›› 2015, Vol. 42 ›› Issue (12): 3274-3281.doi: 10.16431/j.cnki.1671-7236.2015.12.024

• 遗传繁育 • 上一篇    下一篇

新疆褐牛近交群体的鉴定研究

马力鹏1, 黄涛1, 卢国昌2, 李秀英2, 卢振宇2, 翟腾蛟1, 程悦聪1, 冯书堂3   

  1. 1. 石河子大学动物科技学院, 石河子 832000;
    2. 塔城恰夏褐牛养殖场, 塔城 834700;
    3. 中国农业科学院北京畜牧兽医研究所, 北京 100193
  • 修回日期:2015-10-20 出版日期:2015-12-20 发布日期:2015-12-30
  • 通讯作者: 黄涛, 冯书堂 E-mail:taohuang100@sina.com;fst508@sina.com
  • 作者简介:马力鹏(1989-),男,甘肃武威人,硕士,研究方向:动物遗传育种与繁殖,E-mail:1355474932@qq.com
  • 基金资助:
    国家自然科学基金(31460586);教育部留学归国人员科研启动基金((2013)1092)

Study on Identification of Xinjiang Brown Cattle Inbred Group

MA Li-peng1, HUANG Tao1, LU Guo-chang2, LI Xiu-ying2, LU Zhen-yu2, ZHAI Teng-jiao1, CHENG Yue-cong1, FENG Shu-tang3   

  1. 1. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China;
    2. Brown Cattle Farms of Tacheng Qiaxia, Tacheng 834700, China;
    3. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Revised:2015-10-20 Online:2015-12-20 Published:2015-12-30

摘要: 试验旨在检测新疆褐牛近交群体的遗传相似性和群体的纯合度。利用13对微卫星引物对由2头新疆褐牛经40年完全封闭繁育出的55头新疆褐牛近交群体和25头对照组新疆褐牛的遗传结构特征进行了比对分析,以期开展对近交群体的鉴定研究。结果发现,新疆褐牛近交群体的纯合度为0.9064,多态信息含量为0.0873,可知该群体具有低多态性;对照组新疆褐牛的纯合度为0.4647,多态信息含量为0.4882。将该近交群体按毛色分为4个亚群:A、B、C和D,各亚群的遗传纯合度分别为0.9029、0.9632、0.9208和0.9551。结果表明受检测的新疆褐牛近交群体有较高的纯合度和很低的杂合性,已成为遗传纯度很高的近交群体。该封闭的近交新疆褐牛群体是中国牛品种中难得、宝贵的遗传资源。

关键词: 新疆褐牛; 近交群体; 遗传相似性; 纯合度

Abstract: This study was aimed to detect the genetic similarity and groups homozygosity of Xinjiang Brown cattle inbred group.Use 13 pairs of microsatellite primers to structure of genetics in the comparative analysis by the two Xinjiang Brown cattle over 40 years completely closed breeding out 55 Xinjiang Brown cattles group and 25 Xinjiang Brown cattles in control group,in order to carry out the identification of inbred groups.For Xinjiang Brown inbred group,the homozygosity was 0.9064,the polymorphism information content was 0.0873,it was found that the groups had a low polymorphism;The homozygosity was 0.4647,and polymorphic information content was 0.4882 in Xinjiang Brown cow of control group.According to the coat color of the inbreeding groups was divided into four subgroups A,B,C and D.The genetic purity of each subgroup were 0.9029,0.9632,0.9208 and 0.9551.The results showed that the detection of Xinjiang Brown cattle inbred group had higher homozygosity and lower heterozygosity,had became a high genetic purity of inbreeding group.The closure of Xinjiang Brown cattle inbred group was rare breeds of cattle,and was valuable genetic resources.

Key words: Xinjiang Brown cattle; inbred groups; genetic similarity; homozygosity

中图分类号: