威宁绵羊SOCS7和GFAP基因组织表达研究

doi:10.16431/j.cnki.1671-7236.2019.07.003

摘要/Abstract

摘要：

试验旨在检测细胞因子信号传导抑制蛋白7（suppressor of cytokine signaling 7，SOCS7）和胶质纤维酸性蛋白（glial fibrillary acidic protein，GFAP）基因在威宁绵羊不同组织中的表达水平。以6月龄、1周岁和2周岁的威宁绵羊公、母羊为研究对象，采用实时荧光定量PCR测定威宁绵羊不同性别及不同生长阶段心脏、肝脏、脾脏、肺脏、肾脏及脑6个组织中SOCS7和GFAP基因的相对表达量，从mRNA水平上的探究两个基因之间的表达规律。结果显示，SOCS7基因在威宁绵羊不同组织中均有不同程度的表达，其中脾脏中相对表达量最高，其次是肺脏、肾脏、心脏、肝脏和脑组织，随着威宁绵羊年龄的增大，SOCS7基因在组织中的相对表达量呈现小幅度上升的趋势；GFAP基因在威宁绵羊脑组织中相对表达量最高，其余组织中表达量较低，随着年龄增长总体呈现下降趋势。从性别差异来看，威宁绵羊SOCS7基因相对表达量母羊普遍高于公羊，而GFAP基因则是公羊普遍高于母羊，推测SOCS7基因很有可能负向调控GFAP基因的表达。

关键词: 威宁绵羊; SOCS7基因; GFAP基因; 实时荧光定量PCR; 组织表达

Abstract:

This study was aimed to detect the expression of suppressor of cytokine signaling 7 (SOCS7) and glial fibrillary acidic protein (GFAP) genes in different tissues of Weining sheep.This experiment took male and female Weining sheep aged 6 months,1 year and 2 years as research objects,the relative expression of SOCS7 and GFAP genes in six tissues of heart,liver,spleen,lung,kidney and brain at different sex and growth stages were determined by Real-time quantitative PCR,and the mRNA level expression pattern of SOCS7 and GFAP genes were explored.The results showed that SOCS7 gene was expressed in different tissues of Weining sheep,the expression of spleen was the highest,followed by lung,kidney,heart,liver and brain,and the relative expression of SOCS7 gene in Weining sheep slightly increased with age.The relative expression of GFAP gene was the highest in brain of Weining sheep,while the rest of the tissues showed a low expression level,which showed a downward trend with age.According to the genders difference,the relative expression of SOCS7 gene in female was generally higher than male,while that of GFAP gene in male was generally higher than female,it suggested that SOCS7 gene might negatively regulate the expression of GFAP gene.

Key words: Weining sheep; SOCS7 gene; GFAP gene; Real-time quantitative PCR; tissue expression

中图分类号:

Q786

张继, 易鸣, 程朝友, 程均华, 付正仙, 杨德文, 吴雪, 邱淦远, 刘若余. 威宁绵羊SOCS7和GFAP基因组织表达研究[J]. 《中国畜牧兽医》, 2019, 46(7): 1899-1906.

ZHANG Ji, YI Ming, CHENG Chaoyou, CHENG Junhua, FU Zhengxian, YANG Dewen, WU Xue, QIU Ganyuan, LIU Ruoyu. Study on Tissue Expression of SOCS7 and GFAP Genes in Weining Sheep[J]. , 2019, 46(7): 1899-1906.

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