转录组测序技术在猪繁殖与呼吸综合征病毒研究中的应用

doi:10.16431/j.cnki.1671-7236.2019.01.005

摘要/Abstract

摘要：

猪繁殖与呼吸综合征病毒（porcine reproductive and respiratory syndrome virus，PRRSV）能导致母猪繁殖障碍及仔猪呼吸困难等症状，严重威胁全球养猪业的健康发展。目前，PRRSV的致病机制及其与宿主的互作机理并不完全清楚。近年来，随着高通量测序技术的快速发展，转录组测序技术已被广泛应用于PRRSV的研究中，为深入解析PRRSV的致病机理及其与宿主的互作机制提供了一种全新的研究工具。作者从感染应答、蛋白功能、毒株、疫苗、抗病品种5个方面对近年来转录组测序技术在PRRSV研究中的应用进行了综述分析，对其筛选的差异表达基因（DEGs）和通路进行总结，以期为未来PRRSV转录组学研究提供参考。

关键词: 猪繁殖与呼吸综合征病毒(PRRSV); 转录组测序; 差异表达基因; 通路

Abstract:

Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of reproductive failure in sows and respiratory disorders in piglets.The spread and outbreak of PRRSV has led to a serious threat to global pig industry.Currently,the pathogenic mechanism and interaction with the hosts of PRRSV remains unclear.With the rapid development of high throughput sequencing,the transcriptome sequencing has been widely applied in the research of PRRSV.It offers a new way for the explanation of the pathogenic mechanism and interaction with the hosts of PRRSV.This article reviews the application of transcriptome sequencing technology in the response,the function of virus proteins,the strains,the vaccine and the resistance breeding of PRRSV research,it summarizes the differentially expressed genes (DEGs) and pathways enriched in the transcriptome sequencing of PRRSV infection,and may provide a reference for PRRSV research in the future.

Key words: porcine reproductive and respiratory syndrome virus (PRRSV); transcriptome sequencing technology; differentially expressed genes; pathways

中图分类号:

S852.4

梁婉, 周丹娜, 彭忠, 杨克礼, 段正赢, 郭锐, 刘威, 吴斌, 陈文杰, 孟丽, 田永祥. 转录组测序技术在猪繁殖与呼吸综合征病毒研究中的应用[J]. 《中国畜牧兽医》, 2019, 46(1): 37-44.

LIANG Wan, ZHOU Danna, PENG Zhong, YANG Keli, DUAN Zhengying, GUO Rui, LIU Wei, WU Bin, CHEN Wenjie, MENG Li, TIAN Yongxiang. Application of Transcriptome Sequencing Technology in Porcine Reproductive and Respiratory Syndrome Virus Research[J]. , 2019, 46(1): 37-44.

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