一株新疆野鸟源新城疫病毒的分离鉴定、F基因克隆及遗传进化分析

doi:10.16431/j.cnki.1671-7236.2017.09.030

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (9): 2755-2760.doi: 10.16431/j.cnki.1671-7236.2017.09.030

一株新疆野鸟源新城疫病毒的分离鉴定、F基因克隆及遗传进化分析

张慧敏¹, 木哈买提江·铁格斯¹, 史军², 吴桂灵¹, 古丽扎提·阿地理², 加尔肯¹, 苏战强¹, 冉多良¹, 沙依兰·卡依扎², 兰玲³, 姚刚¹, 刘建华¹

1. 新疆农业大学动物医学学院, 家畜传染病实验室, 乌鲁木齐 830052;
2. 新疆维吾尔自治区林业厅野生动植物保护管理处, 乌鲁木齐 830000;
3. 新疆畜牧科学院兽医研究所, 乌鲁木齐 830011;
4. 新疆维吾尔自治区动物卫生监督所, 乌鲁木齐 830063

收稿日期:2017-02-15 出版日期:2017-09-20 发布日期:2017-09-22
通讯作者: 姚刚, 刘建华 E-mail:yaogang516@163.com;liujianhua627@126.com
作者简介:张慧敏(1992-),女,新疆昌吉人,硕士,研究方向:分子病毒学,E-mail:474572439@qq.com;木哈买提江·铁格斯(1988-),男,新疆阿勒泰人,硕士,研究方向:分子病毒学,E-mail:1078741254@qq.com
基金资助:
2015年新疆农业大学研究生科研创新项目（XJAUGRI2015016）；新疆农业大学2017年校级大学生创新项目

Isolation and Identification, F Gene Cloning and Evolutionary Analysis of Newcastle Disease Virus Isolated from Wild Birds in Xinjiang

ZHANG Hui-min¹, Muhamaitijiang·T¹, SHI Jun², WU Gui-ling¹, Gulizhati·Adili², JIA Er-ken¹, SU Zhan-qiang¹, RAN Duo-liang¹, Shayilan·Kayizha², LAN Ling³, YAO Gang¹, LIU Jian-hua¹

1. Laboratory of Animal Infectious Disease, College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China;
2. The Management Office of the Wildlife Protection of Forestry Bureau of Xinjiang Uygur Automous Region, Urumqi 830000, China;
3. Institute of Veterinary Medicine, Xinjiang Academy of Animal Science, Urumqi 830011, China;
4. Animal Health Supervision Institute of Xinjiang, Urumqi 830063, China

Received:2017-02-15 Online:2017-09-20 Published:2017-09-22

摘要/Abstract

摘要：

为了解新疆野鸟新城疫病毒（Newcastle disease virus，NDV）感染情况，分析其分子特征和基因变异特点，防止新城疫的暴发和蔓延，本试验用9日龄SPF鸡胚进行病毒的分离传代，HA、HI试验和RT-PCR方法对位于"东非-西亚迁徙线"福海县的野鸟进行了NDV检测，分离到1株野鸟源NDV，并命名为NDV/Pintail/CH（XJ）/01/2016。结果表明，该NDV分离株F基因ORF长1 662 bp，编码553个氨基酸，F基因碱性裂解位点序列为¹¹²G-R-Q-G-R-L¹¹⁷，符合弱毒株特征；遗传进化分析显示，NDV/Pintail/CH（XJ）/01/2016与乌克兰鸽源分离株Doneck/3/968、比利时鸭源分离株Simeonovgrad核苷酸同源性均为99.7%，属于NDV ClassⅡ基因Ⅱ型。而上述3株病毒均与疫苗毒La Sota具有较高同源性（99.5%~99.8%）。本研究结果为家禽NDV外流进入自然环境提供了论据。

关键词: 野鸟; 新城疫病毒(NDV); F基因; 遗传进化分析

Abstract:

In order to understand inflection of Newcastle disease virus (NDV) of Xinjiang wild birds, through analysis of molecular characteristics and genetic variation, to prevent the outbreak and spread of Newcastle disease, isolation of the virus was performed in 9 days old specific pathogen free (SPF) chicken embryos, detected the wild birds in Fuhai county which was located in the migration line of East Africa-West Asia by HA, HI test and RT-PCR method. And then 1 strain of NDV from wild birds was isolated, named as NDV/Pintail/CH(XJ)/01/2016. As the result, ORF of F gene from the NDV isolate was 1 662 bp, encoding 553 amino acids. The motif of the cleavage site was ¹¹²G-R-Q-G-R-L¹¹⁷, which was consistent with the characteristics of avirulent NDV strains. The phylogenetic analysis showed that NDV/Pintail/CH(XJ)/01/2016 nucleotide sequence homologies of F gene between reference strain Doneck/3/968 and mule Simeonovgrad strain homology were high and reached 99.7%, it belonged to genotype Ⅱ of Class Ⅱ NDV. All of the three viruses had high homology to the vaccine strain La Sota,which were 99.5% to 99.8%. According to this research conclusion we provided evidence that poultry NDV outflowed into the natural environment.

Key words: wild birds; Newcastle disease virus (NDV); F gene; phylogenetic analysis

中图分类号:

S852.65⁺7

张慧敏, 木哈买提江·铁格斯, 史军, 吴桂灵, 古丽扎提·阿地理, 加尔肯, 苏战强, 冉多良, 沙依兰·卡依扎, 兰玲, 姚刚, 刘建华. 一株新疆野鸟源新城疫病毒的分离鉴定、F基因克隆及遗传进化分析[J]. 《中国畜牧兽医》, 2017, 44(9): 2755-2760.

ZHANG Hui-min, Muhamaitijiang·T, SHI Jun, WU Gui-ling, Gulizhati·Adili, JIA Er-ken, SU Zhan-qiang, RAN Duo-liang, Shayilan·Kayizha, LAN Ling, YAO Gang, LIU Jian-hua. Isolation and Identification, F Gene Cloning and Evolutionary Analysis of Newcastle Disease Virus Isolated from Wild Birds in Xinjiang[J]. , 2017, 44(9): 2755-2760.

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一株新疆野鸟源新城疫病毒的分离鉴定、F基因克隆及遗传进化分析

Isolation and Identification, F Gene Cloning and Evolutionary Analysis of Newcastle Disease Virus Isolated from Wild Birds in Xinjiang

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