伏牛白山羊TACR1基因扩增及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2018.09.008

摘要/Abstract

摘要：

本研究旨在克隆伏牛白山羊TACR1基因，并对其结构和编码蛋白进行生物信息学分析，为进一步研究TACR1基因的功能及其在生殖生理和免疫调节中的作用奠定基础。根据GenBank中已发表的山羊TACR1基因（登录号：NC_030818.1）序列设计引物，对伏牛白山羊TACR1基因进行分段PCR扩增并测序，拼接后得到包含TACR1基因完整编码区的序列片段。结果表明，伏牛白山羊TACR1基因开放阅读框（ORF）全长852 bp，共编码283个氨基酸，碱基组成为：A（23.47%）、T（25.49%）、C（27.76%）和G（23.27%）。与绵羊相比，伏牛白山羊TACR1基因编码区发生了2个碱基突变，但没有引起氨基酸的改变。5'端非编码序列长为860 bp，发生了9个碱基突变；3'端非编码序列长为271 bp，发生了2个碱基突变。同源性分析结果显示，伏牛白山羊与卡普拉山羊、藏羚羊、绵羊、瘤牛、白尾鹿、白鳍豚、人、野猪、金钱豹、土拨鼠、野驴TACR1基因同源性分别为99.9%、99.3%、99.2%、97.5%、96.7%、92.9%、89.7%、88.6%、86.3%、86.2%和85.0%。SignalP 4.1在线软件预测结果显示，TACR1 N末端存在信号肽且可能在21位氨基酸处存在裂解位点；蛋白质结构预测发现伏牛白山羊TACR1基因编码蛋白没有跨膜结构域。

关键词: 伏牛白山羊; TACR1基因; 生物信息学分析

Abstract:

The purpose of this study was to clone the TACR1 gene of Funiu White goat and analyze its structure and encoded protein by bioinformatics methods to lay a foundation for further study on the function of TACR1 gene and its role in reproductive physiology and immune regulation.5 pairs of primers were designed based on the goat TACR1 gene sequence in GenBank (accession No:NC_030818.1).The Funiu White goat TACR1 gene was amplified by PCR amplification and sequenced,and the complete coding region (CDS) of TACR1 gene was obtained after splicing the fragments.The results showed that the open reading frame(CRF) of TACR1 gene in Funiu White goat was 852 bp in length and encoded a total of 283 amino acids.The content of base composition was A (23.47%),T (25.49%),C (27.76%) and G (23.27%).Compared with sheep TACR1 gene, the TACR1 gene CDS region of the Funiu White goat had a two-base mutation which did not cause amino acid changes.The non-coding region at the 5'and 3' ends was 860 and 271 bp in length, and had 9 and 2 base mutations,respectively.The results of homology analysis showed that the identity of TACR1 gene between Funiu White goat and Capra hircus,Pantholops hodgsonii,Ovis aries,Bos indicus,Odocoileus virginianus,Lipotes vexillifer,Homo sapiens,Sus scrofa,Panthera pardus,Cavia porcellus,Equus asinus were 99.9%,99.3%,99.2%,97.5%,96.7%,92.9%,89.7%,88.6%, 86.3%,86.2% and 85.0%,respectively.There was a signal peptide at the N-terminus of TACR1 and there might be a cleavage site at 21th amino acid predicted by SignalP4.1 online software.Through the prediction of protein structure,it was found that the protein encoded by TACR1 gene of the FuNiu White goat had no transmembrane domain.

Key words: Funiu White goat; TACR1 gene; bioinformatics analysis

中图分类号:

Q785

施会彬, 王玉琴, 田志龙, 张小辉, 王建平, 杨芳, 李元晓. 伏牛白山羊TACR1基因扩增及生物信息学分析[J]. 《中国畜牧兽医》, 2018, 45(9): 2409-2416.

SHI Huibin, WANG Yuqin, TIAN Zhilong, ZAHNG Xiaohui, WANG Jianping, YANG Fang, LI Yuanxiao. Amplification and Bioinformatics Analysis of TACR1 Gene in Funiu White Goat[J]. , 2018, 45(9): 2409-2416.

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