中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (2): 392-398.doi: 10.16431/j.cnki.1671-7236.2020.02.009

• 生物技术 • 上一篇    下一篇

基于转录组测序生物信息学分析的研究进展

宋尚桥1, 马围围1, 张超龙1, 曾素先2, 孙翠翠1, 李鑫1, 严瑾1, 黎宗强1   

  1. 1. 广西大学动物科学技术学院, 南宁 530004;
    2. 南宁市江南区沙井街道办事处水产畜牧兽医站, 南宁 530045
  • 收稿日期:2019-08-10 发布日期:2020-02-28
  • 通讯作者: 黎宗强 E-mail:zqingli@gxu.edu.cn
  • 作者简介:宋尚桥(1995-),男,安徽滁州人,硕士生,研究方向:动物繁殖生物学,E-mail:1157677030@qq.com;马围围(1994-),女,安徽亳州人,硕士生,研究方向:动物繁殖生物学,E-mail:2535593418@qq.com
  • 基金资助:
    广西壮族自治区科技计划项目—重点研发计划(桂科AB16380072)

A Advances in Bioinformatics Analysis Based on Transcriptome Sequencing

SONG Shangqiao1, MA Weiwei1, ZHANG Chaolong1, ZENG Suxian2, SUN Cuicui1, LI Xin1, YAN Jin1, LI Zongqiang1   

  1. 1. College of Animal Science and Technology, Guangxi University, Nanning 530004, China;
    2. Shajing Subdistrict Office, Jiangnan District, Nanning City Aquatic Animal Husbandry and Veterinary Station, Nanning 530045, China
  • Received:2019-08-10 Published:2020-02-28

摘要: 随着DNA双螺旋结构的发现,基因组研究开始进行,中心法则也成为了研究生命科学的核心。这对近年来分子生物学及检测技术的迅猛发展提供了良好的开端,随之而来的是基因测序研究成为了生物学上一个新的热点话题。虽然通过转录组测序可以获得海量基因数据,但是数据的深入探索还需要进一步研究,而这些深入的挖掘,需要生物信息学分析手段,从而建立了多组学,通过对其进行不断完善,使得现代生物科学形成了一个完整的系统。这使得生物基因学的研究可以成为一个逐层的分析,将通过整合分析出的信息数据来描述生命活动。因此,功能基因组的研究已经成为了动植物基因组研究的重要方向,特别是对转录组的研究尤为重要。作者通过对转录组学的时代背景、转录组研究的复杂性、DNA测序技术的发展史以及转录组学研究方法进行归纳,简述了转录组学的发展历程,并对转录组研究内容做了一个简单系统的介绍,为更加深入地了解转录组测序奠定了基础。

关键词: 分子生物学; 转录组测序; 基因数据; 生物信息学; DNA测序

Abstract: With the discovery of the double helix structure of DNA,a preliminary study of genome has been carried out,and the central principle has become the core of life science.It has provided a beneficial start for the rapid development of molecular biology and detection technology in recent years.With this,animal gene sequencing research has become a new hot topic in biology.Although a great deal of gene data can be obtained by transcriptome sequencing,the further exploration of data needs further research,and these in-depth mining needs bioinformatics analysis tools,thus establishing a multi-group science,through its continuous improvement,making modern biological science form a complete system.It enables the study of biogenetics to be a layer-by-layer analysis that integrates information and data to describe life activities.Therefore,functional genome research has became an important direction of animal and plant genome research,especially for transcriptome research.This review summarized the background of transcriptome,the complexity of transcriptome research,the development history of DNA sequencing technology and the methods of transcriptome research.It briefly described the development of transcriptome and gave a simple and systematic introduction to the content of transcriptome research,so as to better understand transcriptome sequencing.It laid a foundation for a deeper understanding of transcriptome sequencing.

Key words: molecular biology; transcriptome sequencing; genetic data; bioinformatics; DNA sequencing

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