新疆奎屯地区不同团场牛源大肠杆菌耐药情况比较

doi:10.16431/j.cnki.1671-7236.2016.08.030

摘要/Abstract

摘要：

为了解新疆奎屯地区不同团场牛源大肠杆菌对临床常用抗菌药物的耐药情况，比较不同养殖场之间牛源大肠杆菌的耐药差异，分别从新疆奎屯地区位于123团、127团和128团的牛场，用肛拭子在直肠采集粪样各30份，并分离大肠杆菌。采用微量肉汤稀释法，对分离出的大肠杆菌进行10种抗菌药物最小抑菌浓度的测定，通过卡方检验对不同养殖场牛源大肠杆菌耐药差异性进行比较分析。结果显示，128团对阿莫西林/克拉维酸（33.3%）和安普霉素（23.3%）耐药率相对较高；127团对氨苄西林（20.0%）、阿莫西林/克拉维酸（16.7%）和氟苯尼考（16.7%）耐药率相对较高；123团场对氨苄西林（13.3%）和安普霉素（13.3%）耐药率相对较高。卡方检验结果显示，127团对氟苯尼考和恩诺沙星的耐药率显著高于123团和128团（P<0.05）；128团对阿莫西林/克拉维酸和安普霉素的耐药率分别显著高于123团和127团（P<0.05）。不同团场多重耐药均以1～2耐为主。结果表明，新疆奎屯地区不同团场牛源大肠杆菌对多数被检药物的耐药率不高，仅对常用药物如阿莫西林/克拉维酸和安普霉素等具有较高耐药率，需在临床治疗细菌疾病时更换或避免使用此类抗菌药物。此外，各养殖场对被检抗菌药物的耐药情况不同，说明养殖场的用药极大程度影响细菌的耐药性。

关键词: 大肠杆菌; 抗菌药物; 微量肉汤稀释法; 耐药率

Abstract:

In order to understand the resistance of E.coli to clinical antimicrobial drugs from different farms in Kuitun area of Xinjiang,the bovine fecal samples were collected using the anal swab and E.coli was isolated from 123 (30),127 (30) and 128 (30) farms,respectively.The minimum inhibitory concentrations of E coli isolated from fecal samples to 10 antimicrobial drugs were determined by agar dilution method and the difference of resistance rate of E.coli from different farms was compared by chi square test.The results showed that the resistance rates of 128 farm to amoxicillin/clavulanic acid (33.3%) and apramycin (23.3%) were relatively high;The resistance rates of 127 farm to ampicillin (20.0%),amoxicillin/clavulanic acid (16.7%) and florfenicol (16.7%) were relatively high;The resistance rates of 123 farm to ampicillin (13.3%) and the apramycin (13.3%) were relatively high.The chi square test results showed that resistance rates of E.coli to florfenicol and enrofloxacin from 127 farm were significantly higher than that from 123 and 128 farms (P<0.05);The resistance rates of E.coli to amoxicillin/clavulanic acid and apramycin from 128 farm were significantly higher than that from 123 and 127 farms (P<0.05),respectively.The most of the antibiotics resistance pattern of E.coli from different farms was 1 to 2 resistant.The results showed that the drug resistance rates of bovine E.coli in Kuitun was not high,while the resistance rates to commonly used drugs such as amoxicillin/clavulanic acid and apramycin were high.It suggested that this kinds of antimicrobial drugs should be replaced or avoid using to treat bacterial diseases.In addition,the antimicrobial resistance between farms was different,indicating the farms medicine would impact the drug resistance in bacteria.

Key words: E.coli; antimicrobial drugs; broth dilution method; resistance rate

中图分类号:

S852.61

轩慧勇, 高超, 林亚军, 夏绪进, 夏利宁, 王营彬, 马炀. 新疆奎屯地区不同团场牛源大肠杆菌耐药情况比较[J]. 《中国畜牧兽医》, 2016, 43(8): 2150-2155.

XUAN Hui-yong, GAO Chao, LIN Ya-jun, XIA Xu-jin, XIA Li-ning, WANG Ying-bin, MA Yang. Comparison of Drug Resistance of Escherichia coli from Different Cattle Farms in Kuitun Area of Xinjiang[J]. , 2016, 43(8): 2150-2155.

参考文献

[1] 陆承平.兽医微生物学[M].第4版.北京:中国农业出版社,2011.
[2] Aarestrup F M,Oliver D C,Burch D G.Antimicrobial resistance in swine production[J].Animal Health Research Reviews,2008,9(2):135-148.
[3] 植婵萍,郭小婷,姚旭,等.有机农场鸡、猪源大肠杆菌耐药性研究[J].中国畜牧兽医,2015,42(12):3351-3357.
[4] Clinical and Laboratory Standards Institute.Performance Standards for Antimicrobial Susceptibility Testing;Twentieth Informational Supplement[S].CLSI documents M100-S22,2012.
[5] 郭庆勇,南海辰,夏利宁,等.新疆伊犁某牛场不同生长期牛粪源大肠杆菌耐药性调查[J].中国农学通报,2015,31(2):27-30.
[6] 底丽娜.新疆不同动物源大肠杆菌耐药性调查及其相关耐药基因检测[D].石河子:新疆农业大学,2014.
[7] 梁宏儒.黑龙江部分奶牛场大肠杆菌耐药性和耐药基因检测分析[D].黑龙江:黑龙江八一农垦大学,2013.
[8] 余婷,谢敏,冀智佩,等.宁夏地区牛源大肠埃希菌ESBLs检测及耐药性分析[J].动物医学进展,2013,34(3):25-28.
[9] 李路,刘继超,姜铁民,等.北京地区奶牛养殖场中大肠杆菌的分离鉴定及耐药性分析[J].中国乳品工业,2014,12:7-9.
[10] Cummings K J,Aprea V A,Altier C.Antimicrobial resistance trends among Escherichia coli isolates obtained from dairy cattle in the Northeastern United States,2004-2011[J].Foodborne Pathogens and Disease,2014,11(1):61-67.
[11] Barlow R,McMillan K,Duffy L,et al.Prevalence and antimicrobial resistance of Salmonella and Escherichia coli from Australian cattle populations at slaughter[J].Jouranl of Food Protection,2015,78(5):912-920.
[12] 夏利宁,向发,郭庆勇,等.新疆不同地区牛源大肠杆菌耐药性分析[J].中国畜牧兽医,2014,41(2):203-207.
[13] 姚琼芬,陈孝杰,田伟,等.猪源和牛源大肠杆菌耐药性比较试验[J].中国兽医杂志,2011,47(8):32-34.
[14] 郑龙龙,刘萍丹,赵德育,等.云南省奶牛乳房炎致病菌分离鉴定及耐药性分析[J].黑龙江畜牧兽医,2015,16:86-89.
[15] Ogunleye A O,Okunlade A O,Jeminlehin F O,et al.Antibiotic resistance in Escherichia coli isolated from healthy cattle at a major cattle market in Ibadan,Oyo state,Southwestern,Nigeria[J].African Journal of Microbiology Research,2013,7(33):4572-4575.
[16] 赖婧,刘洋,汪宇,等.800株不同动物源大肠杆菌的耐药性监测[J].中国兽医杂志,2011,4:12-14.
[17] 许丹丹,许追,齐向涛,等.石河子地区隐性乳房炎奶牛源大肠杆菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2015,42(3):701-707.
[18] 夏绪进,程伟华,夏利宁,等.同一养殖模式下不同地区猪源大肠杆菌耐药性比较[J].中国畜牧兽医,2015,42(8):2189-2193.
[19] 南海辰,夏利宁,刘英玉,等.新疆塔城某规模化养殖场大肠埃希菌耐药性调查[J].动物医学进展,2014,35(9):118-123.
[20] 武瑞兵,高玉敏,王鹏翔,等.牛肉源大肠杆菌的耐药性检测及相关耐药基因分布[J].中国畜牧兽医,2015,42(2):452-458.
[21] Mainali C,McFall M,King R,et al.Evaluation of antimicrobial resistance profiles of Escherichia coli isolates of broiler chichens at slaughter in Alberta Canada[J].Jouranl of Food Protection,2013,76(12):2045-2051.