2017-2019年山东省部分地区猪瘟流行病学调查

doi:10.16431/j.cnki.1671-7236.2020.06.027

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (6): 1870-1881.doi: 10.16431/j.cnki.1671-7236.2020.06.027

2017-2019年山东省部分地区猪瘟流行病学调查

王佳芸^1,2, 王鑫^2,3, 王炳煜^1,2, 曾昊², 沈文慧^2,4, 于江², 陈智², 张玉玉², 孙文博², 郭立辉², 任素芳², 李玉保¹, 吴家强^1,2,3,4

1. 聊城大学农学院, 聊城 252059;
2. 山东省畜禽疫病防治与繁育重点实验室, 济南 250100;
3. 临沂大学农林学院, 临沂 276000;
4. 山东农业大学动物科技学院, 泰安 271000

收稿日期:2019-11-14 出版日期:2020-06-20 发布日期:2020-06-20
通讯作者: 李玉保, 吴家强 E-mail:liyubao@lcu.edu.cn;wujiaqiang2000@sina.com
作者简介:王佳芸(1994-),女,山东烟台人,硕士生,研究方向:动物病毒学与免疫学,E-mail:769080627@qq.com;王鑫(1985-),女,山东济南人,教授,研究方向:动物病毒学与免疫学,E-mail:coala07@163.com
基金资助:
国家重点研发计划项目（2017YFD0500602）；山东省现代农业产业技术体系（SDAIT-08-06）；山东省农业重大应用技术创新项目；山东省农业科学院农业科技创新工程（CXGC2016B14、CXGC2018E11）；高层次人才工程经费资助（ts201511069、W03020496）

Epidemiological Investigation of CSFV in Shandong Province from 2017 to 2019

WANG Jiayun^1,2, WANG Xin^2,3, WANG Bingyu^1,2, ZENG Hao², SHEN Wenhui^2,4, YU Jiang², CHEN Zhi², ZHANG Yuyu², SUN Wenbo², GUO Lihui², REN Sufang², LI Yubao¹, WU Jiaqiang^1,2,3,4

1. College of Agriculture, Liaocheng University, Liaocheng 252059, China;
2. Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Jinan 250100, China;
3. College of Agriculture and Forestry Science, Linyi University, Linyi 276000, China;
4. College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, China

Received:2019-11-14 Online:2020-06-20 Published:2020-06-20

摘要/Abstract

摘要： 为掌握近年来山东地区猪瘟病毒（CSFV）的流行及其遗传变异情况，本研究对2017年1月-2019年12月从山东省济南市、泰安市、聊城市、青岛市等10个地区采集的1 687份疑似猪瘟感染猪病料运用反转录PCR方法进行了病毒核酸检测，将CSFV检测为阳性的样品进行E2基因和CSFV全基因扩增和测序，利用生物信息学软件DNAStar和Mega 7.0对CSFV的E2蛋白以及全基因序列进行遗传进化分析。另外，将这些地区采集的4 866份血清样本进行了抗体ELISA检测。结果显示，1 687份病料中病原检测有188份阳性，总阳性率为11.1%，其中2017-2019年病原检测阳性率分别为8.5%、13.1%、15.1%，抗原阳性率逐年上升且呈不稳定性，提示规模化猪场猪瘟兔化弱毒疫苗的免疫存在一定的风险和压力；采集的4 866份血清样本中4 146份呈现抗体阳性（85.2%），统计得到2017-2019年抗体阳性率分别为84.3%、83.0%、92.3%，抗体阳性率呈现上升趋势，说明山东省规模化养殖场猪群猪瘟疫苗免疫保护的整体水平逐年提高；从阳性病料中扩增获得了5株CSFV全基因组序列和5株病毒E2基因序列，经过序列比对发现，5株临床分离毒株与2.1d亚型的CSFV全基因组序列同源性在97.5%~99.0%之间，表明5株分离毒株与2.1d亚型的CSFV亲缘性较近，分离毒株氨基酸位点的突变集中在102位（L→M）以及159位（K→R）氨基酸处。本研究分析了2017年1月-2019年12月山东部分地区猪群中CSFV毒株的流行与变异情况，为指导山东省CSFV预防与控制提供了有力的参考。

关键词: 猪瘟病毒(CSFV); 分子流行病学; 全基因组; E2基因; 2.1d亚型

Abstract: To investigate the molecular epidemiology and genetic diversity of CSFV in Shandong province,1 687 tissue samples collected from January 2017 to December 2019 from suspected pigs in 10 regions (including Jinan,Taian,Liaocheng,Qingdao cities and so on) of Shandong province were detected for CSFV,the CSFV-positive samples were amplified and sequenced for E2 and CSFV gene,and the bioinformatics software DNAStar and Mega 7.0 were used to analyze the genetic evolution of CSFV E2 protein and the complete gene compared with reference strain.In addition,4 866 serum samples collected from the same regions as tissue samples were tested for E2-ELISA antibodies.The results showed that 188 out of 1 687 cases were positive for CSFV,with a total positive rate of 11.1%,and the annually positive rates from 2017 to 2019 were 8.5%,13.1% and 15.1%,respectively,it could be clearly seen that the antigen-positive rate had been increasing year by year,which might indicate that there was a certain risk and pressure in the immunization of swine fever rabbit attenuated vaccine in large-scale swine farms.While 4 146 of 4 866 blood samples (85.2%) were positive for E2 antibody,and the annually positive rates of E2 antibodies from 2017 to 2019 were 84.3%,83.0% and 92.3%,respectively.The positive rate of antibody detection had increased year by year,indicating that the overall level of swine fever vaccine protection in large-scale farms in Shandong province had increased year by year.In addition,5 strains of CSFV genome and 5 strains of virus E2 gene sequences were obtained from the positive disease material.The sequence homology between the 5 clinical isolates and the 2.1d subtype CSFV genome were 97.5% to 99.5%,indicating that the 5 isolates were closely related to the CSFV of the 2.1d subgenotype,mutations in the amino acid sites of the isolated strains were at positions 102 (L→M) and 159 (K→R).This study analyzed the prevalence and variation of CSFV in pigs in parts of Shandong from January 2017 to December 2019,and the results could provide a reference for the prevention and control in Shandong.

Key words: classical swine fever virus(CSFV); molecular epidemiology; complete genome; E2 gene; 2.1d subgenotype

中图分类号:

S852.65⁺1

王佳芸, 王鑫, 王炳煜, 曾昊, 沈文慧, 于江, 陈智, 张玉玉, 孙文博, 郭立辉, 任素芳, 李玉保, 吴家强. 2017-2019年山东省部分地区猪瘟流行病学调查[J]. 中国畜牧兽医, 2020, 47(6): 1870-1881.

WANG Jiayun, WANG Xin, WANG Bingyu, ZENG Hao, SHEN Wenhui, YU Jiang, CHEN Zhi, ZHANG Yuyu, SUN Wenbo, GUO Lihui, REN Sufang, LI Yubao, WU Jiaqiang. Epidemiological Investigation of CSFV in Shandong Province from 2017 to 2019[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1870-1881.

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2017-2019年山东省部分地区猪瘟流行病学调查

Epidemiological Investigation of CSFV in Shandong Province from 2017 to 2019

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