福清山羊和戴云山羊夏季发情期卵巢组织比较转录组分析

doi:10.16431/j.cnki.1671-7236.2019.11.017

摘要/Abstract

摘要： 试验旨在从分子遗传学角度探讨戴云山羊夏季维持较高繁殖力的遗传机制，从中发掘有用的遗传变异信息。利用转录组测序方法对福清山羊和戴云山羊夏季发情期卵巢组织进行研究，筛选品种间的差异表达基因（differentially expressed genes，DEGs），利用基因本体（gene ontology，GO）、蛋白相邻类的聚簇（cluster of orthologous groups of proteins，COG）和京都基因与基因组百科全书（kyoto encyclopedia of genes and genomes，KEGG）数据库对筛选的差异表基因进行功能注释，并进行差异表达基因聚类分析，通过实时荧光定量PCR验证测序结果。结果显示，福清山羊和戴云山羊发情期卵巢的DEGs共357个，其中上调基因221个，下调基因136个。357个DEGs中的287个基因能够被GO数据库注释，78个DEGs能够被COG数据库注释，219个DEGs能够被KEGG数据库注释。KEGG数据库注释结果显示，这些差异基因显著富集的信号通路为蛋白消化吸收（protein digestion and absorption）、吞噬体（phagosome）、肺结核（tuberculosis）、胞外基质受体互作（ECM-receptor interaction）、金黄色葡萄球菌感染（Staphylococcus aureus infection）、同种异体移植物排斥（allograft rejection）等通路。经实时荧光定量PCR验证，所选基因（转录本）表达变化模式与转录组测序结果一致，表明测序结果可靠。本试验利用高通量测序获得大量山羊卵巢转录组信息，有助于从分子水平对山羊卵巢进行深入研究。

关键词: 福清山羊; 戴云山羊; 卵巢; 转录组; 差异表达基因

Abstract: In order to reveal the molecular mechanism of superior fecundity of Daiyun goat in summer season and find out the useful genetic variation in ovary.Transcriptome sequencing of ovary tissue in Fuqing and Daiyun goats were performed by Illumina HiSeq.Differentially expressed genes (DEGs) were screened using gene ontology (GO),cluster of orthologous groups of proteins (COG) and kyoto encyclopedia of genes and genomes (KEGG) databases,which were verified by Real-time quantitative PCR.The results showed that there were 357 DEGs in Fuqing and Daiyun goats,221 DEGs were up-regulated and 136 DEGs were down-regulated.Cluster analysis results showed that 287,78 and 219 DEGs annotated into the GO,COG and KEGG databases,respectively.KEGG analysis results showed that 219 DEGs were mainly enriched in protein digestion and absorption,phagosome,tuberculosis,ECM-receptor interaction, Staphylococcus aureus infection and allograft rejection.Real-time quantitative PCR results showed that the expression patterns of the selected gene (transcript) were consistent with that of transcriptome sequencing,indicating that the sequencing results were reliable.In this study,a large amount of ovarian transcriptome information in goat was obtained by high-throughput sequencing,which was helpful to further study the ovary in goats at the molecular level.

Key words: Fuqing goats; Daiyun goats; ovary; transcriptome; differentially expressed genes(DEGs)

中图分类号:

S827

吴贤锋, 李文杨, 刘远, 张莹, 黄勤楼. 福清山羊和戴云山羊夏季发情期卵巢组织比较转录组分析[J]. 中国畜牧兽医, 2019, 46(11): 3277-3288.

WU Xianfeng, LI Wenyang, LIU Yuan, ZHANG Ying, HUANG Qinlou. Comparative Transcriptome Analysis of Ovary Tissues in Fuqing and Daiyun Goats During Summer Estrus[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3277-3288.

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