绵羊附睾褪黑素受体1的表达与定位

doi:10.16431/j.cnki.1671-7236.2018.09.026

摘要/Abstract

摘要：

为研究褪黑素受体1（MT1）在不同年龄绵羊附睾中的表达模式，选用幼龄绵羊（2~3月龄）、青年绵羊（6~8月龄）和成年绵羊（2~3岁）的附睾，采用实时荧光定量PCR和免疫组化技术检测不同年龄绵羊附睾各部位MT1基因mRNA的表达量和MT1的分布情况。结果表明：幼龄绵羊附睾尾MT1基因的转录水平极显著高于附睾头和附睾体（P<0.01）；青年绵羊附睾体和附睾尾MT1基因的转录水平显著高于附睾头（P<0.05）；成年绵羊附睾尾MT1基因的转录水平显著高于附睾头和附睾体（P<0.05），附睾各部位MT1基因的转录水平随年龄增长呈明显降低趋势；定位结果显示，MT1在各年龄组绵羊附睾的各个部位均有分布，且主要分布在附睾上皮细胞中。综合上述结果，不同年龄绵羊附睾的不同部位均有MT1表达和分布，并随年龄增长各部位的表达量降低，相同年龄绵羊附睾尾MT1的表达水平较高。

关键词: 绵羊; 附睾; 褪黑素受体1(MT1); 基因表达; 蛋白定位

Abstract:

In order to study the expression pattern of melatonin receptor 1 (MT1) in epididymis of sheep at different age,epididymis were collected from lamb (2 to 3 months old),young ram (6 to 8 months old) and adult sheep (2 to 3 years old),and the expression of MT1 mRNA and the distribution of MT1 in different parts of the epididymis were detected by the Real-time quantitative PCR and immunohistochemistry.The results showed that the MT1 transcription level in the lambs cauda was extremely significantly higher than that in the caput and corpus (P<0.01).The MT1 transcription level in corpus and cauda of young ram were significantly higher than that in the caput (P<0.05).The transcription level of MT1 in adult ram cauda was significantly higher than that in the caput and corpus (P<0.05).The transcription level of MT1 in epididymal decreased with age.The immunohistochemistry results showed that MT1 could be detected in all parts of sheep epididymis and mainly distributed in the epididymal epithelial cells.It was concluded that MT1 were expressed and distributed in different parts of the epididymis of sheep at different age,and the MT1 expression in epididymis decreased with age.The MT1 expression level was higher in cauda than other parts of epididymis for sheep at the same age.

Key words: sheep; epididymis; melatonin receptor 1(MT1); gene expression; protein localization

中图分类号:

S814.8

宋慧子, 边小娜, 栗瑞兰, 刘春洁, 张家新. 绵羊附睾褪黑素受体1的表达与定位[J]. 《中国畜牧兽医》, 2018, 45(9): 2544-2549.

SONG Huizi, BIAN Xiaona, LI Ruilan, LIU Chunjie, ZHANG Jiaxin. Expression and Localization of Melatonin Receptor 1 in Sheep Epididymis[J]. , 2018, 45(9): 2544-2549.

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