日粮中添加藤茶提取物对猪生长性能、血液生化指标、抗氧化活性的影响

doi:10.16431/j.cnki.1671-7236.2019.05.010

《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (5): 1330-1339.doi: 10.16431/j.cnki.1671-7236.2019.05.010

日粮中添加藤茶提取物对猪生长性能、血液生化指标、抗氧化活性的影响

熊云霞, 王丽, 温晓鹿, 胡友军, 易宏波, 邱月琴, 杨雪芬, 吴绮雯, 蒋宗勇

广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 农业部华南动物营养与饲料重点实验室, 广东省动物育种与营养公共实验室, 广东省畜禽育种与营养研究重点实验室, 广州 510640

收稿日期:2018-10-31 出版日期:2019-05-20 发布日期:2019-05-20
通讯作者: 王丽 E-mail:wangli1@gdaas.cn
作者简介:熊云霞(1988-),女,江西南昌人,助理研究员,研究方向:猪营养与饲料科学,E-mail:xiangtang.2000@163.com
基金资助:
国家"十三五"重点研发专项（2016YFD0500501、2018YFD0501101）；国家生猪产业技术体系建设专项（CARS-35）；省级现代农业（畜禽健康养殖）产业技术研发中心建设项目

Effects of adding Ampelopsis grossedentata Extract in Diets on Growth Performance, Blood Biochemical Indexes and Antioxidant Activity in Pig

XIONG Yunxia, WANG Li, WEN Xiaolu, HU Youjun, YI Hongbo, QIU Yueqin, YANG Xuefen, WU Qiwen, JIANG Zongyong

State Key Laboratory of Livestock and Poultry Breeding, Guangdong Public Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received:2018-10-31 Online:2019-05-20 Published:2019-05-20

摘要/Abstract

摘要：

为研究日粮中添加藤茶提取物对不同体重阶段猪生长性能、血液生化指标、抗氧化活性的影响，本研究选用平均初始体重为30 kg的三元杂交（杜×长×大）去势公猪90头，随机分成3个处理，分别为对照组（CON组，饲喂基础日粮）、植物精油复合物组（A组，基础日粮中添加0.03%植物精油复合物）和藤茶提取物组（B组，基础日粮中添加0.03%藤茶提取物），根据不同体重阶段（30~50、50~75、75~100 kg及100 kg~出栏）饲喂不同阶段的基础日粮，每次换料前及试验结束时称重采血，分析不同体重阶段添加0.03%藤茶提取物对猪生长性能、血液生化指标、抗氧化活性的影响。结果表明：与CON组相比，添加0.03%藤茶提取物显著提高了30~75 kg猪的平均日采食量（ADFI）和平均日增重（ADG）（P<0.05）；显著降低了30~50 kg猪血清乳酸（LA）含量（P<0.05）；显著提高了50~75 kg猪血液胰岛素（INS）浓度（P<0.05），但对血糖（GLU）浓度无显著影响（P>0.05）；显著提高了75~100 kg猪GLU浓度（P<0.05），INS浓度有升高趋势（P>0.05），乳酸脱氢酶（LDH）含量显著降低（P<0.05）；各体重阶段血清生长激素（GH）含量均有升高趋势（P>0.05）。B组猪血清总超氧化物岐化酶（T-SOD）活力、丙二醛（MDA）含量、谷胱甘肽过氧化物酶（GSH-Px）活力、总抗氧化能力（TAOC）在各体重阶段与CON组相比均无显著差异（P>0.05）。与A组相比，添加0.03%藤茶提取物显著提高了30~100 kg猪的ADFI （P<0.05），显著降低了30~50 kg猪血清LA含量（P<0.05），且显著提高了50 kg以上体重阶段猪血清GSH-Px活力（P<0.05）。综合试验结果，添加0.03%藤茶提取物可促进30~75 kg猪生长代谢，在一定程度改善50~100 kg猪的血糖代谢，且添加藤茶提取物效果优于添加等量植物精油复合物。

关键词: 藤茶提取物; 猪; 生长性能; 血液生化指标; 抗氧化活性

Abstract:

In order to study the effects of Ampelopsis grossedentata extract on growth performance,blood biochemical indexes and antioxidant activities in pigs at different weight stages,ninety crossbred (Duroc×Landrace×Yorkshire) castrated boars with an average initial body weight of 30 kg were randomly divided into three groups,CON group (fed with basal diet),group A (fed with basal diet adding 0.03% plant essential oil complex)and group B (fed with basal diet adding 0.03% Ampelopsis grossedentata extract),respectively.Pigs were fed with different basic diets according to body weight stages (30-50,50-75,75-100 kg and 100 kg-out).Pigs were weighted and blood samples were collected before and after each change of basic diets to analysis the effects of 0.03% Ampelopsis grossedentata extract on the growth performance,blood biochemical parameters and antioxidant activity of different weight stages pigs.The results showed that:Compared with the CON group,adding 0.03% Ampelopsis grossedentata extract significantly increased ADFI and ADG of the 30-75 kg pigs (P<0.05).The content of blood lactic acid (LA) was significantly decreased at 30-50 kg body weight (P<0.05).At 50-75 kg,the insulin (INS) concentration in blood was significantly increased (P<0.05),but there was no significant difference in blood glucose (GLU) concentration (P>0.05).At 75-100 kg,GLU concentration was significantly elevated (P<0.05),meanwhile there was an increased trend for INS concentration (P>0.05),and the content of lactate dehydrogenase (LDH) was significantly decreased (P<0.05).There was an increased trend for serum growth hormone (GH) content at each weight stage (P>0.05).Serum T-SOD activity,MDA content,GSH-Px activity,and TAOC were not significantly different compared with the CON group at any weight stage (P>0.05).Compared with the plant essential oil complex group,addition 0.03% Ampelopsis grossedentata extract significantly increased the ADFI of the pigs at 30-100 kg body weight stage (P<0.05),and significantly reduced the content of LA of 30-50 kg pigs (P<0.05),and significantly increased the GSH-Px activity at the weight of 50 kg or more (P<0.05).In conclusion,adding 0.03% Ampelopsis grossedentata extract could promote the growth and metabolism of pigs at 30-75 kg,and improve the carbohydrate metabolism of pigs at 50-100 kg body weight to some extent,and the effect of adding Ampelopsis grossedentata extract was superior to the plant essential oil complex at the same amount.

Key words: Ampelopsis grossedentata extract; pigs; growth performance; blood biochemical indexes; antioxidant activity

中图分类号:

S816.7

熊云霞, 王丽, 温晓鹿, 胡友军, 易宏波, 邱月琴, 杨雪芬, 吴绮雯, 蒋宗勇. 日粮中添加藤茶提取物对猪生长性能、血液生化指标、抗氧化活性的影响[J]. 《中国畜牧兽医》, 2019, 46(5): 1330-1339.

XIONG Yunxia, WANG Li, WEN Xiaolu, HU Youjun, YI Hongbo, QIU Yueqin, YANG Xuefen, WU Qiwen, JIANG Zongyong. Effects of adding Ampelopsis grossedentata Extract in Diets on Growth Performance, Blood Biochemical Indexes and Antioxidant Activity in Pig[J]. , 2019, 46(5): 1330-1339.

参考文献

[1] 王岩,周莉玲,李锐,等.显齿蛇葡萄化学成分的研究[J].中药材,2002,25(4):254-256. WANG Y,ZHOU L L,LI R,et al.Study on the chemical fromm Ampelopsis grossedentata[J].Journal of Chinese Medicinal Materials,2002,25(4):254-256.(in Chinese)
[2] WANG Y F,BIAN X Y,PARK J,et al.Physicochemical properties,in vitro antioxidant activities and inhibitory potential against alpha-glucosidase of polysaccharides from Ampelopsis grossedentata leaves and stems[J].Molecules,2011,16(9):7762-7772.
[3] 杨娟,赵炳超,于倩倩,等.新型饲料添加剂藤茶黄酮的研究进展[J].中国畜牧兽医,2011,38(4):47-51. YANG J,ZHAO B C,YU Q Q,et al.Research progress in new feed additives Ampelopsis grossedentata flavonoids[J].China Animal Husbandry & Veterinary Medicine,2011,38(4):47-51.(in Chinese)
[4] 钟礼云,林蔚,林健,等.藤茶水提取物的长期毒性试验研究[J].海峡预防医学杂志,2012,18(4):46-48. ZHONG L Y,LIN W,LIN J,et al.Long-term toxicity test of vine tea extract[J].Strait Journal of Preventive Medicine,2012,8(4):46-48.(in Chinese)
[5] 何子煜,伍树松,贺喜.藤茶的生理功能及其在畜禽生产中的应用[J].中国饲料,2018,15:78-84. HE Z Y,WU S S,HE X.Biological function of vine tea and its application in animal production[J].China Feed,2018,15:78-84.(in Chinese)
[6] WU Y P,BAI J R,ZHONG K,et al.A dual antibacterial mechanism involved in membrane disruption and DNA binding of 2R,3R-dihydromyricetin from pine needles of Cedrus deodara against Staphylococcus aureus[J].Food Chemistry,2017,218(15):463-470.
[7] WU Y P,BAI J R,ZHONG K,et al.Antibacterial effect of 2R,3R-dihydromyricetin on the cellular functions of Staphylococcus aureus[J].Bioscience Biotechnology Biochemistry,2018,82(1):135-138.
[8] HOU X,ZHANG J,AHMAD H,et al.Evaluation of antioxidant activities of Ampelopsin and its protective effect in lipopolysaccharide-induced oxidative stress piglets[J].PLoS One,2014,9(9):e108314.
[9] HOU X L,TONG Q,WANG W Q,et al.Suppression of inflammatory responses by dihydromyricetin,a flavonoid from Ampelopsis grossedentata,via inhibiting the activation of NF-κB and MAPK signaling pathways[J].Journal of Natural Products,2015,78(7):1689-1696.
[10] WENG L H,ZHANG H,LI X,et al.Ampelopsin attenuates lipopolysaccharide-induced inflammatory response through the inhibition of the NF-kappa B and JAK2/STAT3 signaling pathways in microglia[J].International Immunopharmacology,2017,44(1):1-8.
[11] LU C J,HE Y F,YUAN W Z,et al.Dihydromyricetin-mediated inhibition of the Notch1 pathway induces apoptosis in QGY7701 and HepG2 hepatoma cells[J].World Journal of Gastroenterology,2017,23(34):6242-6251.
[12] 戴青.藤茶二氢杨梅素对肉鸡促生长作用研究[D].贵阳:贵州大学,2015. DAI Q.Study on the growth promoting effect of dihydromyricetin from Ampelopsis grossedentata on chickens[D].Guiyang:Guizhou University,2015.(in Chinese)
[13] 武书庚,刁其玉,石波,等.藤茶黄酮对仔猪生产性能的影响[J].饲料工业,2005,26(7):29-31. WU S G,DIAO Q Y,SHI B,et al.Effect of vine tea flavonoids on performance of piglets[J].Feed Industry,2005,26(7):29-31.(in Chinese)
[14] 杨茂林,李明祖,刘若余.生态复合藤茶饲料添加剂对江口萝卜猪育肥性能及抗病力的影响[J].贵州畜牧兽医,2014,38(5):11-13. YANG M L,LI M Z,LIU R Y.Effect of ecological composite Ampelopsis grossedentata feed additive on the fattening performance and disease resistance of Jiangkou radish pig[J].Guizhou Journal of Aniaml Husbandry & Veterinary Medicine,2014,38(5):11-13.(in Chinese)
[15] 赵萌,罗杰,郁建生,等.藤茶总黄酮对仔猪生长性能的影响[J].饲料工业,2015,36(20):14-17. ZHAO M,LUO J,YU J S,et al.Effects of total flavone from Ampelopsis grossedentata on growth performance of piglet[J].Feed Industry,2015,36(20):14-17.(in Chinese)
[16] 陈丽,孙云子.藤茶提取物对小鼠生长性能、腹泻率及抗氧化性能的影响[J].中国畜牧兽医,2017,44(4):1027-1031. CHEN L,SUN Y Z.Effect of Ampelopsis grossedentata extract on growth performance,diarrhea rate and anti-oxidation function in mice[J].China Animal Husbandry & Veterinary Medicine,2017,44(4):1027-1031.(in Chinese)
[17] 郭航,王永军,谢鹏,等.日粮添加二氢杨梅素对肉仔鸡肠黏膜形态结构、碱性磷酸酶及生产性能的影响[J].中国饲料,2008,6:19-22. GUO H,WANG Y J,XIE P,et al.Effects of addition dihydromyriceton intestinal mucosal morphology of broilers structure,alkaline phosphatase and production performance[J].China Feed,2008,6:19-22.(in Chinese)
[18] 郭航,谢鹏,张敏红,等.短期灌服二氢杨梅素对受损肉仔鸡肠黏膜的修复及免疫机能的影响[J].中国兽医杂志,2010,46(4):19-22. GUO H,XIE P,ZHANG M H,et al.Effects of short-term administration of dihydromyricetin on damaged broilers intestinal mucosa and the repair and immune machinism[J].Chinese Journal of Veterinary Medicine,2010,46(4):19-22.(in Chinese)
[19] 杨家军.早期断奶仔猪肠上皮细胞氧化与损伤及二氢杨梅素对其调控作用[D].北京:中国农业科学院,2012. YANG J J.The oxidation and impairment of piglets'intestinal epithelium cell caused by early-weaning and its regulation of supplemented dihydromyricetin[D].Beijing:Chinese Academy of Agricultural Sciences,2012.(in Chinese)
[20] MURAKAMI T,MIYAKOSHI M,ARAHO D,et al.Hepatoprotective activity of tocha,the stems and leaves of Ampelopsis grossedentata,and ampelopsin[J].Biofactors,2004.21(1-4):175-178.
[21] 赵萌.藤茶总黄酮对仔猪生长性能的影响研究[D].贵阳:贵州大学,2016. ZHAO M.Effects of total flavone from Ampelopsis grossedentata on growth performance of piglet[D].Guiyang:Guizhou University,2016.(in Chinese)
[22] 刘畅,邓薇,刘小英,等.藤茶生物活性成分及其制备工艺研究进展[J].食品工业,2015,36(4):233-237. LIU C,DENG W,LIU X Y,et al.Research advances on active components and preparation technology of Ampelopsis grossedentata[J].Food Industry,2015,36(4):233-237.(in Chinese)
[23] WANG Y F,YING L,SUN D,et al.Supercritical carbon dioxide extraction of bioactive compounds from Ampelopsis grossedentata stems:Process optimization and antioxidant activity[J].International Journal of Molecular Sciences,2011,12(10):6856-6870.
[24] 韩爱云.二氢杨梅素对肉仔鸡免疫器官发育、血液生化指标及肝细胞DNA损伤的影响[D].保定:河北农业大学,2006. HAN A Y.Effect of dihydromyricetin on immune organ growth,serum biochemical papameters and DNA damage in hepatocyte of broilers[D].Baoding:Hebei Agricultural University,2006.(in Chinese)
[25] 赵萌,郁建生,郁建平,等.藤茶总黄酮对仔猪血清生化指标及免疫功能的影响[J].中国畜牧兽医,2016,43(5):1221-1225. ZHAO M,YU J S,YU J P.et al.Effect of Ampelopsis grossedentata flavonoids onserum biochemical indexes and immune function of piglets[J]. China Animal Husbandry & Veterinary Medicine,2016,43(5):1221-1225.(in Chinese)
[26] 漆姣媚,蒋燕群,张杰,等.显齿蛇葡萄总黄酮降血糖作用研究[J].中国药学杂志,2017,52(19):1685-1690. QI J M,JIANG Y Q,ZHANG J,et al.Hypoglycemic effect of total flavonoids from Ampelopsis grossedentata[J].Chinese Pharmaceutical Journal,2017,52(19):1685-1690.(in Chinese)
[27] CHEN S H,ZHAO X L,WAN J,et al.Dihydromyricetin improves glucose and lipid metabolism and exerts anti-inflammatory effects in nonalcoholic fatty liver disease:A randomized controlled trial[J].Pharmacological Research,2015,99(4):74-81.
[28] WAN W T,JIANG B P,SUN L,et al.Metabolomics reveals that vine tea (Ampelopsis grossedentata) prevents high-fat-diet-induced metabolism disorder by improving glucose homeostasis in rats[J].PLoS One,2017,12(8):e0182830.
[29] 梁萌.藤茶提取物对肉鸡免疫性能、脂质过氧化水平和脂类代谢的影响[D].泰安:山东农业大学,2004. LIANG M.Effects of extracts from Ampelopsis grossedentata on the immune function,antioxidant and lipid metabolism of broiler chickens[D].Tai'an:Shandong Agricultural University,2004.(in Chinese)
[30] 陈玉琼.藤茶中黄酮、二氢杨梅素的提取分离、降血脂作用及藤茶安全评价的研究[D].武汉:华中农业大学,2007. CHEN Y Q.Study on extraction,isolation and hypolipidemic effects of flavones and dihydromyricetin from Tengcha and safty assessments of Tengcha[D].Wuhan:Huazhong Agricultural University,2007.(in Chinese)
[31] 陈玉琼,倪德江,程倩,等.藤茶总黄酮及二氢杨梅素降血脂作用研究[J].茶叶科学,2007,3:221-225. CHEN Y Q,NI D J,CHENG Q,et al.Study on the hypolipidemic effect of flavones and dihydromyricetin from Tengcha[J].Journal of Tea Science,2007,3:221-225.(in Chinese)
[32] 杨志秋.藤茶提取物对小鼠肥胖的预防及治疗效果的研究[D].杭州:浙江工业大学,2011. YANG Z Q.The preventive and therapeutic effect of Ampelopsis grossedentata extracts from on dite-induced obese mice[D].Hangzhou:Zhejiang University of Technology,2011.(in Chinese)
[33] ZHANG Y S,NING Z X,YANG S Z,et al.Antioxidation properties and mechanism of action of dihydromyricetin from Ampelopsis grossedentata[J].Acta Pharmaceutica Sinica,2003,38(4):241-244.
[34] GAO J H,LIU B G,NING Z X,et al.Characterization and antioxidant activity of flavonoid-rich exacts from leaves of Ampelopsis grossedentata[J].Journal of Food Biochemistry,2010,33(6):808-820.
[35] LIAO W Z,NING Z X,MA L,et al.Recrystallization of dihydromyricetin from Ampelopsis grossedentata and its anti-oxidant activity evaluation[J].Rejuvenation Research,2014,17(5):422-429.
[36] ZHENG X J,XIAO H,ZENG Z,et al.Composition and serum antioxidation of the main flavonoids from fermented vine tea (Ampelopsis grossedentata)[J].Journal of Functional Foods,2014,9(1):290-294.
[37] 唐瑛,罗祖友,严奉伟,等.藤茶总黄酮对小鼠的体内外抗氧化作用研究[J].中国医院药学杂志,2006,26(12):1449-1452. TANG Y,LUO Z Y,YAN F W,et al.Study on the anti-oxidative effect of total flavonoids from Ampelopsis grossedentata (AGTF) in vivo and in vitro[J].Chinese Journal of Hospital Pharmacy,2006,26(12):1449-1452.(in Chinese)
[38] TAN G Y,ZHANG M H,FENG J H,et al.Effects of pretreatment by the flavanol Ampelopsin on porcine kidney epithelial cell injury induced by hydrogen peroxide[J].Agricultural Sciences in China,2010,9(4):598-604.

日粮中添加藤茶提取物对猪生长性能、血液生化指标、抗氧化活性的影响

Effects of adding Ampelopsis grossedentata Extract in Diets on Growth Performance, Blood Biochemical Indexes and Antioxidant Activity in Pig

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	陈楷文, 赵云娇, 王磊, 张剑搏, 马玉红, 吴国芳. 不同氧浓度下猪睾丸间质细胞转录组分析[J]. 中国畜牧兽医, 2023, 50(6): 2185-2195.
[2]	韦明邦, 边巴琼达, 肖青青, 段梦琪, 强巴央宗, 商鹏. 藏猪CDKN1B基因克隆、生物信息学及表达分析[J]. 中国畜牧兽医, 2023, 50(6): 2196-2206.
[3]	李杨辉, 许涛, 张淑贤, 刘珂, 贾天晴, 陈宁, 尹君亮, 席琳乔, 王栋, 周小玲. 孜然秸秆替代小麦秸秆对羔羊生长和屠宰性能及血浆生化指标的影响[J]. 中国畜牧兽医, 2023, 50(6): 2296-2304.
[4]	吴立婷, 陆睿, 刘半红, 包红朵, 王永娟, 周艳, 王冉, 张辉. 产气荚膜梭菌噬菌体及乳酸杆菌联用改善肉鸡生长性能和调节肠道菌群功能研究[J]. 中国畜牧兽医, 2023, 50(6): 2321-2332.
[5]	张方玮, 张琪, 雷亮亮, 孙武胜, 张迪, 张云鹏, 张净搏, 王修权, 张晶, 张树敏. 松辽黑猪HBEGF基因多态性及其与繁殖性状的关联分析[J]. 中国畜牧兽医, 2023, 50(6): 2370-2379.
[6]	孙守湖, 陈志飞, 郭智轩, 俞婷, 王修武, 李松倍, 魏文康, 贺东生. 3种猪冠状病毒N基因密码子偏好性及宿主适应机制研究[J]. 中国畜牧兽医, 2023, 50(6): 2403-2413.
[7]	曹琪, 金清, 袁意, 冯逸雪, 孔丹妮, 陈翔鸿, 吴斌, 宋文博, 汤细彪. 规模化猪场常见呼吸道细菌性病原的流行病学调查[J]. 中国畜牧兽医, 2023, 50(6): 2439-2449.
[8]	史秋颖, 段宝敏, 李颖, 牛登云, 耿晨阳, 周佳伟, 王蕊, 冯敬敬. 2020—2021年中国部分地区猪流行性腹泻病毒S1基因遗传变异分析[J]. 中国畜牧兽医, 2023, 50(6): 2468-2478.
[9]	欧云文, 潘琴, 汪洋, 代军飞, 任绍科, 张洋, 张杰. 猪细小病毒6型ORF2基因的截短表达及多克隆抗体制备[J]. 中国畜牧兽医, 2023, 50(6): 2487-2495.
[10]	潘鹏丞, 雷宗全, 李显, 胡湘云, 秦谦涛, 覃兆鲜, 关志惠, 陈宝剑, 谢炳坤. 陆川猪MYL2基因生物信息学分析、真核表达载体构建及组织表达研究[J]. 中国畜牧兽医, 2023, 50(5): 1796-1806.
[11]	欧阳坤, 魏立民, 孙瑞萍, 解娅立, 齐琪, 李想, 刘圈炜, 晁哲, 张永亮, 陈婷. 姜黄素对海南屯昌猪生长性能、饲料养分表观消化率和血清生化指标的影响[J]. 中国畜牧兽医, 2023, 50(5): 1867-1875.
[12]	张华强, 王品胜, 李晓, 魏攀鹏, 闫灵敏, 蒋兵兵, 王兆贵, 王学方, 曾焕青, 范毅, 高正龙, 王学兵. 发酵杂交构树对湖羊生长性能、肉品质、消化酶活力及肠道菌群结构的影响[J]. 中国畜牧兽医, 2023, 50(5): 1876-1887.
[13]	吴静璇, 孙征, 马雪莲, 张丽阳, 廖秀冬, 罗绪刚, 李素芬, 吕林. 中国畜禽饲料资源中镁含量的调查研究[J]. 中国畜牧兽医, 2023, 50(5): 1888-1898.
[14]	黎明国, 李清春, 陈鑫, 卢世豪, 何凡, 祁梦凡, 张化鹏, 任玉军, 张庆泽, 符彬彬, 徐梦思, 艾子凯, 闫坤, 冯赟, 华再东, 黄涛, 毕延震. TET去甲基化酶对猪卵母细胞发育的影响[J]. 中国畜牧兽医, 2023, 50(5): 1907-1917.
[15]	王泽平, 王飞飞, 张芮铭, 付言峰, 赵为民, 戴超辉, 程金花, 廖超, 李辉. 苏紫猪SLA-1基因多态性及其抗病潜能分析[J]. 中国畜牧兽医, 2023, 50(5): 1918-1927.