2013~2017年江西省猪圆环病毒2型分子流行病学调查

doi:10.16431/j.cnki.1671-7236.2019.04.022

摘要/Abstract

摘要：

为掌握江西地区猪圆环病毒2型（PCV2）的分子流行病学及其遗传变异情况，本研究运用实验室已建立的PCR方法对2013~2017年采集自江西省南昌市、宜春市、新余市、赣州市、吉安市、九江市、上饶市、抚州市、景德镇市、鹰潭市等10个地区的1 082份疑似PCV2感染猪病料组织进行病原学检测，并通过PCR扩增、克隆和基因测序对PCV2的全基因组序列进行分析。研究表明，1 082份病料中有610份为PCV2阳性，总阳性率为56.4%，其中2013~2017年阳性率分别为52.7%、48.8%、58.5%、71.0%和67.4%。共获得89株PCV2全基因组序列，其中有83株为PCV2b基因型，其中53株归类于PCV2b-1C基因亚群，30株归为PCV2b-1A/1B基因亚群；6株为PCV2a型。测序毒株与参考毒株ORF2基因核苷酸、氨基酸序列同源性分别为88.9%~100.0%和86.0%~100.0%；氨基酸序列分析表明，Cap蛋白存在20个氨基酸突变位点。本研究表明，江西地区猪群中PCV2的主导基因型为PCV2b，其中又以PCV2b-1C基因亚群占据主导地位，同时也存在少量PCV2a基因型。

关键词: 江西省; 猪圆环病毒2型(PCV2); 全基因组; 分子流行病学调查

Abstract:

To investigate the molecular epidemiology and genetic diversity of porcine circovirus type 2 (PCV2) in Jiangxi province,1 082 tissue samples were collected during 2013 to 2017 from suspected pigs in 10 regions (including Nanchang,Yichun,Xinyu,Ganjiang,Ji'an,Jiujiang,Shangrao,Fuzhou,Jingdezhen and Yingtan cities) of Jiangxi province were detected for PCV2.And PCV2 isolates complete genome sequences were amplified,cloned and sequenced.The results showed that 610 of 1 082 samples were detected to be positive of PCV2 and the total positive rate was 56.4%.The positive rates from 2013 to 2017 were 52.7%,48.8%,58.5%,71.0% and 67.4%,respectively.The 83 of 89 PCV2 isolates belonged to PCV2b,and 53 and 30 of 83 isolates were grouped into PCV2b-1C and PCV2b-1A/1B,respectively,and 6 isolates belonged to PCV2a.The homology of nucleotides and the deduced amino acid sequence of ORF2 between sequenced strains and reference strains were 88.9% to 100.0% and 86.0% to 100.0%,respectively.Analysis of Cap acid amino sequences showed that there were 20 amino acid mutation sites.This research indicated that the dominant genotype of PCV2 in pigs in Jiangxi was PCV2b,and PCV2b-1C was a dominant subgenotype.At the same time,a small amount of PCV2a subgenotype also existed.

Key words: Jiangxi province; porcine circovirus type 2 (PCV2); complete genome; molecular epidemiological investigation

中图分类号:

S852.65

蒋新华, 周荷田, 罗锋, 杨丹凤, 张志庆, 陈松昌, 邓舜洲. 2013~2017年江西省猪圆环病毒2型分子流行病学调查[J]. 《中国畜牧兽医》, 2019, 46(4): 1143-1150.

JIANG Xinhua, ZHOU Hetian, LUO Feng, YANG Danfeng, ZHANG Zhiqing, CHEN Songchang, DENG Shunzhou. Molecular Epidemiological Investigate of Porcine Circovirus Type 2 in Jiangxi Province During 2013 to 2017[J]. , 2019, 46(4): 1143-1150.

参考文献

[1] DELWART E,LI L.Rapidly expanding genetic diversity and host range of the Circoviridae viral family and other recoding small circular ssDNA genomes[J].Virus Research,2012,164(1-2):114-121.
[2] CHAE C.An emerging porcine circovirus type 2b mutant (mPCV2b) originally known as PCV2d[J].Veterinary Journal,2015,203(1):6-9.
[3] NOVOSEL D,TUBOLY T,CSAGOLA A,et al.Origin of porcine circovirus type 2(PCV2) from swine affected by PCV2-associated diseases in Croatia[J].Veterinary Record,2014,174(17):431.
[4] KOINIG H C,TALKER S C,STADLER M,et al.PCV2 vaccination induces IFN-γ/TNF-α co-producing T cells with a potential role in protection[J].Veterinary Research,2015,46:20-24.
[5] 邓静,赵春容,曹三杰,等.2010-2013年四川地区猪圆环病毒2型的分子流行病学调查[J].中国兽医学报,2014,34(12):1868-1876. DENG J,ZHAO C R,CAO S J,et al.Molecular epidemiological investigate of porcine circovirus type 2 between 2010 and 2013 in some regions of Sichuan[J].Chinese Journal of Veterinary Science,2014,34(12):1868-1876.(in Chinese)
[6] TISCHER I,BODE L,PETERS D,et al.Distribution of antibodies to porcine circovirus in swine populations of different breeding farms[J].Archives of Virology,1995,140(4):737-743.
[7] FRANZO G,CORTEY M,SEGALES J,et al.Phylodynamic analysis of porcine circovirus type 2:Methodological approach and datasets[J].Data in Brief,2016,8:549-552.
[8] OLVERA A,CORTEY M,SEGALES J.Molecular evolution of porcine circovirus type 2 genomes:Phylogeny and clonality[J].Virology,2007,357(2):175-185.
[9] 李玲,李国新,周艳君,等.2008-2011年中国部分地区猪圆环病毒2型的分子流行病学调查[J].中国动物传染病学报,2012,20(2):1-10. LI L,LI G X,ZHOU Y J,et al.Molecular epidemiological investigation into porcine circovirus type 2 between 2008 and 2011 in some regions of China[J].Chinese Journal of Animal Infectious Diseases,2012,20(2):1-10.(in Chinese)
[10] AN D J,ROH I S,SONG D S,et al.Phylogenetic characterization of porcine circovirus type 2 in PMWS and PDNS Korean pigs between 1999 and 2006[J].Veterinary Research,2007,129(2):115-122.
[11] FRANZO G,CORTEY M,OLVERA A,et al.Revisiting the taxonomical classification of porcine circovirus type 2(PCV2):Still a real challenge[J].Virology Journal,2015,12:131.
[12] 吴瑷,章红兵,王鑫炎,等.2007~2012年浙江省及周边地区猪圆环病毒2型(PCV2)分子流行病学调查[J].农业生物技术学报,2013,21(4):456-463. WU Y,ZHANG H B,WANG X Y,et al.Molecular epidemiological investigation of porcine circovirus type 2(PCV2) in Zhejiang province and surrounding areas[J].Journal of Agricultural Biotechnology,2013,21(4):456-463.(in Chinese)
[13] HARMON K M,GAUGER P C,ZHANG J,et al.Whole-genome sequences of novel porcine circovirus type 2 viruses detected in swine from Mexico and the United States[J].Genome Announcement,2015,3(6):1315.
[14] GILLESPIE J,OPRIESSNIG T,MENG X J,et al.Porcine circovirus type 2 and porcine circovirus-associated disease[J].Journal of Veterinary Internal Medicine,2009,23(6):1151-1163.
[15] 廖启顺,李志祥,丁联成,等.2009-2010年部分猪场主要传染病流行病学调查[J].中国畜牧兽医,2012,39(2):194-199. LIAO Q S,LI Z X,DING L C,et al.Epidemiological research of major infectious disease from partial pig farm from 2009 to 2010[J].China Animal Husbandry & Veterinary Medicine,2012,39(2):194-199.(in Chinese)
[16] 邵萌,吕亚辉,杜谦,等.2010-2012年陕西地区猪圆环病毒2型分子流行病学调查[J].西北农林科技大学学报(自然科学版),2013,41(10):19-26. SHAO M,LYU Y H,DU Q,et al.Molecular epidemiological investigation on porcine circovirus type 2 in Shaanxi province during 2010-2012[J].Journal of Northwest A & F University (Natural Science Edition),2013,41(10):19-26.(in Chinese)
[17] SEGALES J,OLVERA A,GRAU-ROMA L,et al.PCV-2 genotype definition and nomenclature[J].Veterinary Record,2008,162(26):867-868.
[18] 王利丽,郑丽,路超,等.猪圆环病毒2型天津及周边地区分离株的遗传演化分析[J].中国畜牧兽医,2018,45(5):1331-1340. WANG L L,ZHENG L,LU C,et al.Phylogenetic analysis of porcine circovirus type 2 isolate from Tianjin and its surrounding areas[J].China Animal Husbandry & Veterinary Medicine,2018,45(5):1331-1340.(in Chinese)
[19] 戴爱玲,刘建奎,李鸿文,等.闽西地区猪圆环病毒2型血清学调查及遗传变异分析[J].中国兽医学报,2016,36(6):896-901. DAI A L,LIU J K,LI H W,et al.Serological investigation and genetic diversity of porcine circovirus type 2 infection in swine farms of Western Fujian pro-vince[J].Chinese Journal of Veterinary Science,2016,36(6):896-901.(in Chinese)
[20] 时庆贺,石昂,李新果,等.河南省2016-2017年致病性猪圆环病毒遗传演化分析[J].中国兽医学报,2018,38(5):857-862. SHI Q H,SHI A,LI X G,et al.Phylogenetic analysis of prevailing pathogenic porcine circovirus in Henan province during 2016-2017[J].Chinese Journal of Veterinary Science,2018,38(5):857-862.(in Chinese)
[21] 徐朋丽,张宇,韩昊莹,等.猪圆环病毒2型河南株全基因组的克隆与遗传进化分析[J].中国预防兽医学报,2016,38(12):989-992. XU P L,ZHANG Y,HAN H Y,et al.Cloning and phylogenetic evolution analysis of the complete genomes of porcine circovirus type 2 from Henan province[J].Chinese Journal of Preventive Veterinary Medicine,2016,38(12):989-992.(in Chinese)
[22] KWEON C H,NGUYEN L T,YOO M S,et al.Differential recognition of the ORF2 region in a complete genome sequence of porcine circovirus type 2(PCV2) isolated from boarbone marrow in Korea[J].Gene,2015,569(2):308-312.
[23] 赵军,周桐枫,刘鹏娟,等.四川地区腹泻仔猪群猪圆环病毒Ⅱ型分子流行病学调查[J].中国预防兽医学报,2016,38(7):546-549. ZHAO J,ZHOU T F,LIU P J,et al.Molecular epidemiology of porcine circovirus typeⅡ in diarrhea piglets in Sichuan region[J].Chinese Journal of Preventive Veterinary Medicine,2016,38(7):546-549.(in Chinese)