《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (4): 1182-1188.doi: 10.16431/j.cnki.1671-7236.2017.04.034

• 预防兽医 • 上一篇    下一篇

内蒙古中东部地区不同来源大肠杆菌耐药性调查

李海华1, 郭蔚冰1, 杨海明1, 翁伟平1, 闫强2, 张远达1, 陈志强1   

  1. 1. 内蒙古华天制药有限公司, 赤峰 024070;
    2. 乌兰察布市动物疫病预防控制中心, 乌兰察布 012000
  • 收稿日期:2016-08-08 出版日期:2017-04-20 发布日期:2017-05-03
  • 通讯作者: 陈志强 E-mail:Zhq_chen@163.com
  • 作者简介:李海华(1987-),女,内蒙古赤峰人,硕士,研究方向:大肠杆菌耐药性,E-mail:nmglihaihua@163.com
  • 基金资助:

    2013年国家中小企业创新基金(13C26211500785)

Investigation on Antibiotics Resistance of Escherichia coli Isolated fromDifferent Sources in the Middle East Region of Inner Mongolia

LI Hai-hua1, GUO Wei-bing1, YANG Hai-ming1, WENG Wei-ping1, YAN Qiang2, ZHANG Yuan-da1, CHEN Zhi-qiang1   

  1. 1. Inner Mongolia Hua Tian Pharmaceutical Co., Ltd., Chifeng 024000, China;
    2. Animal Disease Prevention and Control Center of Wulanchabu City, Wulanchabu 012000, China
  • Received:2016-08-08 Online:2017-04-20 Published:2017-05-03

摘要:

本研究旨在调查内蒙古赤峰、通辽地区不同来源的大肠杆菌对抗生素类药物的耐药情况。采用K-B法分析来源于鹅、牛和猪等患病动物分离出的46株大肠杆菌对13种常用的抗生素类药物的耐药性;鹅源和猪源大肠杆菌对盐酸多西环素的耐药率最高,分别达95.2%和93.3%;牛源大肠杆菌对盐酸沙拉沙星、恩诺沙星和盐酸多西环素的耐药率最高,达80.0%。多重耐药性结果表明,患病动物的大肠杆菌以6耐(23.9%)为主;牛源大肠杆菌则以7耐(40%)最多。总体耐药谱型以恩诺沙星/氟苯尼考/盐酸多西环素/阿莫西林/硫酸新霉素为主(24.4%)。不同来源的大肠杆菌对抗生素类药物均产生了严重的耐药性,因此不但要求养殖人员学会合理使用抗生素类药物,同时研究开发逆转耐药性的新药物或对抗生素类药物有增敏效用的新药物十分必要。

关键词: 抗菌药; 大肠杆菌; 耐药性; K-B法

Abstract:

The study was aimed to research the antibiotics resistance of Escherichia coli (E.coli) isolated from different sources of Chifeng and Tongliao areas in Inner Mongolia.Using K-B method, we analysed the resistance situation of 46 strains E.coli isolated from goose, bovine and porcine to thirteen kinds of antibiotic drugs. The resistance rates of E.coli isolated from goose and porcine had the highest resistance to doxycycline hydrochloride drug, which were 95.2% and 93.3%,respectively. The resistant rate of E.coli isolated from bovine had the highest resistance to sarafloxacinhydrochloride,enrofloxacin and hydrochloric acid doxycycline, which was 80.0%. Multi-resistance results showed that the prevalence of E.coli in diseased animals with 6 resistance mainly(23.9%);Bovine source with 7 resistance (40%). The resistance type in general was enrofloxacin/florfenicol/doxycycline/amoxicillin/neomycin sulfate. E.coli from different sources of diseased animals to antibiotics had a serious resistance. So,it is not only necessary for farmers to learn to use antibiotics rationally, but also to develop new drugs that are resistant to drug resistance or new drug allergic to antibiotics.

Key words: antibacterial drug; Escherichia coli; resistance; K-B method

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