熟地黄多糖对环磷酰胺诱导的免疫抑制小鼠的免疫调节作用研究

doi:10.16431/j.cnki.1671-7236.2023.02.027

中国畜牧兽医 ›› 2023, Vol. 50 ›› Issue (2): 693-703.doi: 10.16431/j.cnki.1671-7236.2023.02.027

熟地黄多糖对环磷酰胺诱导的免疫抑制小鼠的免疫调节作用研究

徐倩倩¹, 杨万群², 王玉波³, 吴菲², 代娟娟⁴, 秦蜜蜜⁵, 庄金秋¹, 王艳¹, 沈志强¹, 李继昌⁶

1. 山东省滨州畜牧兽医研究院, 滨州 256600;
2. 青岛农业大学动物医学院, 青岛 266109;
3. 滨州医学院附属医院胸外科, 滨州 256600;
4. 滨州医学院附属医院临床实验中心, 滨州 256600;
5. 滨州医学院附属医院病理科, 滨州 256600;
6. 东北农业大学动物医学院, 哈尔滨 150030

收稿日期:2022-06-17 出版日期:2023-02-05 发布日期:2023-02-06
通讯作者: 徐倩倩 E-mail:qianqian19830125@163.com
作者简介:徐倩倩,E-mail:qianqian19830125@163.com
基金资助:
国家自然科学基金项目(31872513、31402243);山东省驴产业技术体系滨州综合试验站(SDAIT-27-12);滨州市生猪产业农业科技创新团队项目(BZNKC201816);山东省现代农业产业技术体系牛创新团队项目(SDAIT-09-12)

Study on Immunomodulatory Effect of Prepared Radix Rehmanniae Polysaccharide on Cyclophosphamide-induced Immunosuppression in Mice

XU Qianqian¹, YANG Wanqun², WANG Yubo³, WU Fei², DAI Juanjuan⁴, QIN Mimi⁵, ZHUANG Jinqiu¹, WANG Yan¹, SHEN Zhiqiang¹, LI Jichang⁶

1. Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, China;
2. Animal Medicine College, Qingdao Agricultural University, Qingdao 266109, China;
3. Department of Thoracic Surgery, Binzhou Medical College Hospital, Binzhou 256600, China;
4. Clinical Study Center, Binzhou Medical College Hospital, Binzhou 256600, China;
5. Department of Pathology, Binzhou Medical College Hospital, Binzhou 256600, China;
6. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China

Received:2022-06-17 Online:2023-02-05 Published:2023-02-06

摘要/Abstract

摘要： 【目的】研究熟地黄多糖对环磷酰胺诱导的免疫抑制小鼠的免疫调节作用,为熟地黄多糖用于临床治疗免疫低下疾病或开发相关保健食品提供科学依据。【方法】选用36只昆明系小鼠,随机分为6组:空白对照组、免疫抑制模型组、黄芪多糖组及熟地黄多糖低、中、高(分别灌胃熟地黄多糖50、100、200 mg/kg)剂量组(PRRPL、PRRPM、PRRPH),每组6只。除空白对照组外,其余各组小鼠均腹腔注射80 mg/kg环磷酰胺,建立免疫抑制模型。造模后各组小鼠分别灌胃相应药物,1 次/d,连续15 d,测定小鼠体重、腹腔巨噬细胞吞噬能力、免疫器官系数、脾淋巴细胞增殖及细胞周期、血清细胞因子含量,观察脾脏和胸腺组织形态学、肠道派氏节数目等免疫指标。【结果】与空白对照组相比,模型组脾脏指数极显著升高(P＜0.01),脾脏红髓、白髓界限不清,脾淋巴细胞数量减少;胸腺指数降低,皮质、髓质不清,结构破坏;脾淋巴细胞增殖能力、小鼠腹腔巨噬细胞吞噬能力及血清白细胞介素1β(IL-1β)、IL-6、γ-干扰素(INF-γ)水平均显著或极显著降低(P＜0.05;P＜0.01);脾淋巴细胞滞留在G0/G1期。与模型组相比,熟地黄多糖能极显著降低小鼠的脾脏指数(P＜0.01),缓解环磷酰胺造成的脾脏组织损坏,熟地黄多糖各剂量组对脂多糖(LPS)诱导的脾淋巴细胞增殖具有极显著的促进作用(P＜0.01),熟地黄多糖各剂量组能显著或极显著提高免疫抑制小鼠血清IL-1β、IL-6、肿瘤坏死因子α(TNF-α)、INF-γ水平(P＜0.05;P＜0.01),缓解淋巴细胞周期滞留,且高剂量组熟地黄多糖能显著提高小鼠腹腔巨噬细胞吞噬功能(P＜0.05)。造模前后及熟地黄多糖对肠道派氏结数量均无显著影响(P＞0.05)。【结论】熟地黄多糖能提高免疫抑制小鼠的免疫功能,可用于治疗免疫低下类疾病药物和保健品的开发。

关键词: 熟地黄多糖; 小鼠; 免疫抑制; 免疫调节

Abstract: 【Objective】 This study was aimed to investigate the immunomodulatory effects of prepared Radix Rehmanniae polysaccharide (PRRP) on cyclophosphamide (CTX)-induced immunesuppression in mice,and provide scientific evidence for the clinical use of PRRP on immune decrease diseases and development of dietary supplement.【Method】 Thirty six Kunming mice were randomly grouped into six groups:Blank control group,model group, Astragalus polysaccharide (APS) group,and three PRRP groups,which oral administer 50,100,200 mg/kg PRRP as PRRPL,PRRPM and PRRPH groups,respectively,six mice each group.Except normal control group,mice in other groups were given 80 mg/kg CTX via peritoneal injection to induce immunosuppression situation.After model was prepared,the mice were given drugs,once a day,for continually 15 days.The body weight,phagocytosis of peritoneal macrophage,immune organ index,tissue damage of thymus and spleen,splenic lymphocyte proliferation and cell cycle were measured,productions of cytokines in serum were determined,and Peyer’s patches (PPs) in intestinal were also counted.【Result】 Compared with blank control group,the index of spleen in model group was extremely significantly improved (P＜0.01),red and white pulp divided unclear,the number of splenic lymphocyte was decreased;The index of thymus was reduced,structure was destroyed with unclear cortex and medulla;The splenic lymphocyte proliferation,phagocytosis of peritoneal macrophage and interleukin-1 β (IL-1β),IL-6 and γ-interferon (INF-γ) in serum were significantly or extremely significantly decreased (P＜0.05; P＜0.01);The splenic lymphocytes were arrested in G0/G1 stage.Compared with model group,the spleen index in mice was extremely significantly decreased by PRRP (P＜0.01),the thymus and spleen structures were destroyed by CTX,the splenic lymphocyte proliferation was extremely significantly induced by advanced and lipopolysaccharide (LPS) (P＜0.01);The levels of IL-1β,IL-6,tumor necrosis factor α (TNF-α) and INF-γ in serum were significantly or extremely significantly improved (P＜0.05; P＜0.01);The splenic lymphocyte arrest was adjusted,and the phagocytosis of peritoneal macrophage in PRRPH group was significantly improved (P＜0.05).There were no significant changes of PRRP and before and after modeling on the number of PPs in intestinal (P＞0.05).【Conclusion】 PRRP could accelerate recovery of CTX-induced immunosuppression in mice,and could be used as drugs or dietary supplement for the treatment of immune decrease diseases.

Key words: PreparedRadix Rehmanniae Polysaccharide; mice; immunosuppression; immunomodulatory

中图分类号:

R285

徐倩倩, 杨万群, 王玉波, 吴菲, 代娟娟, 秦蜜蜜, 庄金秋, 王艳, 沈志强, 李继昌. 熟地黄多糖对环磷酰胺诱导的免疫抑制小鼠的免疫调节作用研究[J]. 中国畜牧兽医, 2023, 50(2): 693-703.

XU Qianqian, YANG Wanqun, WANG Yubo, WU Fei, DAI Juanjuan, QIN Mimi, ZHUANG Jinqiu, WANG Yan, SHEN Zhiqiang, LI Jichang. Study on Immunomodulatory Effect of Prepared Radix Rehmanniae Polysaccharide on Cyclophosphamide-induced Immunosuppression in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 693-703.

参考文献

[1] 张副兴,裘生梁,祝雨田,等.熟地黄多糖提取技术及药理作用研究进展[J].浙江中西医结合杂志,2013,23(2):153-154. ZHANG F X,QIU S L,ZHU Y T,et al.Research progress on extraction technology and pharmacological action of polysaccharide of Radix Rehmanniae Preparata[J]. Zhejiang Journal of Integrated Traditional Chinese and Western Medicine,2013,23(2):153-154.(in Chinese)
[2] 梁颖,徐绍娜,徐放,等.熟地黄多糖对环磷酰胺诱导小鼠的抗突变作用研究[J].中医药信息,2010,27(4):110-112. LIANG Y,XU S N,XU F,et al.The study of the antimutagenic effects of RPS on the mouse induced by cyclophosphamide[J]. Information on Traditional Chinese Medicine,2010,27(4):110-112.(in Chinese)
[3] CUI Y,YAN Z,HOU S,et al.Effect of Radix Rehmanniae Preparata on the expression of c-fos and NGF in hippocampi and learning and memory in rats with damaged thalamic arcuate nucleus[J]. Zhong Yao Cai,2004,27(8):589-592.
[4] 管斯琪,陈培丰,祝雨田,等.熟地黄多糖对阿霉素致小鼠骨髓抑制及免疫功能损伤的影响[J].浙江中医药大学学报,2014,38(3):312-315. GUAN S Q,CHEN P F,ZHU Y T,et al.Protective effect of polysaccharide extracted from Rehmanniae Preparata on adriamycin-induced myelosuppression and immunologic injury in mice[J]. Journal of Zhejiang Chinese Medical University,2014,38(3):312-315.(in Chinese)
[5] 苗明三,孙艳红,史晶晶,等.熟地黄粗多糖对血虚模型小鼠胸腺和脾脏组织形态的影响[J].中华中医药杂志,2007,22(5):318-320. MIAO M S,SUN Y H,SHI J J,et al.Effects of gross polysaccharide of Radix Rehmanniae Preparata on morphology changes of thymus and spleen in blood deficiency model mice[J]. China Journal of Traditional Chinese Medicine and Pharmacy,2007,22(5):318-320.(in Chinese)
[6] 杨兵,夏先林,施晓丽,等.熟地黄多糖对断奶仔猪抗氧化性能和免疫性能的影响[J].江苏农业学报,2012,28(4):787-791. YANG B,XIA X L,SHI X L,et al.Effects of Rehmannia polysaccharide on antioxidation and immunity in weaned piglets[J]. Jiangsu Journal of Agricultural Sciences,2012,28(4):787-791.(in Chinese)
[7] 杨颖,李志,黄俊明,等.免疫低下模型的建立及流式细胞术的检测研究[J].中国卫生检验杂志,2007,17(5):813-815. YANG Y,LI Z,HUANG J M,et al.A study of establishing immunosuppressive model in mice and application of flow cytometer in health food evaluation[J]. Chinese Journal of Health Laboratory Technology,2007,17(5):813-815.(in Chinese)
[8] 黄莉,闫实,孙晓璐,等.亚麻籽油对小鼠免疫功能的影响[J].中国油脂,2020,45(12):56-59. HUANG L,YAN S,SUN X L,et al.Effect of flaxseed oil on immune function of mice[J]. China Oils and Fats,2020,45(12):56-59.(in Chinese)
[9] YOO J H,LEE Y S,KU S,et al. Phellinus baumii enhances the immune response in cyclophosphamide-induced immunosuppressed mice[J]. Nutrition Research,2020,75:15-31.
[10] LANG S J,SCHMIECH M,HAFNER S,et al.Antitumor activity of an Artemisia annua herbal preparation and identification of active ingredients[J]. Phytomedicine,2019,62:152962.
[11] LIU Y,YANG A,QU Y,et al.Ameliorative effects of Antrodia cinnamomea polysaccharides against cyclophosphamide-induced immunosuppression related to Nrf2/HO-1 signaling in BALB/c mice[J]. International Journal of Biological Macromolecules, 2018,116:8-15.
[12] DALTRO S R T,SANTOS I P,BARROS P L,et al. In vitro and in vivo immunomodulatory activity of Physalis angulata concentrated ethanolic extract[J]. Planta Medica,2021,87(1-2):160-168.
[13] FENG H,FAN J,LIN L,et al.Immunomodulatory effects of phosphorylated Radix Cyathulae officinalis polysaccharides in immunosuppressed mice[J]. Molecules,2019,24(22):4150.
[14] LI L,JIANG Y J,YANG X Y,et al.Immunoregulatory effects on Caco-2 cells and mice of exopolysaccharides isolated from Lactobacillus acidophilus NCFM[J]. Food Function,2014,5(12):3261-3268.
[15] JAYARAMAN S,VARIYAR E J.Role of taraxerone isolated from Leucas lavandulifolia,as an immunomodulator[J]. Journal of Ethnopharmacology,2021,278:114307.
[16] 张伟杰.黄秋葵花多糖的结构、免疫调节活性及机制研究[D].镇江:江苏大学,2021. ZHANG W J.Study on the structure,immunomodulatory effects and mechanisms of a polysaccharide from Abelmoschus esculentus L.flowers[D].Zhenjiang:Jiangsu University,2021.(in Chinese)
[17] 高娟,李芳兰,周一珺,等.不同发育时期大鼠小肠粘膜及派氏结T淋巴细胞发育活化的研究[J].中国免疫学杂志,2009,25(8):678-683. GAO J,LI F L,ZHOU Y J,et al.Research on the development of intestinal mucosa and the development and activation of T lymphocyte in various periods[J]. Chinese Journal of Immunology,2009,25(8):678-683.(in Chinese)
[18] 牛佳卉,袁静,魏然,等.红枣多糖对小鼠肠道免疫屏障的保护作用及机制研究[J].食品工业科技,2021,42(4):295-300. NIU J H,YUAN J,WEI R,et al.Protective effect and mechanism study of jujube polysaccharides on intestinal immune barrier in mice[J]. Science and Technology of Food Industry,2021,42(4):295-300.(in Chinese)
[19] CASTAÑEDA-DELGADO J E,FRAUSTO-LUJAN I,GONZÁLEZ-CURIEL I,et al.Differences in cytokine production during aging and its relationship with antimicrobial peptides production[J]. Immunological Investigations,2017,46(1):48-58.
[20] HUANG D,CANCILLA M R,MORAHAN G.Complete primary structure,chromosomal localisation,and definition of polymorphisms of the gene encoding the human interleukin-12 p40 subunit[J]. Genes and Immunity, 2000,1:515.
[21] SOBOTA R M,MVLLER P J,KHOURI C,et al.SHPS-1/SIRP1 contributes to interleukin-6 signaling[J]. Cellular Signaling,2008,20:1385-1391.
[22] TANAKA T,NARAZAKI M,KISHIMOTO T.IL6 in inflammation,immunity,and disease[J]. Cold Spring Harbor Perspectives in Biology,2014,6:a016295.
[23] PARAMESWARAN N,PATIAL S.Tumor necrosis factor-α signaling in macrophages[J]. Critical Reviews in Eukaryotic Gene Expression,2011,20:87-103.
[24] SUGISAKI H,YAMANAKA K,KAKEDA M,et al.Increased interferon-γ,interleukin-12p40 and IL-8 production in Propionibacterium acnes-treated peripheral blood mononuclear cells from patient with acne vulgaris[J]. Journal of Dermatological Science,2009,55(1):47-52.
[25] YUE R,WEI X,ZHAO J,et al.Essential role of IFN-γ in regulating gut antimicrobial peptides and microbiota to protect against alcohol-induced bacterial translocation and hepatic inflammation in mice[J]. Frontiers in Physiology,2021,11:629141.

熟地黄多糖对环磷酰胺诱导的免疫抑制小鼠的免疫调节作用研究

Study on Immunomodulatory Effect of Prepared Radix Rehmanniae Polysaccharide on Cyclophosphamide-induced Immunosuppression in Mice

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