新疆褐牛乳房炎抗性相关基因的表达及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2020.01.011

摘要/Abstract

摘要： 文章旨在分析新疆褐牛乳房炎抗性相关基因的分子生物学功能，为分子标记辅助育种工作在新疆褐牛乳房炎抗性性状中的研究奠定理论基础。试验采用DAVID 6.8软件对课题组前期研究及相关文献报道的乳房炎抗性相关基因（共20个基因）进行功能GO富集及KEGG信号通路分析。采用String 10.0数据库进行蛋白相互作用网络分析，结合生物信息学分析和相关文献的研究结果，挑选出JAK2和STAT5A基因，通过使用实时荧光定量PCR技术检测JAK2、STAT5A基因在不同乳房健康程度的新疆褐牛血液中的相对表达量。GO富集分析得到，20个基因主要涉及细胞膜、免疫应答和调节防御反应等生物过程；KEGG富集分析发现，这些基因主要参与JAK-STAT信号通路、趋化因子信号通路、ErbB信号通路等；PPI分析发现，20个基因中有8个基因之间存在直接或间接联系；实时荧光定量试验发现，JAK2、STAT5A基因在健康新疆褐牛血液的表达量极显著或显著高于患乳房炎新疆褐牛（P<0.01；P<0.05）。通过生物信息分析和实时荧光定量试验，进一步从分子水平探讨了不同乳房健康程度的新疆褐牛与JAK2、STAT5A基因表达之间的内在联系。

关键词: JAK2基因; STAT5A基因; 实时荧光定量PCR; 乳房炎抗性; 新疆褐牛

Abstract: The aim of this study was to analyze the molecular biological functions of mastitis resistance related genes in Xinjiang Brown cattle,and lay a theoretical foundation for further development of mastitis resistance traits in Xinjiang Brown cattle by molecular marker-assisted breeding.Functional GO enrichment analysis and KEGG signaling pathway analysis were performed by DAVID 6.8 software for the genes related to mastitis resistance reported (20 genes in total) in previous studies and related literature.Using String 10.0 database for protein interaction network analysis,combined with bioinformatics analysis results and related literature research results,JAK2 and STAT5A genes were selected.Real-time PCR was used to detect the expression of JAK2 and STAT5A genes in blood of Xinjiang Brown cattle with different breast health.GO enrichment analysis showed that 20 genes mainly involved biological processes such as cell membrane and immune response regulation defense reaction;KEGG analysis found that genes were mainly involved in JAK-STAT signaling pathway,chemokine signaling pathway,ErbB signaling pathway,etc;PPI analysis showed that there were direct or indirect links between 8 genes in the 20 genes.Fluorescence quantitative test showed that the expression of JAK2 and STAT5A genes in blood of healthy Xinjiang Brown cattle was extremely significantly or significantly higher than that of mastitis Xinjiang Brown cattle(P<0.01;P<0.05).The JAK2 and STAT5A genes were screened by bioinformatics analysis.Furthermore,the intrinsic relationship between Xinjiang Brown cattle with different breast health and JAK2 and STAT5A gene expression was discussed at the molecular level.

Key words: JAK2 gene; STAT5A gene; Real-time PCR; mastitis resistance; Xinjiang Brown cattle

中图分类号:

S813.3

姜徽, 刘攀, 李媛媛, 刘奎国, 黄锡霞, 刘江卫, 胥磊, 杜建文, 桑扎根, 杨雪梅, 赵番番, 王丹. 新疆褐牛乳房炎抗性相关基因的表达及生物信息学分析[J]. 中国畜牧兽医, 2020, 47(1): 90-97.

JIANG Hui, LIU Pan, LI Yuanyuan, LIU Kuiguo, HUANG Xixia, LIU Jiangwei, XU Lei, DU Jianwen, SANG Zhagen, YANG Xuemei, ZHAO Fanfan, WANG Dan. Expression and Bioinformatics Analysis of Mastitis Resistance Related Genes in Xinjiang Brown Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 90-97.

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