《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (7): 2038-2044.doi: 10.16431/j.cnki.1671-7236.2019.07.019

• 遗传繁育 • 上一篇    下一篇

SH2B1基因及miR-276-3p对猪背部脂肪沉积的影响

刘欣1, 巩建飞1,2, 李寒妹1,3, 颜华1, 张龙超1, 王立刚1, 赵福平1, 高红梅1, 侯欣华1, 王立贤1   

  1. 1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
    2. 青岛农业大学动物科技学院, 青岛 266109;
    3. 河北科技师范学院, 秦皇岛 066600
  • 收稿日期:2018-12-12 出版日期:2019-07-20 发布日期:2019-07-22
  • 通讯作者: 王立贤 E-mail:iaswlx@263.net
  • 作者简介:刘欣(1983-),女,山西五台人,博士,研究方向:动物遗传育种,E-mail:firstliuxin@163.com
  • 基金资助:

    北京市自然科学基金青年项目(6174047);中国农业科学院科技创新工程(ASTIP-IAS02)

Effects of SH2B1 Gene and miR-276-3p on Backfat Deposition in Pigs

LIU Xin1, GONG Jianfei1,2, LI Hanmei1,3, YAN Hua1, ZHANG Longchao1, WANG Ligang1, ZHAO Fuping1, GAO Hongmei1, HOU Xinhua1, WANG Lixian1   

  1. 1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China;
    3. Hebei Normal University of Science & Technology, Qinhuangdao 066600, China
  • Received:2018-12-12 Online:2019-07-20 Published:2019-07-22

摘要:

本试验旨在研究SH2B衔接因子蛋白1(SH2B adaptor protein 1,SH2B1)基因在猪不同组织和生长发育各阶段背部脂肪中的表达情况,预测调控该基因的miR-276-3p对猪背部脂肪表达的影响。应用实时荧光定量PCR技术检测SH2B1基因在猪脂肪、下丘脑等6种组织,以及在30、60、90、120和180 d猪背部脂肪组织中的相对表达量。靶标预测SH2B1基因的调控miRNA,并通过实时荧光定量PCR检测miR-276-3p对该基因的调控作用。结果显示,SH2B1基因在猪的6种组织中均有表达,且在脂肪组织中表达量最高,在肌肉组织中表达量最低。在猪生长发育各阶段背部脂肪中SH2B1基因均有表达,在前期(30和60 d)表达量较低,在中、后期(90、120和180 d)持续高表达,且显著高于前期表达量(P<0.05)。高、低背膘厚组背部脂肪中miR-276-3p与SH2B1基因均呈差异表达,且两者表达呈相反趋势,miR-276-3p在高背膘厚组中的表达量显著低于低背膘厚组(P<0.05),而SH2B1基因在高背膘厚组中的表达量却显著高于低背膘厚组(P<0.05)。miR-276-3p可通过靶向负调控SH2B1基因,影响猪背部脂肪的沉积。本试验结果为进一步深入研究猪背部脂肪沉积和背膘厚差异的分子机制提供参考。

关键词: 猪; SH2B1基因; miR-276-3p; 背膘厚

Abstract:

This study was aimed to investigate the expression of SH2B1 gene in the different tissues and backfat at various growth stages of pigs,and predict the effect of miR-276-3p which regulated SH2B1 gene on the expression of backfat in pigs.Real-time quantitative PCR was used to detect the relative expression of SH2B1 gene of six tissues in pigs,and in backfat of pigs at 30,60,90,120 and 180 d.The target regulation miRNA of SH2B1 gene was predicted and the regulation of SH2B1 gene by miR-276-3p was detected by Real-time quantitative PCR.The results showed that SH2B1 gene was expressed in all six tissues,with the highest expression in fat and the lowest expression in muscle.The expression of SH2B1 gene was expressed at various stages of growth and development in pigs.The expression level was low at the early stage (30 and 60 d) and high at the middle and late stages (90,120 and 180 d).The expression of SH2B1 gene was significantly higher at the middle and late stages than that at the early stage (P<0.05).The relative expression of miRNA-276-3p and SH2B1 gene in backfat tissue of pigs that in high or low backfat thickness group (HBFT or LBFT) was detected by Real-time quantitative PCR.The results showed that miRNA-276-3p and SH2B1 gene were differentially expressed in backfat tissue of pigs.The expression of miRNA-276-3p in high backfat thickness group was significantly lower than that in low backfat thickness group (P<0.05),while the expression of SH2B1 gene in high backfat thickness group was significantly higher than that in low backfat thickness group (P<0.05).miRNA-276-3p negatively regulated SH2B1 gene and affected the fat deposition on the back of pigs.This results provided a reference for further studying the molecular mechanism of fat deposition and backfat thickness difference in pigs.

Key words: pig; SH2B1 gene; miRNA-276-3p; backfat thickness

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