《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (3): 825-831.doi: 10.16431/j.cnki.1671-7236.2017.03.029

• 遗传繁育 • 上一篇    下一篇

基因多态性与超声波活体测定相结合对肉牛主要肉质性状的研究

李娜1,2, 李红波1, 周振勇1, 张金山1, 闫向民1, 杜玮1, 袁理星1, 张杨1   

  1. 1. 新疆畜牧科学院, 乌鲁木齐 830000;
    2. 甘肃农业大学, 兰州 730070
  • 收稿日期:2016-08-11 出版日期:2017-03-20 发布日期:2017-03-21
  • 通讯作者: 张杨 E-mail:zhyang1962@126.com
  • 作者简介:李娜(1985-),女,新疆乌鲁木齐人,博士生,助理研究员,研究方向:肉牛遗传育种,Tel:0991-3075095;E-mail:550806052@qq.com
  • 基金资助:

    现代农业产业技术体系建设专项资金(nycytx-38);自治区科技重大专项课题"新疆肉牛新品种(系)培育与杂交改良技术研究示范"(201230116-10)

Study on Genetic Polymorphism and Ultrasound Measurements for Major Meat Quality Traits in Beef Cattle

LI Na1,2, LI Hong-bo1, ZHOU Zhen-yong1, ZHANG Jin-shan1, YAN Xiang-min1, DU Wei1, YUAN Li-xing1, ZHANG Yang1   

  1. 1. Xinjiang Academy of Animal Sciences, Urumqi 830000, China;
    2. Gansu Agricultural University, Lanzhou 730070, China
  • Received:2016-08-11 Online:2017-03-20 Published:2017-03-21

摘要:

本试验采用PCR-SSCP与超声波活体测定相结合的方法,对Leptin基因外显子Ⅱ E2JW的SNP位点与宰前/宰后新疆褐牛背膘厚度、眼肌面积、脂肪含量、眼肌高度的部分肉质性状进行关联性分析。结果表明,外显子Ⅱ E2JW位点的PIC处于中度多态(0.25 < PIC < 0.5),E2JW多态位点存在3个基因型:AA、AB和BB,其中AB为优势基因型,B为优势等位基因。相关性结果表明,除预测值中AB、AA基因型个体的肌肉脂肪含量与实测值的分析结果不一致外,其他均表现出新疆褐牛Leptin基因外显子Ⅱ E2JW多态性与预测肉质性状和实测肉质性状关系的一致性。因此,Leptin基因外显子Ⅱ E2JW是影响新疆褐牛肉质性状的候选基因位点,基因多态性结合超声波活体测定技术不仅可以快速、准确地测量部分肉质性状,而且可作为分子标记辅助选择的一种手段。

关键词: 新疆褐牛; Leptin基因; 外显子Ⅱ E2JW位点; 遗传多态性; 超声波; 肉质性状

Abstract:

In this study,we used PCR-SCCP connect with ultrasonic vivo assay techniques, analysis of correlation between Leptin gene exon Ⅱ E2JW polymorphism and part meat quality traits of backfat thickness, eye muscle area, fat content, plot eye height in Xinjiang Brown cattle at ante-mortem, post-mortem. The results showed that Leptin gene exon Ⅱ E2JW site PIC was moderate polymorphisms (0.25 < PIC < 0.5), there were three genotypes of AA, AB and BB, AB genotype were the predominant genotype, B was the predominant allele. In addition, the forecast value of AB and AA gennotypes individual muscle fat content were inconsistent with actual value.Leptin gene exon Ⅱ E2JW was identified the theroy of a candidate gene loci of beef quality traits in Xinjiang Brown cattle.Genetic polymorphism and ultrasound measurements could not only fastly and accurately measure portion meat traits, but also as a means of marker-assisted selection.

Key words: Xinjiang Brown cattle; Leptin gene; exon Ⅱ E2JW site; genetic polymorphism; ultrasonic; meat quality traits

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