一例貉大肠杆菌与血虫混合感染的诊断鉴定

doi:10.16431/j.cnki.1671-7236.2019.05.035

《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (5): 1551-1557.doi: 10.16431/j.cnki.1671-7236.2019.05.035

一例貉大肠杆菌与血虫混合感染的诊断鉴定

栾明明^1,2, 王晓旭², 贾赟³, 赵家平², 张林波¹, 徐超², 韩鹏¹

1. 吉林农业大学生命科学学院, 长春 130118;
2. 中国农业科学院特产研究所, 吉林省特种经济动物分子生物学省部共建国家重点实验室, 长春 130112;
3. 辽宁省出入境检验检疫局, 大连 116001

收稿日期:2018-11-20 出版日期:2019-05-20 发布日期:2019-05-20
通讯作者: 徐超, 韩鹏 E-mail:xuchao@caas.cn;hanpengjlau@163.com
作者简介:栾明明(1992-),男,山东诸城人,硕士,研究方向:病原微生物,E-mail:790635101@qq.com
基金资助:
中国科协青年人才托举工程（2017QNRC001）；吉林省优秀青年基金（20180520032JH）；中央公益性科研院所基本科研业务费；吉林省教育厅"十三五"科学技术项目（JJKH20180674KJ）；吉林农业大学博士启动基金（2015015）

Diagnosis and Identification of a Mixed Infection of Escherichia coli and Eperythrozoon in Raccoon Dog

LUAN Mingming^1,2, WANG Xiaoxu², JIA Yun³, ZHAO Jiaping², ZHANG Linbo¹, XU Chao², HAN Peng¹

1. College of Life Science, Jilin Agricultural University, Changchun 130118, China;
2. State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China;
3. Liaoning Entry Exit Inspection and Quarantine Bureau, Dalian 116001, China

Received:2018-11-20 Online:2019-05-20 Published:2019-05-20

摘要/Abstract

摘要：

为诊断吉林养殖场一例病死貉的病原，本试验运用细菌培养、染色镜检、生化鉴定、分子生物学鉴定和血液涂片镜检等方法对采集的病死貂的肺脏、肝脏、脾脏、肾脏、肠道及血液进行病原分离鉴定，并对分离到的病原菌进行致病性和药敏试验测定。结果显示，共分离出1株优势菌株，该分离菌株在光学显微镜下呈现为两端钝圆的杆状革兰氏阴性菌；生化试验结果显示，其能分解葡萄糖、乳糖、甘露醇、麦芽糖和蔗糖产酸产气，对于M-R试验、吲哚试验、淀粉水解试验结果为阳性；其16S rRNA序列与NCBI数据库中登录的已知大肠杆菌序列同源性达99%；动物致死性试验结果表明，该菌株对小鼠具有致病性；药敏试验结果显示，分离菌对喹诺酮类药物诺氟沙星和环丙沙星最敏感，对其他药物具有不同程度的耐药性；对病貉抗凝血进行涂片镜检证明有血虫感染，且红细胞感染率达98%以上。综上所述，该病貉感染的病原菌株为大肠杆菌且该大肠杆菌具有致病性，同时病貉患有血虫病，并导致了其生长发育迟缓。

关键词: 貉; 血虫体; 大肠杆菌; 诊断鉴定

Abstract:

To diagnose the pathogen of a diseased racoon dog in a Jilin farm,in this experiment,bacterial culture,staining microscopy,biochemical identification,molecular biology identification and blood smear microscopy were used to isolate and identify the pathogens of the collected lungs,liver,spleen,kidney,intestines and blood.And the isolated pathogens were tested for pathogenicity and susceptibility test.The results showed that a dominant strain was isolated,which was presented as a rod-shaped Gram-negative bacilli with obtuse ends at the optical microscope.The results of biochemical tests showed that it could decompose glucose-lactose,mannitol,maltose and sucrose to produce acid gas,which was positive for M-R test,sputum test and starch hydrolysis test.The 16S rRNA sequence had 99% homology with the known E.coli sequence registered in the NCBI database.The animal lethality test results showed that the strain was pathogenic to mice.The results of drug susceptibility test showed that the isolate was most sensitive to the quinolone drugs norfloxacin and ciprofloxacin,and had different degrees of resistance to other drugs.The anti-coagulation smears of sick racoon dog proved to be infected by Eperythrozoon,and the infection rate of red blood cells reached more than 98%.In summary,the pathogenic strain of the disease infection was E.coli and the E.coli was pathogenic,at the same time,the sick raccoon dog suffered from Eperythrozoon and led to its growth retardation.

Key words: racoon dog; Eperythrozoon; Escherichia coli; diagnosis and identification

中图分类号:

S858.92

栾明明, 王晓旭, 贾赟, 赵家平, 张林波, 徐超, 韩鹏. 一例貉大肠杆菌与血虫混合感染的诊断鉴定[J]. 《中国畜牧兽医》, 2019, 46(5): 1551-1557.

LUAN Mingming, WANG Xiaoxu, JIA Yun, ZHAO Jiaping, ZHANG Linbo, XU Chao, HAN Peng. Diagnosis and Identification of a Mixed Infection of Escherichia coli and Eperythrozoon in Raccoon Dog[J]. , 2019, 46(5): 1551-1557.

参考文献

[1] 刘君君,卢亚楠,金礼吉,等.中国毛皮动物养殖现状及发展趋势[J].中国皮革,2007,11:18-21. LIU J J,LU Y N,JIN L J,et al.Present situation and development of China fur animal feeding[J].China Leather,2007,11:18-21.(in Chinese)
[2] 任战军,叶翔杨,宫瑞光,等.现代毛皮动物养殖业发展方向与关键技术[J].经济动物学报,2016,20(2):63-68. REN Z J,YE X Y,GONG R G,et al.Industrial development direction and key technology of the modern fur animal breeding[J].Journal of Economic Animal,2016,20(2):63-68.(in Chinese)
[3] 崔秀花.毛皮动物腹泻病的防治新策略[J].北方牧业,2012,14:25. CUI X H.New strategy for prevention and treatment of diarrhea in fur animals[J].Northern Animal Husbandry,2012,14:25.(in Chinese)
[4] 李胜杰.狐、貉、貂腹泻的原因及防治措施[J].中国畜禽种业,2014,10(6):81. LI S J.Causes and control measures of diarrhea in foxes,minks and minks[J].The Chinese Livestock and Poultry Breeding,2014,10(6):81.(in Chinese)
[5] 王贵升.水貂腹泻病的流行病学调查和主要病原分子生物学特点分析[D].济南:山东大学,2018. WANG G S.Epidemiological investigation and analysis of molecular biological characteristics of main pathogens of mink diarrhea[D].Jinan:Shandong University,2018.(in Chinese)
[6] 戴益民,廖财智,赖芬菊.一例致病性大肠杆菌的分离鉴定及诊治[J].江西农业大学学报,2018,5:1048-1054. DAI Y M,LIAO C Z,LAI F J.Isolation,identification and diagnosis of a case of pathogenic Escherichia coli[J].Acta Agriculturae Universitatis Jiangxiensis,2018,5:1048-1054.(in Chinese)
[7] 孙娜,陈强,杨艳玲,等.水貂志贺氏菌分离鉴定及药物敏感性分析[J].中国畜牧兽医,2017,44(11):3379-3384. SUN N,CHEN Q,YANG Y L,et al.Isolation,identification and drug sensitivity analysis of Shigella from minks[J].China Animal Husbandry & Veterinary Medicine,2017,44(11):3379-3384.(in Chinese)
[8] RUDI K,HØIDAL H K,KATLA T,et al.Direct Real-time PCR quantification of Campylobacter jejuni in chicken fecal and cecal samples by integrated cell concentration and DNA purification[J].Applied & Environmental Microbiology,2004,70(2):790-797.
[9] 曹雪峰,彭广能,黄祥明,等.腹泻犬中细小病毒携带情况以及VP2基因进化分析[J].病毒学报,2016,32(6):733-739. CAO X F,PENG G N,HUANG X M,et al.Canine parvovirusin diarrheal dogs and analyses of the full-length VP2 gene of dogs[J].Chinese Journal of Virology,2016,32(6):733-739.(in Chinese)
[10] 郑龙龙,朱玲云,陈关雄,等.断奶仔猪腹泻病因分析[J].中国畜牧兽医,2015,42(10):2772-2778. ZHENG L L,ZHU L Y,CHEN G X,et al.Causes analysis of weaning piglets diarrhea[J].China Animal Husbandry & Veterinary Medicine,2015,42(10):2772-2778.(in Chinese)
[11] 史秋梅,张艳英,房海,等.腹泻水貂检出携带耶尔森菌HPI毒力岛的大肠杆菌[J].中国兽医学报,2012,32(6):857-861. SHI Q M,ZHANG Y Y,FANG H,et al.Detection of pathogenicity islands of Yersinia enterocolitica HPI from Escherichia coli strains in diarrheic minks[J].Chinese Journal of Veterinary Science,2012,32(6):857-861.(in Chinese)
[12] 王旭荣,王国庆,张景艳,等.致病性奇异变形杆菌和大肠杆菌混合感染致奶牛关节脓肿和腹泻的研究[J].中国畜牧兽医,2013,40(9):195-198. WANG X R,WANG G Q,ZHANG J Y,et al.Study on mix infection of pathogenic Proteus mirabillis and E.coli causing joint abscess and diarrhea of dairy cows[J].China Animal Husbandry & Veterinary Medicine,2013,40(9):195-198.(in Chinese)
[13] 钟世勋,迟珊珊,王振,等.山东规模化养殖场毛皮动物多重感染病原学分析[J].中国兽医学报,2014,34(11):1770-1777. ZHONG S X,CHI S S,WANG Z,et al.The etiology analysis of multiple infections in Shandong scale fur farms[J].Chinese Journal of Veterinary Science,2014,34(11):1770-1777.(in Chinese)
[14] 姚火春.兽医微生物学实验指导[M].北京:中国农业出版社,2012. YAO H C.Instruction for Veterinary Microbiology Experiments[M].Beijing:China Agriculture Press,2012.(in Chinese)
[15] 张英曼.1例仔貉附红细胞体病的诊治[J].养殖与饲料,2014,1:56-57. ZHANG Y M.Diagnosis and treatment of Eperythrozoonosis in a young raccoon[J].Animals Breeding and Feed,2014,1:56-57.(in Chinese)
[16] COOKE E M.Escherichia coli-An overview[J].Journal of Hygiene,1985,95(3):523.
[17] 马兴树,范翠蝶,夏玉龙.禽致病性大肠杆菌研究进展[J].中国畜牧兽医,2013,40(2):169-174. MA X S,FAN C D,XIA Y L.Research progress on avian pathogenic Escherichia coli[J].China Animal Husbandry & Veterinary Medicine,2013,40(2):169-174.(in Chinese)
[18] 邹玲,唐栋,刘文华,等.鸡大肠杆菌病病原的分离鉴定及药敏试验[J].中国畜牧兽医,2007,34(2):119-121. ZOU L,TANG D,LIU W H,et al.The separation appraisal and drugs susceptibility test of chicken's Escherichia coli disease etiology[J].China Animal Husbandry & Veterinary Medicine,2007,34(2):119-121.(in Chinese)
[19] 张艳英,史秋梅,房海,等.腹泻仔貉检出携带耶尔森菌HPI毒力岛的大肠杆菌[J].中国人兽共患病学报,2012,28(2):179-182. ZHANG Y Y,SHI Q M,FANG H,et al.Detection on pathogenicity islands of Yersinia enterocolitica HPI from Escherichia coli strains in diarrheic young raccoon dogs[J].Chinese Journal of Zoonosis,2012,28(2):179-182.(in Chinese)
[20] JIANG H X,LYU D H,CHEN Z L,et al.High prevalence and widespread distribution of multi-resistant Escherichia coli isolates in pigs and poultry in China[J].Veterinary Journal,2011,187(1):99-103.
[21] 冯涛,薛原.貉源大肠杆菌耐药性的检测[J].野生动物学报,2017,38(3):498-500. FENG T,XUE Y.Analysis of antimicrobial resistance in Escherichia coli from raccoon dog (Nyctereutes procyonoides)[J].Chinese Journal of Wildlife,2017,38(3):498-500.(in Chinese)
[22] 魏梅雄,马杏宝,王龙英.附红细胞体病及其分子生物学和发病机制的研究进展[J].旅行医学科学,2006,12(3):1-7. WEI M X,MA X B,WANG L Y.Recently development on eperythrozoonosis and its molecular biology and the disease mechanism[J].Travel Medicine Science,2006,12(3):1-7.(in Chinese)
[23] 张振兴,李玉峰.嗜血支原体病研究进展[J].畜牧与兽医,2013,45(12):1-5. ZHANG Z X,LI Y F.Research progress of hemophilic mycoplasmosis[J].Animal Husbandry & Veterinary Medicine,2013,45(12):1-5.(in Chinese)
[24] 宋晓东,宋月峰.貉的养殖管理技术[J].中国动物保健,2013,15(3):63-65. SONG X D,SONG Y F.Racoon breeding management technology[J].China Animal Health,2013,15(3):63-65.(in Chinese)

一例貉大肠杆菌与血虫混合感染的诊断鉴定

Diagnosis and Identification of a Mixed Infection of Escherichia coli and Eperythrozoon in Raccoon Dog

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