瘤胃保护性5-羟基色氨酸对绵羊肠道内容物褪黑素含量及菌群结构的影响

doi:10.16431/j.cnki.1671-7236.2019.03.007

摘要/Abstract

摘要：

本试验旨在研究不同水平瘤胃保护性5-羟基色氨酸对绵羊胃肠道内容物中褪黑素含量及细菌多样性的影响，探究通过5-羟基色氨酸调节绵羊胃肠道细菌多样性的可能性。试验选取3岁、平均体重为（47.79±3.70） kg的健康哈萨克母羊15只，按体重相近原则随机分为3组，每组5只，分别为对照组和Ⅰ、Ⅱ组，每天每只羊的粉状精料饲喂量为1.2%（以体重计），玉米青贮为1.8 kg，混合干草自由采食，在此基础上，Ⅰ、Ⅱ组试验羊分别饲喂111、222 mg/（kg·BW）瘤胃保护性5-羟色氨酸，进行25 d的饲养试验。试验结束当天晨饲后6 h后立即宰杀全部绵羊，取其空肠、回肠、结肠、盲肠内容物测定褪黑素含量及菌群结构。结果表明，Ⅰ组结肠内容物，Ⅱ空肠、结肠内容物中褪黑素含量均极显著高于对照组（P<0.01），Ⅰ、Ⅱ组回肠、盲肠内容物中褪黑素含量均低于对照组，但差异不显著（P>0.05）。Ⅰ、Ⅱ组空肠和盲肠内容物细菌菌群ACE、Chaol指数均低于对照组（P>0.05）。3组盲肠、结肠内容物中细菌多样性在门、属水平上均无显著差异（P>0.05）。而与对照组相比，Ⅰ组空肠内容物细菌中厚壁菌门相对丰度显著增加（P<0.05），无壁菌门相对丰度显著降低（P<0.05）；在属水平上，Ⅱ组空肠内容物中Aeriscardovia属相对丰度显著增加（P<0.05）。在回肠内容物中，Ⅰ、Ⅱ组疣微菌属Ruminococcaceae-NK4A214相对丰度显著高于对照组（P<0.05），而梭状芽胞杆菌属、Romboutsia的相对丰度均显著低于对照组（P<0.05）。综上所述，瘤胃保护性5-羟基色氨酸使成年绵羊结肠内容物中褪黑素含量增加，但对肠道菌群多样性无显著影响。

关键词: 绵羊; 胃肠道; 内容物; 瘤胃保护性5-羟基色氨酸; 褪黑素; 细菌多样性

Abstract:

This study was aimed to investigate the effects of rumen protected 5-hydroxytryptophan (RPT-5-HTP) supplementation on melatonin content and microbial communities in gastrointestinal tract digesta of sheep,and explored the possibility of regulating sheep gastrointestinal tract microbial communities by RPT-5-HTP.Fifteen Kazak sheep which were 3 year old,average body weight (47.79±3.70) kg were chosen and divided into 3 groups:Control group,groups Ⅰ and Ⅱ.All sheep were fed with 12 g/(kg·BW) powder concentrate,1.8 kg corn silage every day,and had free continuous access to mixed hay.Additionally,each sheep in groups Ⅰ and Ⅱ was fed with RPT-5-HTP 111,222 mg/(kg·BW),respectively.The supplementary feeding experiment lasted for 25 d.At the end of the experiment,all sheep were slaughtered after the morning feeding (6 h),and the contents of jejunal,ileal,colonic and cecal digesta were taken to determine melatonin content and microflora structure.The results showed that the melatonin concentration in colonic digesta content of groups Ⅰ and jejunal and colonic digesta of groups Ⅱ were extremely significantly higher than that in control group (P<0.01),and that in ileal and cecal digesta of groups Ⅰ,Ⅱ were lower than control group,but there was no significant differences among three groups (P>0.05).The ACE and Chaol indexes of bacterial flora in the jejunal,cecal digesta of groups Ⅰ,Ⅱ were lower than those in control group (P>0.05).Compared with the control group,the bacterial diversity in cecal and colonic digesta were not significantly different at the levels of phylum and genus (P>0.05).The relative abundance of Firmicutes in the jejunal digesta was significantly increased in group Ⅰ (P<0.05),and the relative abundance of Tenericutes in group Ⅰ was significantly lower than that in control group (P<0.05).At the genus level,the relative abundance of Aeriscardovia in the jejunal digesta of group Ⅱ was increased (P<0.05).The relative abundance of Ruminococcaceae-NK4A214 in ileal digesta of groups Ⅰ,Ⅱ were significantly increased (P<0.05),while relative abundance of Clostridium-sensu-stricto and Romboutsia were significantly decreased (P<0.05).In conlusion,the RPT-5-HTP could increase melatonin concentration in the colonic digesta,but had no significant effect on the bacterial diversity of the gastrointestinal tract.

Key words: sheep; gastrointestinal tract; digesta; RPT-5-HTP; melatonin; bacterial diversity

中图分类号:

S823

赵芳, 王根, 赵国栋, 高超, 李晓斌, 马晨, 杨开伦. 瘤胃保护性5-羟基色氨酸对绵羊肠道内容物褪黑素含量及菌群结构的影响[J]. 《中国畜牧兽医》, 2019, 46(3): 690-702.

ZHAO Fang, WANG Gen, ZHAO Guodong, GAO Chao, LI Xiaobin, MA Chen, YANG Kailun. Effect of Supplemented with Rumen-protected 5-hydroxytryptophan on Melatonin Content and Microbial Communities in Intestinal Tract Digesta of Sheep[J]. , 2019, 46(3): 690-702.

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