《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (8): 2197-2203.doi: 10.16431/j.cnki.1671-7236.2018.08.019

• 动物营养与饲料科学 • 上一篇    下一篇

复合植物精油对脂多糖诱导的断奶仔猪免疫应激的影响

徐海旺1, 余魁1, 吴梦郡1, 刘文凯1, 李鹏1, 易丹1,2, 王蕾1,2, 侯永清1,2   

  1. 1. 武汉轻工大学动物营养与饲料科学湖北省重点实验室, 武汉 430023;
    2. 武汉轻工大学动物营养与饲料安全湖北省协同创新中心, 武汉 430023
  • 收稿日期:2017-11-26 出版日期:2018-08-20 发布日期:2018-08-15
  • 通讯作者: 王蕾 E-mail:wagnlei_wh@aliyun.com
  • 作者简介:徐海旺(1991-),男,安徽安庆人,硕士生,研究方向:营养生化与代谢调控,E-mail:1059989886@qq.com
  • 基金资助:

    武汉市科技计划项目(2016020101010080);国家重点研发计划专项(2016YFD0501210);湖北省技术创新专项(重大项目)(2016ABA121)

The Effect of Compound Plant Essential Oil on LPS-induced Immune-stressed Weaned Pigs

XU Haiwang1, YU Kui1, WU Mengjun1, LIU Wenkai1, LI Peng1, YI Dan1,2, WANG Lei1,2, HOU Yongqing1,2   

  1. 1. Hubei key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China;
    2. Hubei Collaborative Innovation Center of Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, China
  • Received:2017-11-26 Online:2018-08-20 Published:2018-08-15

摘要:

试验旨在研究复合植物精油(OCT)对脂多糖(LPS)诱导的断奶仔猪的免疫应激的影响。试验选取28日龄左右的杜×长×大三元杂交仔猪,随机分成3个处理组:对照组(基础日粮,注射生理盐水),LPS组(基础日粮,注射LPS),OCT组(基础日粮+50 mg/kg OCT,注射LPS)。每组8个重复,每个重复1头猪。于试验第21天注射LPS(100 μg/kg体重)或等体积的生理盐水,注射3 h后前腔静脉采血进行白细胞分类计数;6 h后屠宰,取胸腺、脾脏,-80℃保存,用于测量胸腺、脾脏免疫相关基因白介素-4(IL-4)、白介素-6(IL-6)、白介素-10(IL-10)、肿瘤坏死因子(TNF-α)、核因子(NF-κB)以及转录相关基因转录激活因子3(STAT3)的基因相对表达量。结果表明:①与对照组相比,LPS刺激降低了仔猪血液中白细胞、中性粒细胞、淋巴细胞、单核细胞及嗜酸性粒细胞数量(P<0.05);与LPS组相比,日粮中添加OCT提高了白细胞的数量(P<0.05);②与对照组相比,LPS刺激上调了脾脏、胸腺IL-6、STAT3 mRNA水平(P<0.05),下调了脾脏IL-4、TNF-α及胸腺IL-4、TNF-α、NF-κB mRNA水平(P<0.05);与LPS组相比,添加OCT下调了脾脏、胸腺IL-6、STAT3 mRNA水平(P<0.05);上调了胸腺IL-4、IL-10、TNF-α、NF-κB的mRNA水平(P<0.05)。综合上述结果,LPS诱导发生了仔猪免疫应激,OCT在一定程度具有缓解作用。

关键词: 复合植物精油(OCT); 断奶仔猪; 脂多糖(LPS); 免疫应激

Abstract:

The purpose of this research was to study the effect of OCT on the immune stress of weaned piglets induced by LPS.A total of 24 weaned piglets (Duroc×Large white×Landrace) were randomly assigned to three treatments at 28 days:Control group (based diet,injection of saline),LPS group (basic diet,injection of LPS) and OCT group (basal diet+50 mg/kg OCT,injection of LPS).Each treatment included 8 piglets that were subdivided equally to 8 pens,each pen had 1 piglets.On 21th day of the experiment,piglets in the LPS and OCT groups were injected intraperitoneally with LPS (100 μg/(kg·BW)),whereas piglets in the control group were injected intraperitoneally with the same volume of physiological saline.Blood samples were obtained 3 h after injection for analysis of white blood cell differential counts.6 h after LPS challenged,the pigs were slaughtered and thymus and spleen were taken for measuring the related genes IL-4,IL-6,IL-10,TNF-α,NF-κB,and STAT3 mRNA.The result showed that:①Compared with control group,LPS stimulation could lead to a significant reduction in the number of the eosinophils,neutrophils,lymphocytes,monocytes,white blood cells in the blood (P<0.05).Compared with LPS group,adding OCT could significantly increase blood levels of white blood cells (P<0.05).②Compared with control group,after injection of LPS,mRNA levels of IL-6 and STAT3 genes significantly increased in spleen and thymus (P<0.05);mRNA levels of IL-4,TNF-α in spleen and IL-4,TNF-α,NF-κB genes mRNA levels in thymus had been significantly reducted (P<0.05).Compared with LPS group,adding OCT could significantly reduce IL-6 and STAT3 mRNA levels in both spleen and thymus (P<0.05),and significantly increase IL-4,IL-10,TNF-α and NF-κB genes mRNA levels in thymus (P<0.05).In conclusion,LPS could induce immune stress in weaned pigs,whereas OCT could improve its immunity and alleviate inflammation to some extent.

Key words: compound plant of essential oil (OCT); piglets; lipopolysaccharide(LPS); immune stressed

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