中国畜牧兽医 ›› 2019, Vol. 46 ›› Issue (10): 2813-2822.doi: 10.16431/j.cnki.1671-7236.2019.10.001

• 生物技术 • 上一篇    下一篇

基于RNA-seq数据鉴定苏姜猪肉质性状相关基因

徐盼1,2, 何鑫鑫1, 倪黎纲1,2, 唐子雪1, 杨持1, 朱淑斌1, 季鸿鸣1,2, 周春宝1,2   

  1. 1. 江苏农牧科技职业学院, 泰州 225300;
    2. 江苏苏姜种猪有限公司, 泰兴 225411
  • 收稿日期:2019-02-13 出版日期:2019-10-20 发布日期:2019-10-21
  • 通讯作者: 周春宝 E-mail:zhou_tz@163.com
  • 作者简介:徐盼(1988-),男,江苏南京人,博士,研究方向:动物遗传育种,E-mail:panxu_nj@hotmail.com
  • 基金资助:
    江苏省科协青年科技人才托举工程(201898);江苏省大学生创新创业训练计划项目(201812806005Y、201812806037H);江苏省高职院校青年教师企业实践培训项目(2018QYSJ038);江苏农牧科技职业学院自然科学基金(NSF200701、NSFPT201802)

Identification of Meat Quality Traits Related Genes in Sujiang Pig Based on RNA-seq Data

XU Pan1,2, HE Xinxin1, NI Ligang1,2, TANG Zixue1, YANG Chi1, ZHU Shubin1, JI Hongming1,2, ZHOU Chunbao1,2   

  1. 1. Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, China;
    2. Jiangsu Sujiang Breeding Pig Co., Ltd., Taixing 225411, China
  • Received:2019-02-13 Online:2019-10-20 Published:2019-10-21

摘要: 试验通过对苏姜猪背最长肌和腿肌进行转录组分析,旨在挖掘影响苏姜猪肌肉肉质性状的候选基因。选取90和180日龄苏姜猪各3头,利用RNA-seq技术对90日龄苏姜猪背最长肌、180日龄苏姜猪背最长肌和腿肌进行测序,对差异表达基因进行GO功能和KEGG Pathway显著性富集分析。结果显示,180日龄苏姜猪背最长肌和90日龄苏姜猪背最长肌中有1 655个基因差异表达,其中474个基因表达上调,1 181个基因表达下调,GO功能和KEGG Pathway显著性富集分析发现,474个表达上调基因主要参与肌肉纤维、转录调节、钙信号通路、MAPK信号通路、胰岛素信号通路、FOXO信号通路等生物学功能,1 181个表达下调基因主要参与免疫、造血、溶酶体、绑定、酶活性等生物学功能;180日龄苏姜猪背最长肌和腿肌中有383个基因差异表达,其中70个基因表达上调,313个基因表达下调,GO功能和KEGG Pathway显著性富集分析发现,70个表达上调基因未能显著富集,313个表达下调基因主要参与细胞分化增殖、肌肉发育、细胞外基质、cGMP-PKG信号通路等生物学功能。本试验获得了苏姜猪背最长肌和腿肌组织的转录组信息,筛选出6个与苏姜猪肉质性状相关的候选基因:FOXO1、FOXO3、FOXO4、MYF6、A-FABPH-FABP,为深入研究苏姜猪肉质性状的分子机理奠定基础。

关键词: RNA-seq; 苏姜猪; 肉质性状; 背最长肌; 腿肌; 候选基因

Abstract: In order to obtain the genes related to meat quality traits,the transcriptomes of longissimus dorsi muscle and leg muscle of three Sujiang pigs at 90- and 180-day-old were sequenced using RNA-seq technology.The differentially expressed genes of the longissimus dorsi muscle between 180- and 90-day-old Sujiang pigs,and the differentially expressed genes between the longissimus dorsi muscle and the leg muscle of 180-day-old Sujiang pigs were analyzed with the gene ontology (GO) and KEGG Pathway strategies.The results showed that there were 1 655 differentially expressed genes between two longissimus dorsi muscle tissues at different ages.GO functional and KEGG Pathway enrichment analysis revealed that 474 up-regulated genes were mainly involved in biological functions such as muscle fiber,transcriptional regulation,calcium signaling pathway,MAPK signaling pathway,insulin signaling pathway and FOXO signaling pathway,and 1 181 down-regulated genes were mainly involved in biological functions such as immunity,hematopoiesis,lysosomes,binding,and enzyme activities.There were 383 differentially expressed genes between the longissimus dorsi muscle and leg muscle of 180-day-old Sujiang pigs.GO functional and KEGG Pathway enrichment analysis revealed that no significant results were identified in the 70 expression up-regulated genes,and 313 down-regulated genes were mainly involved in cell differentiation and proliferation,muscle development,extracellular matrix,cGMP-PKG signaling pathway and other biological functions.In this experiment,the transcriptome information of the longissimus dorsi muscle and leg muscle tissues of Sujiang pigs were obtained,and 6 candidate genes (FOXO1,FOXO3,FOXO4,MYF6,A-FABP and H-FABP) related to meat quality traits were screened.This results laid a foundation for further study of the molecular mechanism of meat quality traits in Sujiang pigs.

Key words: RNA-seq; Sujiang pig; meat quality; longissimus dorsi muscle; leg muscle; candidate gene

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