杜洛克猪HLCS基因多态位点与生长发育性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2020.06.019

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (6): 1800-1808.doi: 10.16431/j.cnki.1671-7236.2020.06.019

杜洛克猪HLCS基因多态位点与生长发育性状的相关性分析

蒲蕾^1,2, 代宇星¹, 张跃博², 张龙超², 郭亮¹, 王立贤²

1. 天津农学院动物科学与动物医学学院, 天津市农业动物繁育与健康养殖重点实验室, 天津 300384;
2. 中国农业科学院北京畜牧兽医研究所, 北京 100193

修回日期:2019-12-05 出版日期:2020-06-20 发布日期:2020-06-20
通讯作者: 郭亮, 王立贤 E-mail:liangguo177@vip.sina.com;iaswlx@263.net
作者简介:蒲蕾(1987-),女,陕西西安人,博士,研究方向:猪遗传育种,E-mail:pulei87@126.com
基金资助:
天津市教委科研计划项目（2018KJ188）；天津生猪产业技术体系创新团队（ITTPRS2017006）；中国农业科学院科技创新工程（ASTIP-IAS02）；国家生猪产业技术体系（CARS36）；北京市自然科学基金青年项目（6174047）；天津市种业专项（15ZXZYNC00100）；天津生猪畜产品加工产业发展研究（ZFZX2012-52）

Association Analysis on HLCS Gene Polymorphic Site with Growth and Development Traits in Duroc Pigs

PU Lei^1,2, DAI Yuxing¹, ZHANG Yuebo², ZHANG Longchao², GUO Liang¹, WANG Lixian²

1. Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Hubandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China;
2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Revised:2019-12-05 Online:2020-06-20 Published:2020-06-20

摘要/Abstract

摘要： 本研究旨在明确羧化全酶合成酶（holocarboxylase synthetase，HLCS）基因突变与杜洛克猪生长发育性状的相关性。利用Illumina SNP60芯片测序结果研究HLCS基因的突变位点，分析突变位点的多态性信息、突变位点与生长发育性状的关联性及突变位点的单倍型。结果显示，杜洛克猪HLCS基因具有4个SNPs位点：Ssc13：200455646 T>C、Ssc13：200458655 G>C、Ssc13：200504530 G>T、Ssc13：200551864 T>G，均符合Hardy-Weinberg平衡状态（P>0.05），呈现中度多态性。关联分析结果发现，Ssc13：200455646 T>C位点TT基因型100 kg背膘、剩余采食量均显著高于TC和CC基因型（P<0.05）；Ssc13：200458655 G>C位点GG基因型个体100 kg背膘、剩余采食量均显著高于CG和CC基因型（P<0.05）；Ssc13：200504530 G>T位点GT基因型平均日采食量、平均日增重均显著高于TT基因型（P<0.05），GT基因型个体100 kg日龄显著小于TT基因型（P<0.05）；Ssc13：200551864 T>G位点TG基因型平均日采食量、平均日增重均显著高于GG基因型（P<0.05），TG基因型个体100 kg日龄显著小于GG基因型（P<0.05）；其余性状各位点不同基因型间无显著差异（P>0.05）。Ssc13：200455646 T>C和Ssc13：200458655 G>C位点呈现高度连锁，Ssc13：200504530 G>T与Ssc13：200551864 T>G位点呈现高度连锁。结果表明，HLCS基因4个SNPs位点的多态性与杜洛克猪的剩余采食量、平均日采食量、平均日增重、100 kg日龄和100 kg背膘均具有显著相关性，可为猪的生长发育遗传改良奠定基础。

关键词: 杜洛克猪; HLCS基因; SNP; 生长发育性状

Abstract: The objective of this study was to clarify the correlation between SNP of holocarboxylase synthetase (HLCS) gene and growth and development traits in Duroc pigs.SNP of HLCS gene was obtained using the sequencing results of Illumina SNP60 chip,the polymorphism information of SNP,the correlation between SNP of HLCS gene and growth and development traits,and the haplotype of SNP were analyzed in this study.The results indicated that 4 SNPs (Ssc13:200455646 T>C,Ssc13:200458655 G>C,Ssc13:200504530 G>T and Ssc13:200551864 T>G) of HLCS gene were detected in Duroc pigs,which were in Hardy-Weinberg equilibrium (P>0.05),showing moderate polymorphism.The correlation analysis results showed that the 100 kg back fat (100 kg BF) and remaining food intake (RFI) of TT genotype in Ssc13:200455646 T>C were significantly higher than that of TC and CC genotypes (P<0.05).The 100 kg BF and RFI of GG genotype in Ssc13:200458655 G>C were significantly higher than that of CG and CC genotypes (P<0.05).The average daily feed intake (ADFI) and average daily gain (ADG) of GT genotype in Ssc13:200504530 G>T were significantly higher than that of TT genotype (P<0.05),the 100 kg age of GT genotype in Ssc13:200504530 G>T were significantly lower than that of TT genotype (P<0.05).The ADFI and ADG of TG genotype in Ssc13:200551864 T>G were significantly higher than that of GG genotype (P<0.05),the 100 kg age of TG genotype in Ssc13:200551864 T>G were significantly lower than that of GG genotype (P<0.05).There was no significant difference of other traits in different genotypes of SNPs (P>0.05).It showed high linkage of Ssc13:200455646 T>C and Ssc13:200458655 G>C,Ssc13:200504530 G>T and Ssc13:200551864 T>G,respectively.The results indicated that there were significant correlation between 4 SNPs of HLCS gene and RFI,ADFI,ADG,100 kg age and 100 kg BF in Duroc pigs,it could lay a foundation for the genetic improvement of growth and development in pigs.

Key words: Duroc pigs; HLCS gene; SNP; growth and development traits

中图分类号:

S813.3

蒲蕾, 代宇星, 张跃博, 张龙超, 郭亮, 王立贤. 杜洛克猪HLCS基因多态位点与生长发育性状的相关性分析[J]. 中国畜牧兽医, 2020, 47(6): 1800-1808.

PU Lei, DAI Yuxing, ZHANG Yuebo, ZHANG Longchao, GUO Liang, WANG Lixian. Association Analysis on HLCS Gene Polymorphic Site with Growth and Development Traits in Duroc Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1800-1808.

参考文献

[1] VAN ISEGHEM V,SPRENGERS M,DE ZAEYTIJD J,et al.Biotinidase deficiency:A treatable cause of opticospinal syndrome in young adults[J].Multiple Sclerosis and Related Disorders,2019,32:64-65.
[2] 朱勇文,侯水生,杨琳.生物素对营养代谢调控的研究进展[J].饲料工业,2011,32(9):12-14. ZHU Y W,HOU S S,YANG L.Research progress on the regulation of nutrient metabolism by biotin[J].Feed Industry,2011,32(9):12-14.(in Chinese)
[3] WOLF B.Clinical issues and frequent questions about biotinidasedeficiency[J].Molecular Genetics and Metabolism,2010,100(1):6-13.
[4] GULIYEVA U,OKUR I,DULAC O,et al.Epilepsy inbiotinidase deficiency is distinct from early myoclonic encephalopathy[J].Neuropediatrics,2018,49(6):417-418.
[5] OH W,ABU-ELHEIGA L,KORDARI P,et al.Glucose and fat metabolism in adipose tissue of acetyl-CoA carboxylase 2 knockout mice[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(5):1384-1389.
[6] JITRAPAKDEE S,ST MAURICE M,RAYMENT I,et al.Structure,mechanism and regulation of pyruvate carboxyase[J].Biochemical Journal,2008,413(3):369-387.
[7] DO D N,OSTERSEN T,STRATHE A B,et al.Genome-wide association and systems genetic analyses of residual feed intake,daily feed consumption,backfat and weight gain in pigs[J].BMC Genetics,2014,15:27.
[8] 张跃博,蒲蕾,张金山,等.杜洛克猪HLCS基因组织表达分析及其编码区多态性与剩余采食量的关联[J].畜牧兽医学报,2018,49(6):1108-1115. ZHANG Y B,PU L,ZHANG J S,et al.Tissue expression profiles of HLCS gene and its association with residual feed intake in Duroc[J].Acta Veterinaria et Zootechnica Sinica,2018,49(6):1108-1115.(in Chinese)
[9] GROSS A S,ZIMMERMANN A,PENDL T,et al.Acetyl-CoA carboxylase 1-dependent lipogenesis promotes autophagy downstream of AMPK[J].Journal of Biological Chemistry,2019,294(32):12020-12039.
[10] 苟兴能,吴照民,王熙,等.生物素对猪生长性能和肉品质的影响[J].饲料工业,2012,33(12):4-6. GOU X N,WU Z M,WANG X,et al.Effects of biotin on growth performance and meat quality of pigs[J].Feed Industry,2012,33(12):4-6.(in Chinese)
[11] ONTERU S K,GORBACH D M,YOUNG J M,et al.Whole genome association studies of residual feed intake and related traits in the pig[J].PLoS One,2013,8(6):e61756.
[12] 蒲蕾,岳静伟,张金山,等.杜洛克猪MAP3K5基因多态位点与生长、饲料利用性状的关联分析[J].中国畜牧兽医,2016,11(43):2982-2988. PU L,YUE J W,ZHANG J S,et al.Association analysis on MAP3K5 gene polymorphism site with growth and feed efficiency ralated traits in Duroc pigs[J].China Animal Husbandry & Veterinary Medicine,2016,11(43):2982-2988.(in Chinese)
[13] 农业部办公厅.全国生猪遗传改良计划(2009-2020)[J].中国牧业通讯,2010,23:22-25. GENERAL OFFICE OF THE MINISTRY OF AGRICULTURE.National pig genetic improvement plan (2009-2020)[J].China Animal Husbandry Bulletin,2010,23:22-25.(in Chinese)
[14] 张金山.杜洛克猪饲料利用效率参数估计及候选基因HLCS关联分析[D].北京:中国农业科学院,2017. ZHANG J S.Determination of feed efficiency in Duroc pig and research of the important candidate HLCS[D].Beijing:Chinese Academy of Agricultural Sciences,2017.(in Chinese)
[15] ABO ALROB O,LOPASCHUK G D.Role of CoA and acetyl-CoA in regulating cardiac fatty acid and glucose oxidation[J].Biochemical Society Transactions,2014,42(4):1043-1051.
[16] WONGKITTICHOTE P,AH MEW N,CHAPMAN K A.Propionyl-CoA carboxylase:A review[J].Molecular Genetics and Metabolism,2017,122(4):145-152.
[17] GALLARDO M E,DESVIAT L R,RODRIGUEZ J M,et al.The molecular basis of 3-methylcrotonylglycinuria,a disorder of leucine catabolism[J].American Journal of Human Genetics,2001,68(2):334-346.
[18] CANTLEY J,DAVENPORT A,VETTERLI L,et al.Disruption of beta cell acetyl-CoA carboxylase-1 in mice impairs insulin secretion and beta cell mass[J].Diabetologia,2019,62(1):99-111.
[19] LEON-DEL-RIO A.Biotin in metabolism,gene expression,and human disease[J].Journal of Inherited Metabolic Disease,2019,42(4):647-654.
[20] 邓奇风,高凤仙.生物素的营养代谢调控及生理功能研究进展[J].饲料与畜牧,2016,9:33-35. DENG Q F,GAO F X.Research progress on nutrient metabolism regulation and physiological function of biotin[J].Feed and Animal Husbandry,2016,9:33-35.(in Chinese)
[21] KORNEGAY E T.Biotin in swine nutrition[J].Annals of the New York Academy of Sciences,1985,447:112-121.
[22] GALIC S,LOH K,MURRAY-SEGAL L,et al.AMPK signaling to acetyl-CoA carboxylase is required for fasting- and cold-induced appetite but not thermogenesis[J].Elife,2018,7:e32656.
[23] SONE H,KAMIYAMA S,HIGUCHI M,et al.Biotin augments acetyl CoA carboxylase 2 gene expression in the hypothalamus,leading to the suppression of food intake in mice[J].Biochemical and Biophysical Research Communications,2016,476(3):134-139.

杜洛克猪HLCS基因多态位点与生长发育性状的相关性分析

Association Analysis on HLCS Gene Polymorphic Site with Growth and Development Traits in Duroc Pigs

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张蕊, 穆春宇, 汤青萍, 卜柱, 杨明军, 夏爱萍, 杨晓明. 鸽GnIH基因多态性及其与产蛋性状的关联分析[J]. 中国畜牧兽医, 2023, 50(4): 1412-1421.
[2]	龙安炬, 杨酸, 艾锦新, 翁吉梅, 张依裕, 蔡惠芬, 罗卫星. 黔北麻羊MC4R基因多态性及其与生长性状的关联分析[J]. 中国畜牧兽医, 2023, 50(1): 198-209.
[3]	徐昊祺, 许静漪, 胡丽蓉, 张帆, 罗汉鹏, 张海亮, 师睿, 李想, 刘林, 刘巧香, 郭刚, 王雅春. 中国荷斯坦牛PIK3CB基因多态性及其与繁殖和产奶性状的关联分析[J]. 中国畜牧兽医, 2022, 49(9): 3438-3452.
[4]	冯小品, 李隐侠, 张晨俭, 张俊, 孟春花, 钱勇, 曹少先. 波尔山羊PLAG1基因克隆、多态性鉴定及其与出生体重、体尺性状的关联分析[J]. 中国畜牧兽医, 2022, 49(9): 3465-3474.
[5]	李望娇, 彭夏, 宋徽, 董文君, 李新云, 赵书红, 马云龙. 基于选择信号分析揭示猪终端父本群体间性状趋同的关键基因[J]. 中国畜牧兽医, 2022, 49(8): 2982-2991.
[6]	周雅, 张禾垟, 刘琳玲, 李浩东, 郑军军, 王桂武. 梅花鹿成纤维细胞因子受体2基因多态性及其与茸重性状的关联分析[J]. 中国畜牧兽医, 2022, 49(8): 3006-3014.
[7]	阮涌, 陈祥, 代林刚, 黄家锦, 安冬伟, 邹启顺. F3代波杂山羊VNN1基因多态性及其与产羔数的关联分析[J]. 中国畜牧兽医, 2022, 49(4): 1384-1392.
[8]	邓伊华, 王天娇, 王洪亮, 董依萌, 刘欣, 邢秀梅. 基于重测序技术的塔河马鹿特异性SNP位点筛选[J]. 中国畜牧兽医, 2022, 49(4): 1413-1421.
[9]	姜宏正, 杨德智, 马中华, 荀文娟, 施力光, 侯冠彧. 儋州鸡体尺性状全基因组关联分析[J]. 中国畜牧兽医, 2022, 49(2): 598-607.
[10]	周雅, 张禾垟, 刘琳玲, 李浩东, 郑军军, 王桂武. 梅花鹿MSRB3基因多态性及其与茸重性状的关联分析[J]. 中国畜牧兽医, 2022, 49(11): 4279-4287.
[11]	路玉洁, 莫家远, 朱思燃, 杨丽丽, 陈奎蓉, 吕冬玲, 李月月, 刘笑笑, 梁靓, 兰干球, 梁晶. 利用中芯一号SNP芯片检测隆林猪全基因组拷贝数变异[J]. 中国畜牧兽医, 2022, 49(1): 23-31.
[12]	赵玉强, 陈鑫, 李清春, 马继林, 董银河, 汪德明, 郭凌云, 黄涛. 大白猪ENO3基因多态性及其与生长性状的关联分析[J]. 中国畜牧兽医, 2022, 49(1): 188-196.
[13]	刘鹏程, 张继, 邱淦远, 龚俞, 李雪松, 李维, 张依裕, 刘若余. 关岭牛TBC1D7基因多态性及其与生长性状的关联分析[J]. 中国畜牧兽医, 2021, 48(8): 2853-2863.
[14]	张珈溯, 郭思玮, 张馨心, 张聪聪, 张帅, 尹宝珍, 张洛萌, 夏广军. 延边黄牛脂蛋白脂肪酶基因多态性及其与肉质性状的关联分析[J]. 中国畜牧兽医, 2021, 48(7): 2530-2538.
[15]	田佺, 王思寒, 刘磊, 赵祎, 柳海星, 金花子, 李钟淑, 张君正, 戢爽. 延边黄牛MYH3和MYH8基因多态性及其与生长性状的关联分析[J]. 中国畜牧兽医, 2021, 48(6): 2122-2128.