骆驼尿液对预防CCl4诱导小鼠肝损伤的保护作用

doi:10.16431/j.cnki.1671-7236.2019.05.008

《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (5): 1316-1322.doi: 10.16431/j.cnki.1671-7236.2019.05.008

骆驼尿液对预防CCl₄诱导小鼠肝损伤的保护作用

浩斯娜¹, 卓娜¹, 海勒¹, 伊丽¹, 明亮¹, 吉日木图^1,2,3

1. 内蒙古农业大学乳品生物技术与工程教育部重点实验室, 呼和浩特 010018;
2. 内蒙古骆驼保护学会, 呼和浩特 010018;
3. 内蒙古骆驼研究院, 巴丹吉林 737300

收稿日期:2018-09-18 出版日期:2019-05-20 发布日期:2019-05-20
通讯作者: 明亮, 吉日木图 E-mail:bmlimau@163.com;yeluotuo1999@vip.163.com
作者简介:浩斯娜(1993-),女,内蒙古鄂尔多斯人,研究生,研究方向:乳制品生物技术与工程,E-mail:hsnimau@163.com
基金资助:
国家自然科学基金项目（31360397）；内蒙古自然科学基金（2018BS03017）；国际科技合作项目（2015DFR30680、ky201401002）

Protective Effect of Camel Urine on Liver Injury Induced by CCl₄ in Mice

HAO Sina¹, ZHUO Na¹, HAI Le¹, YI Li¹, MING Liang¹, JIRIMUTU^1,2,3

1. Key Laboratory of Dairy Biotechnology and Bioengineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Inner Mongolia Camel Conservation Society, Hohhot 010018, China;
3. Inner Mongolia Institute of Camel Research, Badanjiran 737300, China

Received:2018-09-18 Online:2019-05-20 Published:2019-05-20

摘要/Abstract

摘要：

试验旨在研究骆驼尿液对预防四氯化碳（CCl₄）诱导小鼠肝损伤的保护作用。将40只C57BL/6小鼠随机分为正常组，模型组，骆驼尿液低剂量组（170 mg/kg）、中剂量组（340 mg/kg）和高剂量组（500 mg/kg），3组骆驼尿液组灌胃相应剂量的骆驼尿液，正常组和模型组灌胃等量的生理盐水，每日灌胃1次，连续14 d；试验末期，除正常组外（橄榄油），各组小鼠腹腔注射20% CCl₄橄榄油混合溶液，建立小鼠急性肝损伤模型；12 h后摘眼球取血和采集肝脏样本，并记录肝脏重量，计算肝脏指数；测定小鼠血清相关指标，并进行肝脏组织病理切片检查。结果显示，与模型组比较，低、中、高剂量骆驼尿液组小鼠血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）活性及总胆红素（TBIL）、碱性磷酸酶（ALP）、葡萄糖（GLU）含量较模型组均明显降低，白蛋白（ALB）、总蛋白（TP）含量升高，且高剂量组效果较好；而小鼠肝脏病理学切片观察显示，骆驼尿液组均有不同程度的改善，其中高剂量骆驼尿液组小鼠肝脏组织病理学损伤明显减轻，与正常组的肝脏组织形态接近。比较低、中、高3种浓度骆驼尿液处理发现，浓度越高治疗效果越好，说明骆驼尿液治疗效果具有剂量依赖性。综上所述，骆驼尿液对CCl₄诱导小鼠肝损伤具有一定的预防保护作用，且高剂量骆驼尿液组的效果较好。

关键词: 骆驼尿液; 四氯化碳(CCl₄); 肝损伤; 预防作用

Abstract:

In this study,protective effect of camel urine on liver injury induced by CCl₄ in mice was investigated.Forty mice were randomly divided into five groups:Normal group,model group,low-dose camel urine group (170 mg/kg),medium dose camel urine group (340 mg/kg) and high dose camel urine group (500 mg/kg),respectively.The normal group and model group received the same amount of saline infusions once a day,while 3 camel urine groups received different amount of camel urine.All mice were given continuous administration for 14 d and injected with 20% CCl₄ and olive oil solution to establish an acute liver injury model except the control group at the end of the experiment.Mice have sacrificed after 12 h post-CCl₄ injection when blood and livers were collected,and the liver weight and the liver index were recorded and calculated.Then the related serum indicators and the pathological examination of liver tissue were detected.The results showed that compared with the model group,the level of serum ALT,AST,TBIL,ALP,GLU,TP in low,middle,high dose camel urine groups were decreased,while the serum ALB level was increased with high dose group had a better effect.According to the results of the pathological section,the liver histopathological damage of mice in 3 camel urine groups were all alleviate,and that in the high dose camel urine group was very similar to the normal group.In addition,it could be seen from the results that among the three of camel urine groups,the higher concentration had a better therapeutic effect,indicating that the effect of camel urine was dose-dependent.In summary,camel urine had a certain preventive effect on CCl₄-induced liver injury in mice,and the high dose was the better.

Key words: camel urine; carbon tetrachloride (CCl₄); liver injury; prevention

中图分类号:

S852.65

浩斯娜, 卓娜, 海勒, 伊丽, 明亮, 吉日木图. 骆驼尿液对预防CCl₄诱导小鼠肝损伤的保护作用[J]. 《中国畜牧兽医》, 2019, 46(5): 1316-1322.

HAO Sina, ZHUO Na, HAI Le, YI Li, MING Liang, JIRIMUTU. Protective Effect of Camel Urine on Liver Injury Induced by CCl₄ in Mice[J]. , 2019, 46(5): 1316-1322.

参考文献

[1] SALAWA M E,KHONGLI,El-HASSAN A M,et al.Hepatoprotective effect of camel urine against carbon tetrachloride induced hepatotoxicity in rats[J].Journal of Science Technology,2009,10(2):130-137.
[2] ALTHNAIAN T,ALBOKHADAIM I,EL-BAHR S M.Biochemical and histopathological study in rats intoxicated with carbon tetrachloride and treated with camel milk[J].Springerplus,2013,2(1):57.
[3] MOHAMED H E,BEYNEN A C.Vitamin C concentrations in blood plasma,tissues and urine of camels (Camelus dromedarius) in Sudanese herds[J].Journal of Animal Physiology & Animal Nutrition,2002,86(9-10):342-346.
[4] SHABO Y,BARZEL R,MARGOULIS M,et al.Camel milk for food aller-gies in children[J].Israel Medical Association Journal,2005,77:796-259.
[5] 浩斯娜,方舒,卓娜,等.骆驼尿辅助治疗疾病的研究进展[J].中国畜牧兽医,2018,45(11):3279-3285. HAO S N,FANG S,ZHUO N,et al.Research progress on camel urine adjuvant treatment of diseases[J].China Animal Husbandry & Veterinary Medicine,2018,45(11):3279-3285.(in Chinese)
[6] AHMADA,ALABBASI F A,SADATH S.Ameliorative effect of camel's milk and nigella sativa oil against thioacetamide-induced hepatorenal damage in rats[J].Pharmacognosy Magazine,2018,14(53):27-35.
[7] 马齐襄.实时检测药物肝损伤过程中氧化应激反应的可视化小鼠模型的建立[D].北京:北京中医药大学,2018. MA Q X.Visualization of oxidative stress-induced hepatotoxicity by Real-time imaging[D].Beijing:Beijing University of Chinese Medicine,2018.(in Chinese)
[8] 宁康健,吕锦芳,姜锦鹏,等.黄芩苷对小鼠四氯化碳肝损伤保护作用的研究[J].中国中医药科技,2011,18(2):120-122. NING K J,LYU J F,JIANG J P,et al.Study on protective effect of baicalin on mice' hepatic injury caused by CCl₄[J].Chinese Journal of Traditional Medical Science and Technology,2011,18(2):120-122.(in Chinese)
[9] 张玲莉,彭燕.急性肝损伤模型建立与血清生化指标相关性研究[J].中国药师,2015,18(9):1460-1463. ZHANG L L,PENG Y.Establishment of acute hepatic injury model in mice and correlation analysis of serum biochemical parameters[J].Chinese Pharmacist,2015,18(9):1460-1463.(in Chinese)
[10] ALTAA S,KÄZÄ L G,KÄZÄ L M,et al.Protective effect of DiyarbakÄr watermelon juice on carbon tetrachloride-induced toxicity in rats[J].Food & Chemical Toxicology,2011,49(9):2433-2438.
[11] ZHAN W,LIAO X,TIAN T,et al.Study on the effects of blueberry treatment on histone acetylation modification of CCl₄-induced liver disease in rats[J].Genetics & Molecular Research,2017,16(1):16.
[12] 陈伟.木蹄复方水提物对四氯化碳肝损伤的保护作用及其机制[D].上海:第二军医大学,2013. CHEN W.The protective effect and its mechanism of aqueous extract from Fomes fomentarius compound on carbon tetrachloride induced liver injury[D].Shanghai:Second Military Medical University,2013.(in Chinese)
[13] 谢志芳.锌指蛋白Zbtb20肝细胞特异性基因敲除小鼠对CCl₄肝损伤的敏感性及其机理研究[D].上海:第二军医大学,2010. XIE Z F.Sensitivity of Zbtb20 hepatocyte specific gene knockout mice to CCl₄ liver injury and its mechanism[D].Shanghai:Second Military Medical University,2010.(in Chinese)
[14] 徐红丽,王静,韩丽华,等.α、β、γ、δ-生育酚对小鼠急性四氯化碳肝损伤保护作用的研究[J].营养学报,2017,39(3):270-274. XU H L,WANG J,HAN L H,et al.Protection of α,β,γ and δ-tocopherols against acute liver injury induced by carbon tetrachloride in mice[J].Acta Nutrimenta Sinica,2017,39(3):270-274.(in Chinese)
[15] 于佳民,谢全喜,张建梅,等.中草药复合微生态制剂对急性肝脏损伤蛋鸡肝脏保护作用的研究[J].中国畜牧兽医,2016,43(7):1904-1909. YU J M,XIE Q X,ZHANG J M,et al.Study on liver-protective effect of chinese herbal compound probiotics on acute liver injury layers[J].China Animal Husbandry & Veterinary Medicine,2016,43(7):1904-1909.(in Chinese)
[16] AL-YOUSEF N,GAAFAR A,Al-OTAIBI B,et al.Camel urine components display anti-cancer properties in vitro[J].Journal of Ethnopharmacology,2012,143(3):819-825.
[17] MOSTAFA M,DWEDAR R.Antimicrobial activity of camel's urine and its effect on multidrug resistant clinical bacterial and fungal isolates[J].British Journal of Pharmaceutical Research,2016,13(4):2231-2919.
[18] AL-SHAMI S A,EL-HABASHI N,MANDOUR M A,et al.The potential effect of camel milk and urine against CCl₄-induced lesions in male rats[J].Journal of Animal & Veterinary Advances,2014,13(20):1160-1166.
[19] AL-FARTOSI K.Role of camel's urine in growth rate of Chroococcus sp.[J].European Journal of Pharmaceutical & Medical Research,2016,3(4):9-12.
[20] ROMLI F,ABU N,KHORSHID F A,et al.Thegrowth inhibitory potential and antimetastatic effect of camel urine on breast cancer cells in vitro and in vivo[J].Integrative Cancer Therapies,2017,16(4):540-555.

骆驼尿液对预防CCl₄诱导小鼠肝损伤的保护作用

Protective Effect of Camel Urine on Liver Injury Induced by CCl₄ in Mice

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 13

编辑推荐

Metrics

本文评价

[1]	沈幸玲, 丁康宁, 王幽丛, 张易安, 刘逸雷, 刘涵笑, 唐陆平, 何永明. 火炭母缓解鼠伤寒沙门菌致小鼠肝损伤的机制研究[J]. 中国畜牧兽医, 2022, 49(7): 2768-2777.
[2]	安志霞, 潘阳阳, 范碧玥, 马小燕, 叶得河, 张治杰, 邱山桐, 姚亚乐, 王萌. 基于网络药理学研究连翘叶提取物通过AMPK抗恩诺沙星诱导肝损伤的作用[J]. 中国畜牧兽医, 2022, 49(6): 2347-2361.
[3]	赵伟捷, 孙耀贵, 孙盼盼, 孙娜, 杨惠珍, 李宏全. 黑种草子提取物对小鼠酒精性肝损伤的修复作用及机制研究[J]. 中国畜牧兽医, 2022, 49(12): 4928-4935.
[4]	蒙锦燕, 徐百昌, 司红彬. 葛根素对对乙酰氨基酚诱导的急性肝损伤的保护作用研究[J]. 中国畜牧兽医, 2022, 49(11): 4457-4465.
[5]	赵晨栋, 王萌, 张昊, 代国年, 安志霞, 沈雅丽, 王桂荣. 连翘提取物对对乙酰氨基酚诱导小鼠肝损伤的保护作用[J]. 中国畜牧兽医, 2021, 48(10): 3845-3854.
[6]	杨浩, 金三俊, 庞倩, 冯兴军. 白藜芦醇对畜禽常见肝损伤保护机制及应用的研究进展[J]. 中国畜牧兽医, 2020, 47(11): 3528-3535.
[7]	浩斯娜, 方舒, 卓娜, 刘丽君, 李彤, 王瑞雪, 明亮, 吉日木图. 骆驼尿辅助治疗疾病的研究进展[J]. 《中国畜牧兽医》, 2018, 45(11): 3279-3285.
[8]	艾力·艾尔肯, 库尔班江·麦麦提敏, 蒋志惠, 张小莺, 米克热木·沙衣布扎提. 刺糖对对乙酰氨基酚诱导的肝损伤的保护作用[J]. 《中国畜牧兽医》, 2017, 44(7): 2185-2190.
[9]	叶伟庆, 杜炳旺, 李东华, 李乃宾. 高温应激致麒麟鸡(卷羽鸡)肝损伤试验动物模型的建立[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(3): 741-744.
[10]	刘红微;赵丽芹;张田田;沈明浩;李沐灵. 土庄绣线菊水浸液对小鼠急性酒精肝损伤保护作用的研究[J]. , 2012, 39(4): 166-168.
[11]	张世栋;王东升;李世宏;李宏胜;荔霞;李锦宇;陈炅然;龚成珍;严作廷. 芩连液与白虎汤对脂多糖致家兔肝损伤的疗效比较[J]. , 2011, 38(7): 183-186.
[12]	赵良中;时文艳;鞠晓红;李妍. 玉竹提取物A对小鼠免疫性肝损伤治疗作用的研究[J]. , 2010, 37(3): 160-163.
[13]	马惠茹;赵玉娥;郝向阳;赵智香;程建国. 沙棘护肝作用的研究[J]. , 2009, 36(8): 202-203.