嗜酸乳杆菌对运输前后马肠道菌群的影响

doi:10.16431/j.cnki.1671-7236.2023.02.012

摘要/Abstract

摘要： 【目的】旨在研究运输应激对马肠道菌群结构的影响,并探讨嗜酸乳杆菌对马运输应激的预防作用。【方法】试验选取10匹24月龄的伊犁马,随机均分为试验组和对照组。所有马均进行环境适应1周后,试验组开始在饲喂基础饲粮外补饲嗜酸乳杆菌胶囊,对照组不补饲。饲喂15 d之后,试验组与对照组在第16天同时开展里程约400 km的运输试验,分别于运输前后采集对照组马颈静脉血液进行生理指标检测,并在喂菌前、喂菌15 d后和运输8 h后采集马粪便,试验组喂菌前样品设为A1组,喂菌15 d后样品设为A2组,运输8 h后样品设为A3组,对照组运输8 h后样品设为T3组。采用Illumina MiSeq高通量测序方法对马肠道菌群多样性进行分析。【结果】运输前后血液生理指标测定结果显示,对照组中间细胞比率和血红蛋白在运输8 h后呈现显著性下降(P＜0.05),血红蛋白浓度在运输8 h后极显著降低(P＜0.01)。补饲嗜酸乳杆菌和运输均能够引起马肠道菌群组成出现差异,补饲嗜酸乳杆菌增加了理研菌科RC9(Rikenellaceae_RC9_gut_group)、毛螺菌科AC2044属(Lachnospiraceae_AC2044_group)等有益菌的菌群丰度,而运输则增加了有害菌拟杆菌门(Bacteroidetes)和变形菌门(Proteobacteria)的数量。【结论】运输能够引起马产生应激反应,饲粮中添加嗜酸乳杆菌能够恢复运输应激带来的马肠道菌群紊乱,并增加了少量的有益菌,从而缓解运输造成的菌群紊乱现象,对机体产生有利影响。

关键词: 嗜酸乳杆菌; 肠道菌群; 马; 运输

Abstract: 【Objective】 The aim of this study was to investigate the effect of transport stress on the intestinal flora of horses,and to explore the preventive effect of Lactobacillus acidophilus on transport stress in horses.【Method】 For the experiment,ten 24-month-old Ili horses were chosen and randomly divided into test group and control group.After all horses had been acclimatized for one week,the test group was formally fed Lactobacillus acidophilus capsules in their diets,while the control group was fed the diets without any adjustment.After 15 d of feeding,the test group and the control group were transported at the same time on day 16 over a distance of about 400 km.The jugular blood of the control group was collected before and after the transportation for physiological indicators detection,and the horse feces were collected before feeding,15 days after feeding and 8 hours after transportation.The sample before feeding the bacteria in the test group was set as group A1,the sample after feeding the bacteria for 15 d was set as group A2,the sample after transportation for 8 h was set as group A3,and the sample after transportation for 8 h in the control group was set as group T3.Analyzing the variety of equine intestinal flora involved high-throughput sequencing on the Illumina MiSeq platform.【Result】 Blood physiological indices before and after transport in the control group showed that the intermediate cell ratio and hemoglobin were significant decreased (P＜0.05),and the hemoglobin concentration was extremely significantly decreased (P＜0.01) after 8 h of transport.Supplemental feeding of Lactobacillus acidophilus and transportation could cause differences in the composition of intestinal flora in horses.Supplemental feeding of Lactobacillus acidophilus increased the population of helpful bacteria,including Rikenellaceae_RC9_gut_group and Lachnospiraceae_AC2044_group,while transport increased the quantity of harmful bacteria,including Bacteroidetes and Proteobacteria.【Conclusion】 Transportation could cause stress reaction of horses.Adding Lactobacillus acidophilus to the diet could restore the intestinal flora disorder of horses caused by transportation stress,and increase a small amount of beneficial bacteria,so as to alleviate the flora disorder caused by transportation and had a beneficial impact on the body.

Key words: Lactobacillus acidophilus; gut microbiota; horse; transportation

中图分类号:

S821.7

银方方, 屈瑞, 叶梦珺, 候萌, 马学军, 彭啟敏, 陈伟丽, 卢亚宾, 买占海, 王金泉. 嗜酸乳杆菌对运输前后马肠道菌群的影响[J]. 中国畜牧兽医, 2023, 50(2): 543-555.

YIN Fangfang, QU Rui, YE Mengjun, HOU Meng, MA Xuejun, PENG Qimin, CHEN Weili, LU Yabin, MAI Zhanhai, WANG Jinquan. Effect of Lactobacillus acidophilus on the Intestinal Flora of Horses Before and After Transport[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 543-555.

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