学院军事兽医研究所, 吉林长春 130122;
    2. 吉林省人兽共患病预防与控制重点实验室, 吉林长春 130122;
    3. 吉林农业大学动物科学技术学院, 吉林长春 130118
  • 收稿日期:2012-11-27 出版日期:2013-07-20 发布日期:2013-07-24
  • 通讯作者: 王兴龙 E-mail:wangxl-2006@163.com
  • 作者简介:郎需龙(1978-),男,陕西人,博士,研究方向:兽医微生物学及免疫学。
  • 基金资助:
    国家自然科学基金项目(31101790/C1803)。

Research on the Effects of ccpA of Streptococcus suis Type 2 in RAW264.7 Macrophages though NF-κB/MAPK Pathway

LANG Xu-long1,2, RAN Hui-ling3, WANG Xiu-ran3, WANG Kai1,2, BU Zhao-yang1,2, ZHANG Rui1,2, QIAN Jing3, CHEN Si1,2, WAN Jia-yu1,2, WANG Xing-long1,2   

  1. 1. Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China;
    2. Jilin Province Key Laboratory for Zoonosis Prevention and Control, Changchun 130122, China;
    3. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China
  • Received:2012-11-27 Online:2013-07-20 Published:2013-07-24

摘要: 通过猪链球菌2型分解代谢控制蛋白A(catabolite control protein A,ccpA)原核表达蛋白对巨噬细胞RAW264.7进行刺激,结果发现,猪链球菌2型ccpA蛋白可以调节巨噬细胞NO产生,另外该蛋白质可通过对iNOS、NF-κB、p38 和 ERK 1/2 MAPK等的调节,从而影响巨噬细胞RAW264.7对细胞因子IL-6、TNF-α和IFN-γ的分泌。该研究为类似蛋白质对免疫细胞NF-κB/MAPK等通路调节影响及细胞因子产生条件探索建立了基础。

关键词: 猪链球菌2型; 分解代谢控制蛋白A; NF-κB/MAPK; 巨噬细胞RAW264.7

Abstract: In this study, we evaluated the stimulation of RAW264.7 macrophages with a purified expressed recombinant protein, catabolite control protein A (ccpA) of Streptococcus suis 2. The results showed that this protein could stimulate the secretion of NO and induce IL-6,TNF-α and IFN-γ in RAW264.7 macrophages if in a dose-dependent manner with specific pre-treatment of the cell cultures with iNOS, NF-κB, p38 and ERK 1/2 MAPK pathway inhibitors. This study might provide important proves for the development of regulatory factors on Streptococcus suis 2 infection.

Key words: Streptococcus suis type 2; catabolite control protein A (ccpA); NF-κB/MAPK; RAW264.7 macrophages

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